| Definition | Methanosarcina mazei Go1 chromosome, complete genome. |
|---|---|
| Accession | NC_003901 |
| Length | 4,096,345 |
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The map label for this gene is topA [H]
Identifier: 21226285
GI number: 21226285
Start: 254024
End: 256522
Strand: Direct
Name: topA [H]
Synonym: MM_0183
Alternate gene names: 21226285
Gene position: 254024-256522 (Clockwise)
Preceding gene: 21226283
Following gene: 21226287
Centisome position: 6.2
GC content: 47.98
Gene sequence:
>2499_bases ATGACAGTTGTCGCATTTGCAGAAAAAAACAAAGCAGCAGCCCAGATCGCCAGCATCCTTGGCGAAGGGGAAGTGGATAA AATATCAGTGGAAGGGCTGCCTGCCTACGAGTTTAAATGGAAAGGAGAAGAGTGGCTTGTGATGGGGCTTTCCGGCCACA TCATGAACTACGACTTTCCAGAAAAATATAACAAATGGAGTGAAGTTGACCCCGGGACACTGCTTGGAGTTGATCCGGAA AAATTTGTGACCAGGAAGGAGTATGAAGCTGCAATAAAAAACCTGGCAAAAAGGGCAGACAAAATTATCCTTGCATGCGA CTATGACAGGGAAGGGGAAAATATAGGGTTTGAAGCAAAAACCCTTGCCGAAGAGGTCACTGATGTCCCTGTTGCAAGGG CACGTTTTTCAGCCCTTTCCCCAAAAGAAGTAAGGAAAGCCTTTGAAAACCCTGTAGATCCTGACCACAACCTTGCTATG GCTGCCGAAGCCAGGCAGATTCTTGACCTGAAGATGGGGGCTGCATTTACACGTTTTGTTACGCTCTCGGTCAGGGAAAA AGCAAGGACAAAAGACGTCCTTTCAATAGGGCCCTGCCAGACTCCTACCTGCGGGTTTGTTTACGAAAGGGAAAAGGCTA TCCGGGCTTTTGAGGCAAAGGACTTCTGGAAGATTACTGCGGTTTTTGCTGCAAAAGGAGGGGAATTCGAAGGCACCCAC AGGGCAGGAAACATCCATGACAAAGAAAAGGCAGCTGAAATCTTCAAGAGGCTGAAAGGGGCAAAAGAAGGACTCGTTGT AAAGAAGACCGTAAAAGAAGCAAAAACCAGCCCTCCAAATCCACTTAACACCACAGAGTTCCTGAAGCGGGCTTCAAAGT TCCTGGGGATCAGCCCCGAAGTTGCACTCGAAACCGCAGAACAGCTCTACCTTGCAGGGTTTACGAGCTACCCGAGGACA GAGACCAATAAATATGCAGATGACTTTGACTTCAAGTCCCTGGTCTTTGATTTCGCAAGGCAGAAAGAGTATAAGCCATT TGCAGAATCAATCCTCTCTGCCCCGATAGTTCCAAAGAATGGAGAAAAAGACGCACATGACCACCCACCTATCCACCCGA TCAGGGCGGCTGCAAGGGGAGAGGTCAGTTCTGCAGTAAATATACCGCAGGCTGCTGAAGTTTATGACCTTATTGCAAGG CATTTCCTGGCAAACCTCATGCCTGCAGCAGTTTTCGAAAAAACCCACCTCCACCTGCAGGTTAAGGAAGAGCCTTTCGA CTCCTCGGGGACCGTGCTTAAAGAACCGGGATGGCTTGAGGTTTATCCGTTTGAAAATAAAAAAGATAAGCTTCTCCCGT TTGTAGAGGAAAAAGAGAGAGTAGACGTAAAAAAGCTGAGCAATACAAAATCCAAGACCAGCCCTCCGAAAAAACTGACA GAAGCCGAACTCCTGACCCTTATGGACAGGCACGGGATAGGGACAAAAGCAACAGCCCCTACACATATAGAAACAAACAA AAAGAGGGGGTATTTCGAGAATAAAGGAAAGACAATTTCAATCTTAGATACAGGTTTTACCCTTATGGAAGGGCTTTCCC TGTCCGTCCCTATCCTTGTAAAACCTGATATCCGCTCAAAGATAGAAGCCCTTATCCAGGAAGTTGAAGACGGGAAAAAA GAGTTCGAAGCGGCTCTTGCCGATGGTACGGTCCTGATTAAAGAGATGTATGCCCAGCTTGAAGCAAACAAAAAAGAGCT TACCTCAAACATTGCAGGCACAATAAAGGATGAAGCCGCCCTCGAAGACAAGAAAAATTACATCGGGACATGTAAGGCTT GCGGGCACGTTCTCAGAATCATACAAACGGATTCCGGGCGTTTTGTGGGCTGCACAGGCTATCCTGACTGCAGAAACTCC TTCTCTCTACCCAAGACCGGAGCCCTTACCGTGCTCAGGAGCAAGGAATGTAAAAAAGAAGGGGCAGCCGTCCTGAAGGT GGGAAACAAATACAACTGGGCTGTTGGTATTGGACCCTGCTTTAAATGCGAGTTTGAAAAGGAGTGCTATCCCCCTGAAA CCGTGGGACCCTGCCCTGAATGTGACGGAAGCATGTTCCTGATATCTTTCAAAGACAACCGCTTCCTGGGCTGTACAAAG CGTTGTGGATATACCCATTCCGTTCCTAAAACCGGAAAACTGACCCTCCTCGACAAAACCTGTGAGACCTGCGGCTGGAA GCTGTTCAGGCTGAAAGAAGAGGTAGAAGGAAACGAAAGCCTGGAAGAAGATATCTGCATAAACCGGCGCTGCACTGAAG GAATAAAGTACTGGAAAAAAGTGGGACCCAGAAGTGGAGCAAGAGCACAGGGCAAGATTTCTGCAGACTCTGGAGCAGAG CTCGGGGCGAGAAGTACGGCAAGAAATAAGGAAAGGACGACGGCTCGCAGAGTTTCAAAAACTGTATCAGTGGCAGACAG CAGGAAAGACCGGAAGTGA
Upstream 100 bases:
>100_bases GAACATTTTTACGCACAAAATTCATTAAAATTTTAGAATTGTTCCGTTACTTTTCCTTACGAAATTCCATATCACATTAA CAGAGGTTAAGGTACTTTAT
Downstream 100 bases:
>100_bases AGGATTTTTAAGAGGGCCGAAAAATGAAGTTATAGAGTTATAATATCTCTACCGTGCAACCTTACTGGCTATACAGAGCC GTTCCTGTACGCTCGACGAA
Product: DNA topoisomerase III
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 832; Mature: 831
Protein sequence:
>832_residues MTVVAFAEKNKAAAQIASILGEGEVDKISVEGLPAYEFKWKGEEWLVMGLSGHIMNYDFPEKYNKWSEVDPGTLLGVDPE KFVTRKEYEAAIKNLAKRADKIILACDYDREGENIGFEAKTLAEEVTDVPVARARFSALSPKEVRKAFENPVDPDHNLAM AAEARQILDLKMGAAFTRFVTLSVREKARTKDVLSIGPCQTPTCGFVYEREKAIRAFEAKDFWKITAVFAAKGGEFEGTH RAGNIHDKEKAAEIFKRLKGAKEGLVVKKTVKEAKTSPPNPLNTTEFLKRASKFLGISPEVALETAEQLYLAGFTSYPRT ETNKYADDFDFKSLVFDFARQKEYKPFAESILSAPIVPKNGEKDAHDHPPIHPIRAAARGEVSSAVNIPQAAEVYDLIAR HFLANLMPAAVFEKTHLHLQVKEEPFDSSGTVLKEPGWLEVYPFENKKDKLLPFVEEKERVDVKKLSNTKSKTSPPKKLT EAELLTLMDRHGIGTKATAPTHIETNKKRGYFENKGKTISILDTGFTLMEGLSLSVPILVKPDIRSKIEALIQEVEDGKK EFEAALADGTVLIKEMYAQLEANKKELTSNIAGTIKDEAALEDKKNYIGTCKACGHVLRIIQTDSGRFVGCTGYPDCRNS FSLPKTGALTVLRSKECKKEGAAVLKVGNKYNWAVGIGPCFKCEFEKECYPPETVGPCPECDGSMFLISFKDNRFLGCTK RCGYTHSVPKTGKLTLLDKTCETCGWKLFRLKEEVEGNESLEEDICINRRCTEGIKYWKKVGPRSGARAQGKISADSGAE LGARSTARNKERTTARRVSKTVSVADSRKDRK
Sequences:
>Translated_832_residues MTVVAFAEKNKAAAQIASILGEGEVDKISVEGLPAYEFKWKGEEWLVMGLSGHIMNYDFPEKYNKWSEVDPGTLLGVDPE KFVTRKEYEAAIKNLAKRADKIILACDYDREGENIGFEAKTLAEEVTDVPVARARFSALSPKEVRKAFENPVDPDHNLAM AAEARQILDLKMGAAFTRFVTLSVREKARTKDVLSIGPCQTPTCGFVYEREKAIRAFEAKDFWKITAVFAAKGGEFEGTH RAGNIHDKEKAAEIFKRLKGAKEGLVVKKTVKEAKTSPPNPLNTTEFLKRASKFLGISPEVALETAEQLYLAGFTSYPRT ETNKYADDFDFKSLVFDFARQKEYKPFAESILSAPIVPKNGEKDAHDHPPIHPIRAAARGEVSSAVNIPQAAEVYDLIAR HFLANLMPAAVFEKTHLHLQVKEEPFDSSGTVLKEPGWLEVYPFENKKDKLLPFVEEKERVDVKKLSNTKSKTSPPKKLT EAELLTLMDRHGIGTKATAPTHIETNKKRGYFENKGKTISILDTGFTLMEGLSLSVPILVKPDIRSKIEALIQEVEDGKK EFEAALADGTVLIKEMYAQLEANKKELTSNIAGTIKDEAALEDKKNYIGTCKACGHVLRIIQTDSGRFVGCTGYPDCRNS FSLPKTGALTVLRSKECKKEGAAVLKVGNKYNWAVGIGPCFKCEFEKECYPPETVGPCPECDGSMFLISFKDNRFLGCTK RCGYTHSVPKTGKLTLLDKTCETCGWKLFRLKEEVEGNESLEEDICINRRCTEGIKYWKKVGPRSGARAQGKISADSGAE LGARSTARNKERTTARRVSKTVSVADSRKDRK >Mature_831_residues TVVAFAEKNKAAAQIASILGEGEVDKISVEGLPAYEFKWKGEEWLVMGLSGHIMNYDFPEKYNKWSEVDPGTLLGVDPEK FVTRKEYEAAIKNLAKRADKIILACDYDREGENIGFEAKTLAEEVTDVPVARARFSALSPKEVRKAFENPVDPDHNLAMA AEARQILDLKMGAAFTRFVTLSVREKARTKDVLSIGPCQTPTCGFVYEREKAIRAFEAKDFWKITAVFAAKGGEFEGTHR AGNIHDKEKAAEIFKRLKGAKEGLVVKKTVKEAKTSPPNPLNTTEFLKRASKFLGISPEVALETAEQLYLAGFTSYPRTE TNKYADDFDFKSLVFDFARQKEYKPFAESILSAPIVPKNGEKDAHDHPPIHPIRAAARGEVSSAVNIPQAAEVYDLIARH FLANLMPAAVFEKTHLHLQVKEEPFDSSGTVLKEPGWLEVYPFENKKDKLLPFVEEKERVDVKKLSNTKSKTSPPKKLTE AELLTLMDRHGIGTKATAPTHIETNKKRGYFENKGKTISILDTGFTLMEGLSLSVPILVKPDIRSKIEALIQEVEDGKKE FEAALADGTVLIKEMYAQLEANKKELTSNIAGTIKDEAALEDKKNYIGTCKACGHVLRIIQTDSGRFVGCTGYPDCRNSF SLPKTGALTVLRSKECKKEGAAVLKVGNKYNWAVGIGPCFKCEFEKECYPPETVGPCPECDGSMFLISFKDNRFLGCTKR CGYTHSVPKTGKLTLLDKTCETCGWKLFRLKEEVEGNESLEEDICINRRCTEGIKYWKKVGPRSGARAQGKISADSGAEL GARSTARNKERTTARRVSKTVSVADSRKDRK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=670, Percent_Identity=32.2388059701493, Blast_Score=324, Evalue=2e-88, Organism=Homo sapiens, GI4507635, Length=685, Percent_Identity=31.8248175182482, Blast_Score=308, Evalue=2e-83, Organism=Escherichia coli, GI1787529, Length=688, Percent_Identity=24.2732558139535, Blast_Score=168, Evalue=2e-42, Organism=Escherichia coli, GI1788061, Length=612, Percent_Identity=24.8366013071895, Blast_Score=139, Evalue=1e-33, Organism=Caenorhabditis elegans, GI32563869, Length=761, Percent_Identity=30.7490144546649, Blast_Score=315, Evalue=6e-86, Organism=Caenorhabditis elegans, GI17555378, Length=580, Percent_Identity=32.9310344827586, Blast_Score=283, Evalue=3e-76, Organism=Saccharomyces cerevisiae, GI6323263, Length=593, Percent_Identity=31.5345699831366, Blast_Score=231, Evalue=3e-61, Organism=Drosophila melanogaster, GI24640096, Length=784, Percent_Identity=29.5918367346939, Blast_Score=321, Evalue=2e-87, Organism=Drosophila melanogaster, GI24585251, Length=688, Percent_Identity=31.9767441860465, Blast_Score=315, Evalue=1e-85,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005739 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 92467; Mature: 92335
Theoretical pI: Translated: 8.59; Mature: 8.59
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.3 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 2.3 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTVVAFAEKNKAAAQIASILGEGEVDKISVEGLPAYEFKWKGEEWLVMGLSGHIMNYDFP CEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEEEECCCCEECCCCH EKYNKWSEVDPGTLLGVDPEKFVTRKEYEAAIKNLAKRADKIILACDYDREGENIGFEAK HHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHH TLAEEVTDVPVARARFSALSPKEVRKAFENPVDPDHNLAMAAEARQILDLKMGAAFTRFV HHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCEEHHE TLSVREKARTKDVLSIGPCQTPTCGFVYEREKAIRAFEAKDFWKITAVFAAKGGEFEGTH EEEHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCC RAGNIHDKEKAAEIFKRLKGAKEGLVVKKTVKEAKTSPPNPLNTTEFLKRASKFLGISPE CCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHH VALETAEQLYLAGFTSYPRTETNKYADDFDFKSLVFDFARQKEYKPFAESILSAPIVPKN HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC GEKDAHDHPPIHPIRAAARGEVSSAVNIPQAAEVYDLIARHFLANLMPAAVFEKTHLHLQ CCCCCCCCCCCCHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE VKEEPFDSSGTVLKEPGWLEVYPFENKKDKLLPFVEEKERVDVKKLSNTKSKTSPPKKLT EECCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH EAELLTLMDRHGIGTKATAPTHIETNKKRGYFENKGKTISILDTGFTLMEGLSLSVPILV HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCEEE KPDIRSKIEALIQEVEDGKKEFEAALADGTVLIKEMYAQLEANKKELTSNIAGTIKDEAA CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHH LEDKKNYIGTCKACGHVLRIIQTDSGRFVGCTGYPDCRNSFSLPKTGALTVLRSKECKKE HHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHC GAAVLKVGNKYNWAVGIGPCFKCEFEKECYPPETVGPCPECDGSMFLISFKDNRFLGCTK CCEEEEECCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCHH RCGYTHSVPKTGKLTLLDKTCETCGWKLFRLKEEVEGNESLEEDICINRRCTEGIKYWKK HCCCCCCCCCCCCEEEEHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHH VGPRSGARAQGKISADSGAELGARSTARNKERTTARRVSKTVSVADSRKDRK CCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCC >Mature Secondary Structure TVVAFAEKNKAAAQIASILGEGEVDKISVEGLPAYEFKWKGEEWLVMGLSGHIMNYDFP EEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEECCCEEEEEECCCCEECCCCH EKYNKWSEVDPGTLLGVDPEKFVTRKEYEAAIKNLAKRADKIILACDYDREGENIGFEAK HHHCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHH TLAEEVTDVPVARARFSALSPKEVRKAFENPVDPDHNLAMAAEARQILDLKMGAAFTRFV HHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCEEHHE TLSVREKARTKDVLSIGPCQTPTCGFVYEREKAIRAFEAKDFWKITAVFAAKGGEFEGTH EEEHHHHHHHCCCEEECCCCCCCCCCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCC RAGNIHDKEKAAEIFKRLKGAKEGLVVKKTVKEAKTSPPNPLNTTEFLKRASKFLGISPE CCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHH VALETAEQLYLAGFTSYPRTETNKYADDFDFKSLVFDFARQKEYKPFAESILSAPIVPKN HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC GEKDAHDHPPIHPIRAAARGEVSSAVNIPQAAEVYDLIARHFLANLMPAAVFEKTHLHLQ CCCCCCCCCCCCHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE VKEEPFDSSGTVLKEPGWLEVYPFENKKDKLLPFVEEKERVDVKKLSNTKSKTSPPKKLT EECCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH EAELLTLMDRHGIGTKATAPTHIETNKKRGYFENKGKTISILDTGFTLMEGLSLSVPILV HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCCCCEEE KPDIRSKIEALIQEVEDGKKEFEAALADGTVLIKEMYAQLEANKKELTSNIAGTIKDEAA CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHH LEDKKNYIGTCKACGHVLRIIQTDSGRFVGCTGYPDCRNSFSLPKTGALTVLRSKECKKE HHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHC GAAVLKVGNKYNWAVGIGPCFKCEFEKECYPPETVGPCPECDGSMFLISFKDNRFLGCTK CCEEEEECCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEECCHH RCGYTHSVPKTGKLTLLDKTCETCGWKLFRLKEEVEGNESLEEDICINRRCTEGIKYWKK HCCCCCCCCCCCCEEEEHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHH VGPRSGARAQGKISADSGAELGARSTARNKERTTARRVSKTVSVADSRKDRK CCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9389475 [H]