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The map label for this gene is mptN

Identifier: 21226213

GI number: 21226213

Start: 137429

End: 138403

Strand: Direct

Name: mptN

Synonym: MM_0111

Alternate gene names: 21226213

Gene position: 137429-138403 (Clockwise)

Preceding gene: 21226212

Following gene: 21226214

Centisome position: 3.35

GC content: 44.72

Gene sequence:

>975_bases
ATGAAAAAAATAGGGATTGCAATTACTGATCCTGAAGACTGGACAGCCCGGGCACTCATCAAAGCGGCAAAAGAAAAAGG
TTTTTCCCCTCTTGTTTTTGACCTCAGTGATGCGGAAGTCAGGATAGGTTCAGGCATTTCTGAGCCAGCCTCCATTTTTA
AAGCAGGTGAAGTTCTGCTCTCAGACCTTGATGCGCTTATTGTAAGGGATGTGGGTGCAGGGGCTTTTGAAGGAGTTTCA
TTCAGGTTTGACATACTCAGGGAACTCGAGGCAGAAGGGGTTTCGGTTATAAACTCTCCAGAAGCCATCCAGAATGCGGC
AAACAAATACCATGCTTCTTATCTTCTTGCAAAAGCCGGGCTTCCTGTGCCTGAAACTGTAGCTGTCCAGAGTCTTGAGG
CAGCATTAAAAGTAATATCAGAATTCGGGGACGCTGTTATAAAACCTGTTTTTGGATATAAAGGGAAGGATATTGCAAGG
GTAAAGAACCGGGAAATCCGGTTTTCAGACCGAAAGATTGAGCCTGCACCTGTTGAGGAAATTCTCGAAAAGCTGCTTGA
AGAGAAAGGGATGCTCTATATTCAGGAATTTATAGAAAACCCGGGGAGAGACATAAGGGCTTTTGTTGTGGGCGGCACTG
CCATAGGAGCTATTTATAGAAAAGCTGCTGCTGGCTCCTGGGTGAACAACCTGAGCCAGGGAGGAAGTTCGGATAGATGC
GTTCTTACCGATGAGCAGGAAAAAATTGCCGAAAAAGCTTCACTTGCGCTTGGAACGACATTTGCAGGCATTGATATTAT
AGAAGGCACGGAAGAATCGGAAGAAAACAAAAAAACCGAAGGCATGAGCAGTGAAAACAGGAAAAGTCCAAGGATACTTG
AAGTCAACGGAACACCTTCAGGAAAAGGGATTTTTGATGCCTGGGGAATAAATCCCGCAGACCACATCCTGGAATACCTT
AAAAATATTTTATGA

Upstream 100 bases:

>100_bases
TGATTACCTTTCCGAACTCTTTTAGCCCCGGATATTTCAATTGAAAATATGATTTAACCCATATCGCAAACTTGATTATA
AAAGTATCAGATCATACCCT

Downstream 100 bases:

>100_bases
ATTAACTATAAATCTGATTGGAGTTAGTATAACATATTTTAGAGAGAGGATTCATAAAGGAATTATCAAACCCGTAGACT
GCTGATTCAGCCGGCGGACC

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKKIGIAITDPEDWTARALIKAAKEKGFSPLVFDLSDAEVRIGSGISEPASIFKAGEVLLSDLDALIVRDVGAGAFEGVS
FRFDILRELEAEGVSVINSPEAIQNAANKYHASYLLAKAGLPVPETVAVQSLEAALKVISEFGDAVIKPVFGYKGKDIAR
VKNREIRFSDRKIEPAPVEEILEKLLEEKGMLYIQEFIENPGRDIRAFVVGGTAIGAIYRKAAAGSWVNNLSQGGSSDRC
VLTDEQEKIAEKASLALGTTFAGIDIIEGTEESEENKKTEGMSSENRKSPRILEVNGTPSGKGIFDAWGINPADHILEYL
KNIL

Sequences:

>Translated_324_residues
MKKIGIAITDPEDWTARALIKAAKEKGFSPLVFDLSDAEVRIGSGISEPASIFKAGEVLLSDLDALIVRDVGAGAFEGVS
FRFDILRELEAEGVSVINSPEAIQNAANKYHASYLLAKAGLPVPETVAVQSLEAALKVISEFGDAVIKPVFGYKGKDIAR
VKNREIRFSDRKIEPAPVEEILEKLLEEKGMLYIQEFIENPGRDIRAFVVGGTAIGAIYRKAAAGSWVNNLSQGGSSDRC
VLTDEQEKIAEKASLALGTTFAGIDIIEGTEESEENKKTEGMSSENRKSPRILEVNGTPSGKGIFDAWGINPADHILEYL
KNIL
>Mature_324_residues
MKKIGIAITDPEDWTARALIKAAKEKGFSPLVFDLSDAEVRIGSGISEPASIFKAGEVLLSDLDALIVRDVGAGAFEGVS
FRFDILRELEAEGVSVINSPEAIQNAANKYHASYLLAKAGLPVPETVAVQSLEAALKVISEFGDAVIKPVFGYKGKDIAR
VKNREIRFSDRKIEPAPVEEILEKLLEEKGMLYIQEFIENPGRDIRAFVVGGTAIGAIYRKAAAGSWVNNLSQGGSSDRC
VLTDEQEKIAEKASLALGTTFAGIDIIEGTEESEENKKTEGMSSENRKSPRILEVNGTPSGKGIFDAWGINPADHILEYL
KNIL

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=187, Percent_Identity=30.4812834224599, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI134254462, Length=188, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787076, Length=323, Percent_Identity=26.9349845201238, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MPTN_METMA (Q8Q0M5)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632135.1
- ProteinModelPortal:   Q8Q0M5
- GeneID:   1478453
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0111
- NMPDR:   fig|192952.1.peg.111
- HOGENOM:   HBG679591
- OMA:   VMRTLLI
- ProtClustDB:   CLSK568978
- BioCyc:   MMAZ192952:MM0111-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 35037; Mature: 35037

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGIAITDPEDWTARALIKAAKEKGFSPLVFDLSDAEVRIGSGISEPASIFKAGEVLL
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHH
SDLDALIVRDVGAGAFEGVSFRFDILRELEAEGVSVINSPEAIQNAANKYHASYLLAKAG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCC
LPVPETVAVQSLEAALKVISEFGDAVIKPVFGYKGKDIARVKNREIRFSDRKIEPAPVEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCHHH
ILEKLLEEKGMLYIQEFIENPGRDIRAFVVGGTAIGAIYRKAAAGSWVNNLSQGGSSDRC
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCEE
VLTDEQEKIAEKASLALGTTFAGIDIIEGTEESEENKKTEGMSSENRKSPRILEVNGTPS
EECCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCC
GKGIFDAWGINPADHILEYLKNIL
CCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGIAITDPEDWTARALIKAAKEKGFSPLVFDLSDAEVRIGSGISEPASIFKAGEVLL
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHH
SDLDALIVRDVGAGAFEGVSFRFDILRELEAEGVSVINSPEAIQNAANKYHASYLLAKAG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCC
LPVPETVAVQSLEAALKVISEFGDAVIKPVFGYKGKDIARVKNREIRFSDRKIEPAPVEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCHHH
ILEKLLEEKGMLYIQEFIENPGRDIRAFVVGGTAIGAIYRKAAAGSWVNNLSQGGSSDRC
HHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHCCCCCCEE
VLTDEQEKIAEKASLALGTTFAGIDIIEGTEESEENKKTEGMSSENRKSPRILEVNGTPS
EECCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCC
GKGIFDAWGINPADHILEYLKNIL
CCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824