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The map label for this gene is cobQ [H]

Identifier: 21226195

GI number: 21226195

Start: 120209

End: 121708

Strand: Direct

Name: cobQ [H]

Synonym: MM_0093

Alternate gene names: 21226195

Gene position: 120209-121708 (Clockwise)

Preceding gene: 21226181

Following gene: 21226197

Centisome position: 2.93

GC content: 46.8

Gene sequence:

>1500_bases
TTGAATAATAAAAAGCCTGTTTGCGCAGAAGGTATGGTAAAAATGGAGAAAAAAAGCCTATTGATCCTGGGAACCGCCTC
TCATGTTGGAAAAAGTTCAGTCGTAACTGCCATATGCAGGATCCTGTCCAGAAGCTACAGGGTTGCTCCTTTCAAGGCTC
AGAATATGAGCCTGAACTCCTGGATTACAAAGGACGGAAAGGAGATAGGGATTGCACAGGCAATTCAGGCAAAAGCCGCA
GGCACTGAACCCACTGCAGATATGAACCCTGTTCTCCTGAAACCAAAAGGGGACTGCGTTTCCCAGGTAGTCCTGCTGGG
CGAGCCCTATGCTGACAAAAGTGCAGGCCAGTATTACGATTCCATTGAAGAGATGCACGCAGTGCTTAAAGGAGCCCTTG
AAAGGCTCTGGAAAGAGCATGACATTATTGTTATGGAAGGAGCCGGGGGAGCTGCAGAAATCAACCTTTATGAGAGGGAC
ATCGTGAATGTGGGTACTGCAAGGCTCACAAAGGCTCCTATAATCCTTGTAGGAGATATTGAAAGGGGAGGCGTGTTTGC
AAGCCTTTACGGCACTATTGCCCTTCTGCCTGAAGATGTAAGGAAAAACGTCAAAGGGTTTATAATCAACAAGTTCAGGG
GCGACATAGAAATCCTGAGACCCGGACTCAAGCAGCTTGAGGATAAAACCGGAATCCCTGTGCTTGGGGTCCTTCCTCAT
TTCAAGCTCCGCATCCCTTCCGAAGATTCGGTCTCTCTCGGAGATAAAGAGGATTCAAAGGCAGAAAAAGAAATAGAGGT
TGCAGTAATCCGCTTGCCAAGGATCTCAAACTTTACGGACTTCGAACCCCTTGAGGGGCTCGTAAAAGTTAGGTATGTGG
ACATCAACGAAGAACTCGGAAACCCTGATGCAATCATGATTCCTGGGACGAAAAATACTGTTAATGACCTTCTTGACCTC
AGGGCAAGCGGGATGGACAAAAAAATTCAGGCTTTCAAAGGAAAAGTCCCTATCTTCGGGATCTGTGGCGGTTACCAGAT
GCTTGGCAGGACTATCTTTGATTCCGGAGTAGAGAACGGAGTCGAAGCCGAGTTTGAAGGCCTGGGGCTTCTGGACATAG
GGACCAGATTCGGAGAATATAAAAAGAGAACTGTCCAGGTAACAAAAAAAGTCAATGGTTACGGCCCTATCCTGAGACCC
ATAGATGGTGAAGAGATAAAAGGTTATGAGATCCATATGGGAGTTACGGATTCAAACCGCACCGTTTTCGGAGACGATGG
AGCTATTGATGAGGACGGTTTCGTAATCGGCACATACCTTCACGGGCTTTTTGATAACAGGAATATAAGAAATGCCCTTG
TCCGGTATCTTTATGAGAAGAAGGGCCTGGAATACGAACCAGATGAGGCAATAAGCGAAAACGATGCTTACGAAGAGCTC
GCAAATGTGGTTGAGCAGAACATTGATATGGAAAAGCTCTTCGAGATCGCAGGAGTCTGA

Upstream 100 bases:

>100_bases
ACAATTATTAAATTTAAACAAGTTTACTTGATTTATATCTGCAGAAGCAAAAATCTTACTTTGATTTTAATAAATTGTCA
GTTAAAACCATTACTTGTAT

Downstream 100 bases:

>100_bases
AACAGACACGCAGAGAAATAAAGCATAAAAAACAGTTTTTGAGCAGGTTCTGCGGAAGTATGGGGAATAGCTGGAATAAA
AAATGGAACCTAGGGTAAAG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MNNKKPVCAEGMVKMEKKSLLILGTASHVGKSSVVTAICRILSRSYRVAPFKAQNMSLNSWITKDGKEIGIAQAIQAKAA
GTEPTADMNPVLLKPKGDCVSQVVLLGEPYADKSAGQYYDSIEEMHAVLKGALERLWKEHDIIVMEGAGGAAEINLYERD
IVNVGTARLTKAPIILVGDIERGGVFASLYGTIALLPEDVRKNVKGFIINKFRGDIEILRPGLKQLEDKTGIPVLGVLPH
FKLRIPSEDSVSLGDKEDSKAEKEIEVAVIRLPRISNFTDFEPLEGLVKVRYVDINEELGNPDAIMIPGTKNTVNDLLDL
RASGMDKKIQAFKGKVPIFGICGGYQMLGRTIFDSGVENGVEAEFEGLGLLDIGTRFGEYKKRTVQVTKKVNGYGPILRP
IDGEEIKGYEIHMGVTDSNRTVFGDDGAIDEDGFVIGTYLHGLFDNRNIRNALVRYLYEKKGLEYEPDEAISENDAYEEL
ANVVEQNIDMEKLFEIAGV

Sequences:

>Translated_499_residues
MNNKKPVCAEGMVKMEKKSLLILGTASHVGKSSVVTAICRILSRSYRVAPFKAQNMSLNSWITKDGKEIGIAQAIQAKAA
GTEPTADMNPVLLKPKGDCVSQVVLLGEPYADKSAGQYYDSIEEMHAVLKGALERLWKEHDIIVMEGAGGAAEINLYERD
IVNVGTARLTKAPIILVGDIERGGVFASLYGTIALLPEDVRKNVKGFIINKFRGDIEILRPGLKQLEDKTGIPVLGVLPH
FKLRIPSEDSVSLGDKEDSKAEKEIEVAVIRLPRISNFTDFEPLEGLVKVRYVDINEELGNPDAIMIPGTKNTVNDLLDL
RASGMDKKIQAFKGKVPIFGICGGYQMLGRTIFDSGVENGVEAEFEGLGLLDIGTRFGEYKKRTVQVTKKVNGYGPILRP
IDGEEIKGYEIHMGVTDSNRTVFGDDGAIDEDGFVIGTYLHGLFDNRNIRNALVRYLYEKKGLEYEPDEAISENDAYEEL
ANVVEQNIDMEKLFEIAGV
>Mature_499_residues
MNNKKPVCAEGMVKMEKKSLLILGTASHVGKSSVVTAICRILSRSYRVAPFKAQNMSLNSWITKDGKEIGIAQAIQAKAA
GTEPTADMNPVLLKPKGDCVSQVVLLGEPYADKSAGQYYDSIEEMHAVLKGALERLWKEHDIIVMEGAGGAAEINLYERD
IVNVGTARLTKAPIILVGDIERGGVFASLYGTIALLPEDVRKNVKGFIINKFRGDIEILRPGLKQLEDKTGIPVLGVLPH
FKLRIPSEDSVSLGDKEDSKAEKEIEVAVIRLPRISNFTDFEPLEGLVKVRYVDINEELGNPDAIMIPGTKNTVNDLLDL
RASGMDKKIQAFKGKVPIFGICGGYQMLGRTIFDSGVENGVEAEFEGLGLLDIGTRFGEYKKRTVQVTKKVNGYGPILRP
IDGEEIKGYEIHMGVTDSNRTVFGDDGAIDEDGFVIGTYLHGLFDNRNIRNALVRYLYEKKGLEYEPDEAISENDAYEEL
ANVVEQNIDMEKLFEIAGV

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 54856; Mature: 54856

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKKPVCAEGMVKMEKKSLLILGTASHVGKSSVVTAICRILSRSYRVAPFKAQNMSLNS
CCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEECCEECCCCCHHH
WITKDGKEIGIAQAIQAKAAGTEPTADMNPVLLKPKGDCVSQVVLLGEPYADKSAGQYYD
HCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHEECCCCCCCCCHHHHH
SIEEMHAVLKGALERLWKEHDIIVMEGAGGAAEINLYERDIVNVGTARLTKAPIILVGDI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHHCCCHHHCCCCEEEEECC
ERGGVFASLYGTIALLPEDVRKNVKGFIINKFRGDIEILRPGLKQLEDKTGIPVLGVLPH
CCCCEEEEEHHHHEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
FKLRIPSEDSVSLGDKEDSKAEKEIEVAVIRLPRISNFTDFEPLEGLVKVRYVDINEELG
EEEECCCCCCCCCCCCCCCHHHHEEEEEEEEECCCCCCCCCCHHCCEEEEEEEECHHHCC
NPDAIMIPGTKNTVNDLLDLRASGMDKKIQAFKGKVPIFGICGGYQMLGRTIFDSGVENG
CCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCC
VEAEFEGLGLLDIGTRFGEYKKRTVQVTKKVNGYGPILRPIDGEEIKGYEIHMGVTDSNR
CCCHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCC
TVFGDDGAIDEDGFVIGTYLHGLFDNRNIRNALVRYLYEKKGLEYEPDEAISENDAYEEL
EEECCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHH
ANVVEQNIDMEKLFEIAGV
HHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MNNKKPVCAEGMVKMEKKSLLILGTASHVGKSSVVTAICRILSRSYRVAPFKAQNMSLNS
CCCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEECCEECCCCCHHH
WITKDGKEIGIAQAIQAKAAGTEPTADMNPVLLKPKGDCVSQVVLLGEPYADKSAGQYYD
HCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHEECCCCCCCCCHHHHH
SIEEMHAVLKGALERLWKEHDIIVMEGAGGAAEINLYERDIVNVGTARLTKAPIILVGDI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEHHHHHHCCCHHHCCCCEEEEECC
ERGGVFASLYGTIALLPEDVRKNVKGFIINKFRGDIEILRPGLKQLEDKTGIPVLGVLPH
CCCCEEEEEHHHHEECCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCC
FKLRIPSEDSVSLGDKEDSKAEKEIEVAVIRLPRISNFTDFEPLEGLVKVRYVDINEELG
EEEECCCCCCCCCCCCCCCHHHHEEEEEEEEECCCCCCCCCCHHCCEEEEEEEECHHHCC
NPDAIMIPGTKNTVNDLLDLRASGMDKKIQAFKGKVPIFGICGGYQMLGRTIFDSGVENG
CCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCC
VEAEFEGLGLLDIGTRFGEYKKRTVQVTKKVNGYGPILRPIDGEEIKGYEIHMGVTDSNR
CCCHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCC
TVFGDDGAIDEDGFVIGTYLHGLFDNRNIRNALVRYLYEKKGLEYEPDEAISENDAYEEL
EEECCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHH
ANVVEQNIDMEKLFEIAGV
HHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]