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The map label for this gene is ybiK [C]

Identifier: 212224543

GI number: 212224543

Start: 1265828

End: 1266748

Strand: Reverse

Name: ybiK [C]

Synonym: TON_1392

Alternate gene names: 212224543

Gene position: 1266748-1265828 (Counterclockwise)

Preceding gene: 212224544

Following gene: 212224542

Centisome position: 68.56

GC content: 54.72

Gene sequence:

>921_bases
ATGGTCGCGATAATAGTTCACGGTGGTGCTGGTACCATAAGGAAGGAGGAACGTATTCCAAAGGTTATTGAAGGTGTTAG
AGAGGCTGTTCTTGCCGGCTGGCGCGAGCTGAAGAGGGGTTCGGCTTTGGATGCCGTTGAGGAGGCCCTCAAAGCTCTTG
AGGATAACCCTATCTTCAACGCCGGAACTGGTTCCGTCCTGACCCTCGACGGCAAGGTCGAGATGGATGCGGCAATAATG
CGCGGCAAAACCCTTGAAGCCGGTGCAGTTGCCGGGATATGGGGCGTTAAGAACCCCATAAGCGTTGCGAGGAAGGTAAT
GGAGAAGACCGATCACGTTATCCTCAGTGGAGAGGGTGCTGTTAAGTTCGCCCGGTTGATGGGCTTTGAAGAGTACGACC
CGAGGACAGAGGAGAGACTCAAGCAGTGGGAAGAGCTCAGGAAGAAGCTCCTTGAAACTGGAGAAGTAAAGCACTGGAAG
AAGCTGAGTGAGTTGATTAAGGAGTATCCCGAAGTCCTGAGGAGCACGGTTGGTGCGGTTGCCTTCGACGGCGAGGAGGT
TGTGGCTGGTACATCAACCGGCGGCGTTTTCCTCAAGATGTTCGGCCGCGTTGGTGATACACCGATAATCGGAGGCGGAA
CCTACGCTAACGAAGTTGCCGGGGCTTCCTGTACTGGCCTCGGTGAGGTCGCTATAAAGCTGGCTCTGGCCAAGAGCGCG
ACCGACTTCGTCCGCTTGGGAATGGATGCTCAGGCTGCCAGCGAGGCCGCGATAAGCCTTGCCACAAGGTACTTTGGCCC
CGATACCATGGGCATCATAATGGTTGATTCCCGCGGAAACATTGGCTTCGCCAAGAACACCAAACACATGAGCTACGCTT
TCATGAAAGAAGGAATGGAAGAACCTCACGCAGGTGTCTGA

Upstream 100 bases:

>100_bases
AGGTGAAACTATAATCAACATGGCGCTGAGCTGAAGTTCCAATCTTTTCGTTTCCACAACTCTTTTAACCATTCGAGATT
CATTGCCTACGGTGATTCTC

Downstream 100 bases:

>100_bases
ATGGGAAAGTGGGATATCTGGTTCCTCCACGCTTCAACGTTTTTCTTCTTCCTTGGAATGGCGGTAGTCAATCCTCTAAT
CTCCCCCCTAGCTATGACCC

Product: L-asparaginase

Products: NA

Alternate protein names: L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEALKALEDNPIFNAGTGSVLTLDGKVEMDAAIM
RGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVILSGEGAVKFARLMGFEEYDPRTEERLKQWEELRKKLLETGEVKHWK
KLSELIKEYPEVLRSTVGAVAFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSA
TDFVRLGMDAQAASEAAISLATRYFGPDTMGIIMVDSRGNIGFAKNTKHMSYAFMKEGMEEPHAGV

Sequences:

>Translated_306_residues
MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEALKALEDNPIFNAGTGSVLTLDGKVEMDAAIM
RGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVILSGEGAVKFARLMGFEEYDPRTEERLKQWEELRKKLLETGEVKHWK
KLSELIKEYPEVLRSTVGAVAFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSA
TDFVRLGMDAQAASEAAISLATRYFGPDTMGIIMVDSRGNIGFAKNTKHMSYAFMKEGMEEPHAGV
>Mature_306_residues
MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEALKALEDNPIFNAGTGSVLTLDGKVEMDAAIM
RGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVILSGEGAVKFARLMGFEEYDPRTEERLKQWEELRKKLLETGEVKHWK
KLSELIKEYPEVLRSTVGAVAFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSA
TDFVRLGMDAQAASEAAISLATRYFGPDTMGIIMVDSRGNIGFAKNTKHMSYAFMKEGMEEPHAGV

Specific function: Unknown

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=300, Percent_Identity=41, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI145275200, Length=300, Percent_Identity=41, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI285002251, Length=319, Percent_Identity=31.3479623824451, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI98991769, Length=270, Percent_Identity=33.7037037037037, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI285002253, Length=310, Percent_Identity=29.3548387096774, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1787050, Length=316, Percent_Identity=38.9240506329114, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17569239, Length=243, Percent_Identity=30.4526748971193, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564649, Length=181, Percent_Identity=33.7016574585635, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI18921183, Length=313, Percent_Identity=36.4217252396166, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI21357893, Length=261, Percent_Identity=36.7816091954023, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI221330102, Length=307, Percent_Identity=31.5960912052117, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24652135, Length=292, Percent_Identity=32.5342465753425, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24657358, Length=277, Percent_Identity=32.4909747292419, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24655553, Length=260, Percent_Identity=26.5384615384615, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32799; Mature: 32799

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEALKALEDNPIFN
CEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
AGTGSVLTLDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVILSGEGA
CCCCCEEEECCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCH
VKFARLMGFEEYDPRTEERLKQWEELRKKLLETGEVKHWKKLSELIKEYPEVLRSTVGAV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
AFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSA
EECCCEEEECCCCCHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
TDFVRLGMDAQAASEAAISLATRYFGPDTMGIIMVDSRGNIGFAKNTKHMSYAFMKEGME
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCC
EPHAGV
CCCCCC
>Mature Secondary Structure
MVAIIVHGGAGTIRKEERIPKVIEGVREAVLAGWRELKRGSALDAVEEALKALEDNPIFN
CEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
AGTGSVLTLDGKVEMDAAIMRGKTLEAGAVAGIWGVKNPISVARKVMEKTDHVILSGEGA
CCCCCEEEECCCCCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCH
VKFARLMGFEEYDPRTEERLKQWEELRKKLLETGEVKHWKKLSELIKEYPEVLRSTVGAV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHE
AFDGEEVVAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAIKLALAKSA
EECCCEEEECCCCCHHHHHHHHCCCCCCEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHH
TDFVRLGMDAQAASEAAISLATRYFGPDTMGIIMVDSRGNIGFAKNTKHMSYAFMKEGME
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCC
EPHAGV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA