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The map label for this gene is dphB

Identifier: 212224523

GI number: 212224523

Start: 1240974

End: 1241768

Strand: Reverse

Name: dphB

Synonym: TON_1372

Alternate gene names: 212224523

Gene position: 1241768-1240974 (Counterclockwise)

Preceding gene: 212224525

Following gene: 212224521

Centisome position: 67.21

GC content: 54.09

Gene sequence:

>795_bases
ATGGCGATATACTTCATTGGGTTGGGACTCTACGACGAAAAAGACATCACGCTCAAGGGCCTGGAGACGGCCAGAAAATG
CGACCTCGTCTTTGCCGAGTTCTACACCTCGCTTTTGGCCGGGACGACGCTTGATAAGATTGAGGAGCTCATAGGGAAGC
CCATCAGGAGGCTCAGCAGGGAAGAGGTCGAGCTCCAATTTGAGAGAATCGTTCTTAGCGAGGCGAAGGGCAAGGACGTC
GCCTTTCTAACCGCGGGTGACCCTATGGTGGCAACCACTCACTCCGACCTCCGCATAAGGGCGAAGGAGATGGGAATCGA
GAGTTACGTCATCCACGCGCCAAGCATCTATTCCGCCATAGCAATAACCGGCCTTCAGGTATACAAGTTCGGCAAGAGCG
CAACCGTAGCTTACCCAGAGAAAAACTGGTTTCCAACGAGTCACTACGACGTGATAAGAGAGAACAAAGAGCGGGGGCTT
CACACGATGCTCTTCCTTGACATAAAGGCTGATCAGAATCGCTACATGACAGCTAACGAGGCTATGGAGATTCTCCTCCA
GGTCGAGGAGATGAAGGGGGAAGGTGTTTTCACGCCTGATACGCTGGTAGTCGTTCTTGCGCGTGCGGGCTCGCTCAATC
CGACGCTCAAAGCTGGCTACGTTAGGGACATGCTCAACGAGGACTTCGGCAGACAGCCGCACGTTATGGTCGTTCCGGGG
AGACTGCACATTGTTGAGGCGGAATACTTAGTGACCTTCGCCGGTGCACCGAGGAAGATACTCGACGAGGTCTAG

Upstream 100 bases:

>100_bases
GGTTTAATCAGTAATTGAGAGATTAAATGAAGTTTCAGAGTTTCCCACATTGGCGGTCAAGCTTAAAAACCCCCCCTCCT
ACGCTCGGTCGGTGGGGAAA

Downstream 100 bases:

>100_bases
CGAAAGCTTTATATTTTCGTTCCCTTCCACTATTCCCGGGACGGGCCCGTGGTCTAGCCTGGTTATGACGCCGCCCTCAC
ACGGCGGAGGTCCGGGGTTC

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAIYFIGLGLYDEKDITLKGLETARKCDLVFAEFYTSLLAGTTLDKIEELIGKPIRRLSREEVELQFERIVLSEAKGKDV
AFLTAGDPMVATTHSDLRIRAKEMGIESYVIHAPSIYSAIAITGLQVYKFGKSATVAYPEKNWFPTSHYDVIRENKERGL
HTMLFLDIKADQNRYMTANEAMEILLQVEEMKGEGVFTPDTLVVVLARAGSLNPTLKAGYVRDMLNEDFGRQPHVMVVPG
RLHIVEAEYLVTFAGAPRKILDEV

Sequences:

>Translated_264_residues
MAIYFIGLGLYDEKDITLKGLETARKCDLVFAEFYTSLLAGTTLDKIEELIGKPIRRLSREEVELQFERIVLSEAKGKDV
AFLTAGDPMVATTHSDLRIRAKEMGIESYVIHAPSIYSAIAITGLQVYKFGKSATVAYPEKNWFPTSHYDVIRENKERGL
HTMLFLDIKADQNRYMTANEAMEILLQVEEMKGEGVFTPDTLVVVLARAGSLNPTLKAGYVRDMLNEDFGRQPHVMVVPG
RLHIVEAEYLVTFAGAPRKILDEV
>Mature_263_residues
AIYFIGLGLYDEKDITLKGLETARKCDLVFAEFYTSLLAGTTLDKIEELIGKPIRRLSREEVELQFERIVLSEAKGKDVA
FLTAGDPMVATTHSDLRIRAKEMGIESYVIHAPSIYSAIAITGLQVYKFGKSATVAYPEKNWFPTSHYDVIRENKERGLH
TMLFLDIKADQNRYMTANEAMEILLQVEEMKGEGVFTPDTLVVVLARAGSLNPTLKAGYVRDMLNEDFGRQPHVMVVPGR
LHIVEAEYLVTFAGAPRKILDEV

Specific function: Required for the methylation step in diphthamide biosynthesis

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family

Homologues:

Organism=Homo sapiens, GI117190324, Length=271, Percent_Identity=39.8523985239852, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI117190326, Length=272, Percent_Identity=39.7058823529412, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI117190262, Length=272, Percent_Identity=39.7058823529412, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17531369, Length=273, Percent_Identity=37.3626373626374, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323201, Length=278, Percent_Identity=39.9280575539568, Blast_Score=192, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28572120, Length=271, Percent_Identity=38.3763837638376, Blast_Score=177, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPHB_THEON (B6YXP9)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307759.1
- ProteinModelPortal:   B6YXP9
- GeneID:   7018403
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_1372
- HOGENOM:   HBG460511
- OMA:   YAEFYTA
- ProtClustDB:   PRK04160
- HAMAP:   MF_01084
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- PANTHER:   PTHR10882
- PIRSF:   PIRSF036432
- TIGRFAMs:   TIGR00522

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.98

Molecular weight: Translated: 29664; Mature: 29533

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIYFIGLGLYDEKDITLKGLETARKCDLVFAEFYTSLLAGTTLDKIEELIGKPIRRLSR
CEEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EEVELQFERIVLSEAKGKDVAFLTAGDPMVATTHSDLRIRAKEMGIESYVIHAPSIYSAI
HHHHHHHHHHHEECCCCCEEEEEECCCCEEEECCCCCEEEHHHCCCCEEEEECCHHHHHH
AITGLQVYKFGKSATVAYPEKNWFPTSHYDVIRENKERGLHTMLFLDIKADQNRYMTANE
HHHCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCHHCCCEEEEEEEEECCCCCEEEHHH
AMEILLQVEEMKGEGVFTPDTLVVVLARAGSLNPTLKAGYVRDMLNEDFGRQPHVMVVPG
HHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECC
RLHIVEAEYLVTFAGAPRKILDEV
EEEEEEEEEEEEECCCCHHHHHCC
>Mature Secondary Structure 
AIYFIGLGLYDEKDITLKGLETARKCDLVFAEFYTSLLAGTTLDKIEELIGKPIRRLSR
EEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EEVELQFERIVLSEAKGKDVAFLTAGDPMVATTHSDLRIRAKEMGIESYVIHAPSIYSAI
HHHHHHHHHHHEECCCCCEEEEEECCCCEEEECCCCCEEEHHHCCCCEEEEECCHHHHHH
AITGLQVYKFGKSATVAYPEKNWFPTSHYDVIRENKERGLHTMLFLDIKADQNRYMTANE
HHHCEEEEECCCCCEEECCCCCCCCCCHHHHHHCCHHCCCEEEEEEEEECCCCCEEEHHH
AMEILLQVEEMKGEGVFTPDTLVVVLARAGSLNPTLKAGYVRDMLNEDFGRQPHVMVVPG
HHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECC
RLHIVEAEYLVTFAGAPRKILDEV
EEEEEEEEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA