Click here to switch to the map view.

The map label for this gene is thrB

Identifier: 209695973

GI number: 209695973

Start: 2735414

End: 2736370

Strand: Reverse

Name: thrB

Synonym: VSAL_I2555

Alternate gene names: 209695973

Gene position: 2736370-2735414 (Counterclockwise)

Preceding gene: 209695974

Following gene: 209695972

Centisome position: 82.29

GC content: 40.54

Gene sequence:

>957_bases
ATGAGTGTTGTTGTTTATGCTCCGGCATCGATTGGTAATGTAAGTGTTGGGTTTGATGTACTTGGTGCAGCGGTTTCTCC
TATTGATGGTTCGCTATTAGGAGATAGAGTTCTTGTTGAATCCGGTTCTGACGCTTTCACTCTTGCCACATCAGGTCGTT
TTGTTGATAAATTGCCAGAAAATCCGCAAGACAATATTGTTTATGATTGTTGGAAAGTGTTTGCAAGAGAGCTTGAAACT
AAAAACGTAACGTTAAAACCTATTCATATGGTACTAGAAAAAAACATGCCAATTGGTTCTGGTTTAGGCTCTAGTGCTTG
CTCTATTGTTGCTGCACTTGATGCATTAAATCAGTTTCATGATAAACCATTAACTGAAATGGAGCTCCTTGCTCTAATGG
GGGAGATGGAAGGACAGATATCAGGTGGGATTCACTATGATAATGTGGCGCCATGCTATCTTGGTGGTTTGCAATTAATG
GTTGAAGAACTCGGTATTATTAGTCAAGAAGTCCCTTGTTTCGATGAATGGTATTGGGTGATGGCATACCCGGGTATTAA
AGTATCAACGGCGGAAGCTCGTGAAATTCTACCTTCTCAATATCGTCGTCAAGATGTCATTGCTCATGGTCGAAATCTCG
CAGGCTTTATTCATGCGTGTTATTCAAAGCAACCAGAGCTAGCCGCGAAAATGATCAAAGATGTGGTGGCTGAACCTTAC
CGTGAACGCTTATTACCTAATTTTGCTCAAGCTCGCGCTTATGCCGCTTCTGCAGGTGCATTAACAACAGGAATTTCTGG
GTCAGGACCAACTCTATTTAGTATTTGTAAAGATAAAGATATTGCTGAAAGAGTATCTCGTTGGTTGCAAGATAATTACG
TTCAAAATGACGAAGGATTTGTCCATGTTTGTCGTTTAGACAAGCAAGGTTCACAAGTAACAGGAAGTAAATTATGA

Upstream 100 bases:

>100_bases
CCATTGGTATTAAGAGGCTATGGCGCAGGAACAGAAGTAACTGCTGCTGGCGTATTCTCTGATCTTATGCGCACCCTTGG
CTGGAAATTAGGAGTATAAA

Downstream 100 bases:

>100_bases
AGTTGTACAACATAAAAGAAAACGATGAGAAAGTCTCTTTTGGCCAAGCCGTTCGCCAAGGTTTAGGTCGTAATCAAGGA
CTGTTTTTTCCATCTGAACT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGSLLGDRVLVESGSDAFTLATSGRFVDKLPENPQDNIVYDCWKVFARELET
KNVTLKPIHMVLEKNMPIGSGLGSSACSIVAALDALNQFHDKPLTEMELLALMGEMEGQISGGIHYDNVAPCYLGGLQLM
VEELGIISQEVPCFDEWYWVMAYPGIKVSTAEAREILPSQYRRQDVIAHGRNLAGFIHACYSKQPELAAKMIKDVVAEPY
RERLLPNFAQARAYAASAGALTTGISGSGPTLFSICKDKDIAERVSRWLQDNYVQNDEGFVHVCRLDKQGSQVTGSKL

Sequences:

>Translated_318_residues
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGSLLGDRVLVESGSDAFTLATSGRFVDKLPENPQDNIVYDCWKVFARELET
KNVTLKPIHMVLEKNMPIGSGLGSSACSIVAALDALNQFHDKPLTEMELLALMGEMEGQISGGIHYDNVAPCYLGGLQLM
VEELGIISQEVPCFDEWYWVMAYPGIKVSTAEAREILPSQYRRQDVIAHGRNLAGFIHACYSKQPELAAKMIKDVVAEPY
RERLLPNFAQARAYAASAGALTTGISGSGPTLFSICKDKDIAERVSRWLQDNYVQNDEGFVHVCRLDKQGSQVTGSKL
>Mature_317_residues
SVVVYAPASIGNVSVGFDVLGAAVSPIDGSLLGDRVLVESGSDAFTLATSGRFVDKLPENPQDNIVYDCWKVFARELETK
NVTLKPIHMVLEKNMPIGSGLGSSACSIVAALDALNQFHDKPLTEMELLALMGEMEGQISGGIHYDNVAPCYLGGLQLMV
EELGIISQEVPCFDEWYWVMAYPGIKVSTAEAREILPSQYRRQDVIAHGRNLAGFIHACYSKQPELAAKMIKDVVAEPYR
ERLLPNFAQARAYAASAGALTTGISGSGPTLFSICKDKDIAERVSRWLQDNYVQNDEGFVHVCRLDKQGSQVTGSKL

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=311, Percent_Identity=68.1672025723473, Blast_Score=447, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_ALISL (B6EKY7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263903.1
- ProteinModelPortal:   B6EKY7
- GeneID:   6989294
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2555
- HOGENOM:   HBG646290
- OMA:   GSAHADN
- ProtClustDB:   PRK01212
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 34593; Mature: 34462

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVVYAPASIGNVSVGFDVLGAAVSPIDGSLLGDRVLVESGSDAFTLATSGRFVDKLPE
CEEEEECCCCCCCCEECHHHHHHHHCCCCCCHHCCEEEEECCCCEEEEEECCCHHHHCCC
NPQDNIVYDCWKVFARELETKNVTLKPIHMVLEKNMPIGSGLGSSACSIVAALDALNQFH
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DKPLTEMELLALMGEMEGQISGGIHYDNVAPCYLGGLQLMVEELGIISQEVPCFDEWYWV
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCEEE
MAYPGIKVSTAEAREILPSQYRRQDVIAHGRNLAGFIHACYSKQPELAAKMIKDVVAEPY
EECCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RERLLPNFAQARAYAASAGALTTGISGSGPTLFSICKDKDIAERVSRWLQDNYVQNDEGF
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCE
VHVCRLDKQGSQVTGSKL
EEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SVVVYAPASIGNVSVGFDVLGAAVSPIDGSLLGDRVLVESGSDAFTLATSGRFVDKLPE
EEEEECCCCCCCCEECHHHHHHHHCCCCCCHHCCEEEEECCCCEEEEEECCCHHHHCCC
NPQDNIVYDCWKVFARELETKNVTLKPIHMVLEKNMPIGSGLGSSACSIVAALDALNQFH
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DKPLTEMELLALMGEMEGQISGGIHYDNVAPCYLGGLQLMVEELGIISQEVPCFDEWYWV
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCEEE
MAYPGIKVSTAEAREILPSQYRRQDVIAHGRNLAGFIHACYSKQPELAAKMIKDVVAEPY
EECCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RERLLPNFAQARAYAASAGALTTGISGSGPTLFSICKDKDIAERVSRWLQDNYVQNDEGF
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCE
VHVCRLDKQGSQVTGSKL
EEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA