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The map label for this gene is mscS [H]

Identifier: 209695964

GI number: 209695964

Start: 2726695

End: 2727555

Strand: Reverse

Name: mscS [H]

Synonym: VSAL_I2546

Alternate gene names: 209695964

Gene position: 2727555-2726695 (Counterclockwise)

Preceding gene: 209695965

Following gene: 209695963

Centisome position: 82.03

GC content: 38.91

Gene sequence:

>861_bases
ATGGCAGATCAAGGTGCAGTTACTGATTTAGCAACAACTGGCGTTGCTAATTTAGAGCGTTGGCTTACAGATAATTCAGA
ACTCTTTATTACTTATGGTGTAAATATTATTTCGGCACTAGTTATTCTTTTTATCGGTAATATTATCGTTAAGAAAGTCG
CTGGTAGTGTAAGTAAAGTTCTTGAAAAGAAAAAAATGGACAAAGCAGTCGTTGACTTTGCTCATACATTAGTTCGTTAC
CTTTTATTTATTATCGTATTAATTGCAGCATTAGGTAAAGTAGGTGTTCAAACGGCTTCTGTTGTTGCTGTTATTGGTGC
GGCTGGTTTAGCGGTAGGTCTTGCACTTCAAGGTTCGTTATCAAACTTTGCAGCTGGTGTATTGATTGTTGCCTTTCGTC
CATTCAAATCTGGCGATTACGTTGAGATTGGCGGCGTGGCTGGTTCTGTTGAGTCTATCCAAATTTTCCAAACGATCCTA
ACTACTCCAGATAATAAAATGATTGTTGTGCCGAATGGGGGTGTTATTGGTAGCCCAATTACTAACTATTCTCGTCATGC
TACACGTCGTGTTGATCATGTTATTGGCGTTTCTTATAGTGCTGATCTTAAGAAAACTAAAGAAGTGATCACAAAAGTAC
TTGAGGCTGATCCTCGTGTACTTAAAACACCGGCACCAACGGTTGGTGTTGTTGCTTTAGCTGATTCATCAGTAAACTTT
GTGGTTCGTCCGTGGTGTAAAACTGCAGATTACTGGGATGTTTATTTTGATACGCTTCAAGCGATTAAAGAAGGTCTAGA
TAGCGAAGGAATTGAAATCCCATTCCCGCAAATGGATGTTCATTTGAATAAAGCTGATTAG

Upstream 100 bases:

>100_bases
TAACACCACACTGACTATCGATAGTGTTAGAATAGGTATAAATCGTCAAAATAATGACTACATTCTCATTCGCATGAGAA
CTATATAAAGGACAAATCAA

Downstream 100 bases:

>100_bases
CCTTTATGGTCTTAGTGTAGAAAATGCTTCCTTCGGGGAGCATTTTTTTTATCTGCTACTTATCTATTATCCTTTTGGAT
TAGAGTGGACTAGAATGAAT

Product: small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MADQGAVTDLATTGVANLERWLTDNSELFITYGVNIISALVILFIGNIIVKKVAGSVSKVLEKKKMDKAVVDFAHTLVRY
LLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVESIQIFQTIL
TTPDNKMIVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLEADPRVLKTPAPTVGVVALADSSVNF
VVRPWCKTADYWDVYFDTLQAIKEGLDSEGIEIPFPQMDVHLNKAD

Sequences:

>Translated_286_residues
MADQGAVTDLATTGVANLERWLTDNSELFITYGVNIISALVILFIGNIIVKKVAGSVSKVLEKKKMDKAVVDFAHTLVRY
LLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVESIQIFQTIL
TTPDNKMIVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLEADPRVLKTPAPTVGVVALADSSVNF
VVRPWCKTADYWDVYFDTLQAIKEGLDSEGIEIPFPQMDVHLNKAD
>Mature_285_residues
ADQGAVTDLATTGVANLERWLTDNSELFITYGVNIISALVILFIGNIIVKKVAGSVSKVLEKKKMDKAVVDFAHTLVRYL
LFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSLSNFAAGVLIVAFRPFKSGDYVEIGGVAGSVESIQIFQTILT
TPDNKMIVVPNGGVIGSPITNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLEADPRVLKTPAPTVGVVALADSSVNFV
VRPWCKTADYWDVYFDTLQAIKEGLDSEGIEIPFPQMDVHLNKAD

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=264, Percent_Identity=50.3787878787879, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1786670, Length=257, Percent_Identity=26.8482490272374, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI2367355, Length=255, Percent_Identity=25.4901960784314, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787591, Length=292, Percent_Identity=25.3424657534247, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 30512; Mature: 30381

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQGAVTDLATTGVANLERWLTDNSELFITYGVNIISALVILFIGNIIVKKVAGSVSKV
CCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKKKMDKAVVDFAHTLVRYLLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCH
SNFAAGVLIVAFRPFKSGDYVEIGGVAGSVESIQIFQTILTTPDNKMIVVPNGGVIGSPI
HHHHHHEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEECCCC
TNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLEADPRVLKTPAPTVGVVALADSSVNF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCCE
VVRPWCKTADYWDVYFDTLQAIKEGLDSEGIEIPFPQMDVHLNKAD
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
ADQGAVTDLATTGVANLERWLTDNSELFITYGVNIISALVILFIGNIIVKKVAGSVSKV
CCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKKKMDKAVVDFAHTLVRYLLFIIVLIAALGKVGVQTASVVAVIGAAGLAVGLALQGSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCH
SNFAAGVLIVAFRPFKSGDYVEIGGVAGSVESIQIFQTILTTPDNKMIVVPNGGVIGSPI
HHHHHHEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCEECCCC
TNYSRHATRRVDHVIGVSYSADLKKTKEVITKVLEADPRVLKTPAPTVGVVALADSSVNF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCCE
VVRPWCKTADYWDVYFDTLQAIKEGLDSEGIEIPFPQMDVHLNKAD
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]