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The map label for this gene is pcm

Identifier: 209695926

GI number: 209695926

Start: 2688196

End: 2688822

Strand: Reverse

Name: pcm

Synonym: VSAL_I2508

Alternate gene names: 209695926

Gene position: 2688822-2688196 (Counterclockwise)

Preceding gene: 209695927

Following gene: 209695925

Centisome position: 80.86

GC content: 42.58

Gene sequence:

>627_bases
ATGATGCATTCTCGCTCTGAGTTACTTGATCAATTCTTACGTCAGCAAGGTATCCTCAATGAGGATATTCTTTTGGCTAT
CCGACAATTGCCACGTGAGCGATTTGTTCCTGAAGCATTAGCACATCAAGCCTATCAAAATAATGCGCTACCAATCGGTG
AGGGTCAGACGATCTCTCAACCGTATATTGTTGCAAAAATGACGGAACTATTGGAGCTTCAATATGACTCCAACTTGTTA
GAGATTGGAACAGGCTCAGGTTATCAAACCGCAGTATTAGCCAAATTAGTAGGGCACGTGCACTCTGTTGAGCGTATTAA
ATCATTACAATGGAACGCGAAGCGCTTATTAAAGCTGTTAGATCTTTATAACATTTCAACTAAGCATGCTGATGGTTGGA
ATGGTTGGCCAAGTAAAAGCCCGTTTGATGCCATCATCGTTACCGCTGCCGCAGAATCCATTCCTAATGATCTTCTTTAT
CAATTAAAAGAGGGAGGACGGTTAGTCATTCCCATTGGTACAGAGTCACAACAATTGCTCCGAATTACCCGTCATGGAGA
TGAATTCCACTCTGAAGTGATCGAAGAGGTGCGGTTTGTGCCTTTAGTTTCAGGGGACTTAGCATAA

Upstream 100 bases:

>100_bases
ATAACTGTGTGTCTATTACCCCTTTACAAGTCGATTTAACAGCACATGATTCATTAACAATGATGAAAGAGTGGCTATCA
TTGCCGGAGAGCACTCATTA

Downstream 100 bases:

>100_bases
GTGAATATGGATAAAATAAAACAATACTTCTCTTTCTCTTACTTGGGTGTGATAGCTCTTAGTTTATTGCTTGTTGGCTG
TTCATTGGGGCCACATCGTC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MMHSRSELLDQFLRQQGILNEDILLAIRQLPRERFVPEALAHQAYQNNALPIGEGQTISQPYIVAKMTELLELQYDSNLL
EIGTGSGYQTAVLAKLVGHVHSVERIKSLQWNAKRLLKLLDLYNISTKHADGWNGWPSKSPFDAIIVTAAAESIPNDLLY
QLKEGGRLVIPIGTESQQLLRITRHGDEFHSEVIEEVRFVPLVSGDLA

Sequences:

>Translated_208_residues
MMHSRSELLDQFLRQQGILNEDILLAIRQLPRERFVPEALAHQAYQNNALPIGEGQTISQPYIVAKMTELLELQYDSNLL
EIGTGSGYQTAVLAKLVGHVHSVERIKSLQWNAKRLLKLLDLYNISTKHADGWNGWPSKSPFDAIIVTAAAESIPNDLLY
QLKEGGRLVIPIGTESQQLLRITRHGDEFHSEVIEEVRFVPLVSGDLA
>Mature_208_residues
MMHSRSELLDQFLRQQGILNEDILLAIRQLPRERFVPEALAHQAYQNNALPIGEGQTISQPYIVAKMTELLELQYDSNLL
EIGTGSGYQTAVLAKLVGHVHSVERIKSLQWNAKRLLKLLDLYNISTKHADGWNGWPSKSPFDAIIVTAAAESIPNDLLY
QLKEGGRLVIPIGTESQQLLRITRHGDEFHSEVIEEVRFVPLVSGDLA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=184, Percent_Identity=33.695652173913, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789100, Length=201, Percent_Identity=63.681592039801, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71983477, Length=213, Percent_Identity=29.1079812206573, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=217, Percent_Identity=27.6497695852535, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17981723, Length=189, Percent_Identity=30.1587301587302, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_ALISL (B6EKL2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263856.1
- ProteinModelPortal:   B6EKL2
- GeneID:   6987796
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2508
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   PRK00312
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23359; Mature: 23359

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHSRSELLDQFLRQQGILNEDILLAIRQLPRERFVPEALAHQAYQNNALPIGEGQTISQ
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
PYIVAKMTELLELQYDSNLLEIGTGSGYQTAVLAKLVGHVHSVERIKSLQWNAKRLLKLL
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DLYNISTKHADGWNGWPSKSPFDAIIVTAAAESIPNDLLYQLKEGGRLVIPIGTESQQLL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCCHHHHH
RITRHGDEFHSEVIEEVRFVPLVSGDLA
HHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMHSRSELLDQFLRQQGILNEDILLAIRQLPRERFVPEALAHQAYQNNALPIGEGQTISQ
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCC
PYIVAKMTELLELQYDSNLLEIGTGSGYQTAVLAKLVGHVHSVERIKSLQWNAKRLLKLL
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DLYNISTKHADGWNGWPSKSPFDAIIVTAAAESIPNDLLYQLKEGGRLVIPIGTESQQLL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEECCCHHHHH
RITRHGDEFHSEVIEEVRFVPLVSGDLA
HHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA