Click here to switch to the map view.

The map label for this gene is ansA [H]

Identifier: 209695599

GI number: 209695599

Start: 2318948

End: 2319961

Strand: Direct

Name: ansA [H]

Synonym: VSAL_I2158

Alternate gene names: 209695599

Gene position: 2318948-2319961 (Clockwise)

Preceding gene: 209695598

Following gene: 209695601

Centisome position: 69.74

GC content: 41.12

Gene sequence:

>1014_bases
ATGGAAAGAAAACACATTTATATCGCCTACACAGGCGGCACAATCGGCATGTTAAAATCTGATGATCATGGCTATGTCCC
TGCATCAGGCTTTATGCAACATCAATTAAAGCAAATGCCAGAGTTTCACCGTGCTGAAATGCCAATTTTCACCATTCACG
AATATGAGCCATTAATCGATTCATCTGACATGACTCCAGAAGACTGGCAGCGTATTGCAGATGATATTCGTGCTAACTAC
GATAAATACGACGGATTTGTAATTCTTCATGGTACGGATACCATGGCCTACACAGCATCTGCACTTTCATTTATGCTAGA
GAACCTCGGTAAACCAGTTATTGTTACCGGTTCACAGATCCCTCTTGCAGAACTTCGCTCTGATGGCCAAAGTAACTTAT
TAAATGCACTGCACATTGCCGCAAATTATCCAATTAACGAAGTGACGTTATTTTTCAATAACAAACTTATTCGCGGTAAC
CGCAGTACAAAATCTCATGCCGATGGCTTTAACGCATTCAGTTCACCAAATTTACCTCCATTATTGGAAGCTGGTATTAA
TATCCAAATCAACGGTGTTGAAATTAATAAGCAACCTGAAGGTGAATTTACGGTTAACAACATTACACCACAGCCCATTG
GTGTCGTTACCATGTATCCAGGCATCTCTGCGGAAGTGATTAAAAATACATTACGTCAACCTGTAAATGCAATGATATTA
CTGACTTTTGGTGTTGGTAATGCTCCTCAAAATCCTGAGCTTTTGGCACAATTAAAAGAGGCTTCTGAGCGCGGCGTTGT
CGTCATCAATTTAACTCAGTGCTTATCGGGAAAAGTAAACATGGGTGGCTATGCTACGGGTTGTGCTCTTGCTGAAGCTG
GCGTGATCAGCGGCTACGATATGACACCAGAAGCAGCTCTTGCTAAATTACATTACTTATTAAGCAAAAACCTGCCTTAT
GAAACCATGCGTACGATGATGCAGCAAAATCTACGTGGTGAGTTAACTTACTAA

Upstream 100 bases:

>100_bases
ATCTGTAAAAGCATGACGAGCCCGACACATGTCGGGCTTTCTTTATGCAGAATGACCAGTATAATCCCACCACCTCCCAT
AACTCTGTACCGTTGTTATT

Downstream 100 bases:

>100_bases
CTACAGATGTCTTTAAATAATAAAAAGGCTTCCCTGATCACTCATGGGAAGCCTTTTTTAATTCTAAAAACCGAAATATA
TATTATCTTTTTTCGTTAAG

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MERKHIYIAYTGGTIGMLKSDDHGYVPASGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFNAFSSPNLPPLLEAGINIQINGVEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVINLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYLLSKNLPY
ETMRTMMQQNLRGELTY

Sequences:

>Translated_337_residues
MERKHIYIAYTGGTIGMLKSDDHGYVPASGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFNAFSSPNLPPLLEAGINIQINGVEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVINLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYLLSKNLPY
ETMRTMMQQNLRGELTY
>Mature_337_residues
MERKHIYIAYTGGTIGMLKSDDHGYVPASGFMQHQLKQMPEFHRAEMPIFTIHEYEPLIDSSDMTPEDWQRIADDIRANY
DKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGN
RSTKSHADGFNAFSSPNLPPLLEAGINIQINGVEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
LTFGVGNAPQNPELLAQLKEASERGVVVINLTQCLSGKVNMGGYATGCALAEAGVISGYDMTPEAALAKLHYLLSKNLPY
ETMRTMMQQNLRGELTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=359, Percent_Identity=45.125348189415, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1788065, Length=336, Percent_Identity=74.7023809523809, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1789327, Length=286, Percent_Identity=25.5244755244755, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=361, Percent_Identity=47.0914127423823, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=47.0914127423823, Blast_Score=313, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI17558278, Length=361, Percent_Identity=47.0914127423823, Blast_Score=313, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI193207374, Length=361, Percent_Identity=47.0914127423823, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6320527, Length=179, Percent_Identity=29.0502793296089, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24639609, Length=364, Percent_Identity=43.6813186813187, Blast_Score=297, Evalue=8e-81,
Organism=Drosophila melanogaster, GI24645530, Length=361, Percent_Identity=44.5983379501385, Blast_Score=291, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37110; Mature: 37110

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKHIYIAYTGGTIGMLKSDDHGYVPASGFMQHQLKQMPEFHRAEMPIFTIHEYEPLID
CCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCC
SSDMTPEDWQRIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGNRSTKSHADGFNAFSSPNLPP
CHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQINGVEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
HHHCCCEEEEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
LTFGVGNAPQNPELLAQLKEASERGVVVINLTQCLSGKVNMGGYATGCALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEEHHHHHHHCCCCCCCC
MTPEAALAKLHYLLSKNLPYETMRTMMQQNLRGELTY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MERKHIYIAYTGGTIGMLKSDDHGYVPASGFMQHQLKQMPEFHRAEMPIFTIHEYEPLID
CCCCEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCC
SSDMTPEDWQRIADDIRANYDKYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQI
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCC
PLAELRSDGQSNLLNALHIAANYPINEVTLFFNNKLIRGNRSTKSHADGFNAFSSPNLPP
CHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCH
LLEAGINIQINGVEINKQPEGEFTVNNITPQPIGVVTMYPGISAEVIKNTLRQPVNAMIL
HHHCCCEEEEEEEEECCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEE
LTFGVGNAPQNPELLAQLKEASERGVVVINLTQCLSGKVNMGGYATGCALAEAGVISGYD
EEECCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCEEHHHHHHHCCCCCCCC
MTPEAALAKLHYLLSKNLPYETMRTMMQQNLRGELTY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]