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The map label for this gene is adhE [H]

Identifier: 209695322

GI number: 209695322

Start: 1989295

End: 1991946

Strand: Direct

Name: adhE [H]

Synonym: VSAL_I1855

Alternate gene names: 209695322

Gene position: 1989295-1991946 (Clockwise)

Preceding gene: 209695320

Following gene: 209695326

Centisome position: 59.83

GC content: 41.67

Gene sequence:

>2652_bases
ATGCCTGTAACTAATTTAGCTGAACTTGATGCTCTAGTAGCACGTGTTAAAGCCGCGCAAAAAGAATTTGCAAGCTTTTC
ACAAGAACAAGTTGATACAATATTTCGTGCTGCGTCTCTAGCTGCTTCAACTGCTCGTATCGAACTTGCAAAAATGGCAG
CAGAAGAGTCTGGCATGGGCATCATGGAAGACAAAGTAATCAAAAACCATTTTGCTTCTGAGTTCATCTACAATAAATAC
AAAGACGAACTAACGTGTGGCATTATCGATCGTAACGATGAAGGCGGCACGATCACAATCGCTGAACCTCTAGGTATTGT
TTGTGCAATCGTTCCAACGACAAACCCTACTTCAACAGCAATCTTTAAAGCACTAATCTGTCTTAAAACACGTAATGGCT
GTATCTTCTCACCGCACCCACGTGCTAAAAATGCAACTAACTACGCTGCTAAAGTCGTATTAGAAGCAGCAATTAAAGCG
GGCGCACCAAAAGACATCATTGGTTGGATTGACCAACCATCTGTTGAACTATCAAACGCACTAATGAAACATGAACACAT
CAACATGATCCTTGCTACTGGTGGTCCAGGCATGGTGAAAGCGGCTTATTCATCAGGTAAACCAGCTATCGGTGTTGGTG
CGGGTAATACGCCTGTCGTTATCGATGAAACTGCTGATATCAAACGTGCTGTTTCTTCTATCTTAATGTCTAAAACATTC
GATAACGGTGTTATCTGTGCTTCTGAGCAAGCTGCAATTGTTGTTGAGTCAATTTACGACGAAGTTAAAGCTCGTTTCGC
TAAATACGGCGCTGTTATTTTAAGCAAAGAAGACGCAAATAAAGTACGTAAAGTACTACTTATTGATGGCGCACTTAACG
CTAACATCGTTGGCCAACCAGCATACAAAATCGCTGAGCTAGCAAATGTTAAAGTACCTACTTCTACTAAAATTCTTATC
GGTGAAGGCTTAGAAGCTTCATGGGATGACGAATTTGCTCATGAAAAGCTATCTCCTACATTAGGTTTATTCAAAGCGAA
AGACTTTGAAGATGCTGTGCGTCAAGCAGGTATCGTATTAGACATAGGCGGTGTTGGTCATACGTCAGTACTTTACACAG
ACCAAGATAACAACGAAGAACGTATTGCGTACTTCGGTGACAAAATGAAAACCGCTCGTATTCTTGTAAACACGCCAGCA
TCTCAAGGTGGTATTGGTGACCTTTACAACTTCGAATTGGCTCCTTCATTAACACTAGGTTGTGGTTCTTGGGGTGGTAA
TGCTATCTCTGAAAACGTAGGTCCTAAGCACCTTATCAACAAGAAAATTGTAGCTAAGCGAGCAGAAAATATGTTGTGGC
ATAAACTACCAAAATCTATCTACTTCCGTCGCGGCTGTTTGCCAATCGCAATGAGCGATCTTGAAGGTAAAAAACGTGCA
CTGATCGTTACTGACCGTTTCTTATTTAACAACGGTTACATCGATGATCTACGTTCAATCTTGAAAGAGAAAGGCATGGA
AGTTGAAGTGTTCCATGAAGTAGAAGCGGATCCTACATTAGCCGTAGTTAAAGCGGGTGCAGAAGCATGTGCAAGCTACC
AACCTGATGTTATCTTAGCGGTTGGTGGTGGTTCACCAATGGATGCTGCGAAAATCATGTGGGTGATGTACGAACATCCA
GAAACTGATTTCGAAGATTTATCTATGCGCTTTATGGATATCCGTAAACGTATTTACAAGTTCCCTAAAATGGGCCAAAA
AGCTGAACTAGTATGTATTACAACTACTTCAGGTACTGGTTCTGAAGTTACACCATTCGCTGTTGTAACCGATGAAAAAA
CAGGTCAAAAATACCCATTAGCAGATTACGAGCTAACGCCAAACATGGCTGTTGTTGATGCTAACCTTGTCATGGATATG
CCTAAGTCTTTATGTGCATTCGGTGGTTACGATGCAGTAACGCACGCATTAGAAGCGTATGTTTCTGTTCTTGCAAACGA
ATACTCAGATGGTCACGCTCTGCAATCACTTAAGATGCTTAAAGAGTACTTACCAAGCTCTTACGCTAACGGTAAGAAAG
ATCCAATCGCACGTGAGAAAGTTCACAATGCAGCAACAATCGCAGGTATGTCTTTTGCGCAGTCTTTCCTAGGTGTGTGT
CACTCAATGGCGCATAAATTAGGCGCTGAATTCCATATTCCACACGGTCTTGCTAATGCGTTATTAATCTCGAATACGGT
TCGTTTTAATGCGACTAACAACCCAACTAAACAAGCGGCATTCTCTCAGTACGACCGTCCTAAAGCACGTGCTCGTTACG
CTGAAATTGCAGAGCATTTAGGTTACCGTGATGGTAATACTGAAACGAAAGTTAATGCGTTACTTAACTGGTTAGAAGAG
CTGAAAATTGCATTGGATATCCCACTGTCAATTCAAGCCGCTGGCGTTAACGAAGCTGAATTCATGGCTAAAGTAGACAA
ATTAGCTGAAGATGCATTTGATGACCAATGTACTGGTGCTAACCCACGTTACCCATTGATCAAAGAACTTAAAGAAGTAT
TAATCGCTTCTTACTACGGTACGGCTTACGCTGACGTAATGGACGCACCAGAAGTGAAAGAAGTAGTAAAAGCGAAAAAA
GCAAAGAAATAA

Upstream 100 bases:

>100_bases
ACTTAAACGATTAAGCTTAGATGCAAGAAAGGATGTAACGCAGGATCCTTACTAAATAGTTTTTAATGAATTTTATAGTT
TTATTTTTTGGAGAAACAAT

Downstream 100 bases:

>100_bases
TTCGATGCTAATAAGTTAAGGTATTTTCTCCCATATTTTGAAAGTACCTGACTAATAAAAAAACCACCTATTAGATAGAG
GTGGTTTTTTTATGCCTAAA

Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 883; Mature: 882

Protein sequence:

>883_residues
MPVTNLAELDALVARVKAAQKEFASFSQEQVDTIFRAASLAASTARIELAKMAAEESGMGIMEDKVIKNHFASEFIYNKY
KDELTCGIIDRNDEGGTITIAEPLGIVCAIVPTTNPTSTAIFKALICLKTRNGCIFSPHPRAKNATNYAAKVVLEAAIKA
GAPKDIIGWIDQPSVELSNALMKHEHINMILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVSSILMSKTF
DNGVICASEQAAIVVESIYDEVKARFAKYGAVILSKEDANKVRKVLLIDGALNANIVGQPAYKIAELANVKVPTSTKILI
GEGLEASWDDEFAHEKLSPTLGLFKAKDFEDAVRQAGIVLDIGGVGHTSVLYTDQDNNEERIAYFGDKMKTARILVNTPA
SQGGIGDLYNFELAPSLTLGCGSWGGNAISENVGPKHLINKKIVAKRAENMLWHKLPKSIYFRRGCLPIAMSDLEGKKRA
LIVTDRFLFNNGYIDDLRSILKEKGMEVEVFHEVEADPTLAVVKAGAEACASYQPDVILAVGGGSPMDAAKIMWVMYEHP
ETDFEDLSMRFMDIRKRIYKFPKMGQKAELVCITTTSGTGSEVTPFAVVTDEKTGQKYPLADYELTPNMAVVDANLVMDM
PKSLCAFGGYDAVTHALEAYVSVLANEYSDGHALQSLKMLKEYLPSSYANGKKDPIAREKVHNAATIAGMSFAQSFLGVC
HSMAHKLGAEFHIPHGLANALLISNTVRFNATNNPTKQAAFSQYDRPKARARYAEIAEHLGYRDGNTETKVNALLNWLEE
LKIALDIPLSIQAAGVNEAEFMAKVDKLAEDAFDDQCTGANPRYPLIKELKEVLIASYYGTAYADVMDAPEVKEVVKAKK
AKK

Sequences:

>Translated_883_residues
MPVTNLAELDALVARVKAAQKEFASFSQEQVDTIFRAASLAASTARIELAKMAAEESGMGIMEDKVIKNHFASEFIYNKY
KDELTCGIIDRNDEGGTITIAEPLGIVCAIVPTTNPTSTAIFKALICLKTRNGCIFSPHPRAKNATNYAAKVVLEAAIKA
GAPKDIIGWIDQPSVELSNALMKHEHINMILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVSSILMSKTF
DNGVICASEQAAIVVESIYDEVKARFAKYGAVILSKEDANKVRKVLLIDGALNANIVGQPAYKIAELANVKVPTSTKILI
GEGLEASWDDEFAHEKLSPTLGLFKAKDFEDAVRQAGIVLDIGGVGHTSVLYTDQDNNEERIAYFGDKMKTARILVNTPA
SQGGIGDLYNFELAPSLTLGCGSWGGNAISENVGPKHLINKKIVAKRAENMLWHKLPKSIYFRRGCLPIAMSDLEGKKRA
LIVTDRFLFNNGYIDDLRSILKEKGMEVEVFHEVEADPTLAVVKAGAEACASYQPDVILAVGGGSPMDAAKIMWVMYEHP
ETDFEDLSMRFMDIRKRIYKFPKMGQKAELVCITTTSGTGSEVTPFAVVTDEKTGQKYPLADYELTPNMAVVDANLVMDM
PKSLCAFGGYDAVTHALEAYVSVLANEYSDGHALQSLKMLKEYLPSSYANGKKDPIAREKVHNAATIAGMSFAQSFLGVC
HSMAHKLGAEFHIPHGLANALLISNTVRFNATNNPTKQAAFSQYDRPKARARYAEIAEHLGYRDGNTETKVNALLNWLEE
LKIALDIPLSIQAAGVNEAEFMAKVDKLAEDAFDDQCTGANPRYPLIKELKEVLIASYYGTAYADVMDAPEVKEVVKAKK
AKK
>Mature_882_residues
PVTNLAELDALVARVKAAQKEFASFSQEQVDTIFRAASLAASTARIELAKMAAEESGMGIMEDKVIKNHFASEFIYNKYK
DELTCGIIDRNDEGGTITIAEPLGIVCAIVPTTNPTSTAIFKALICLKTRNGCIFSPHPRAKNATNYAAKVVLEAAIKAG
APKDIIGWIDQPSVELSNALMKHEHINMILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVSSILMSKTFD
NGVICASEQAAIVVESIYDEVKARFAKYGAVILSKEDANKVRKVLLIDGALNANIVGQPAYKIAELANVKVPTSTKILIG
EGLEASWDDEFAHEKLSPTLGLFKAKDFEDAVRQAGIVLDIGGVGHTSVLYTDQDNNEERIAYFGDKMKTARILVNTPAS
QGGIGDLYNFELAPSLTLGCGSWGGNAISENVGPKHLINKKIVAKRAENMLWHKLPKSIYFRRGCLPIAMSDLEGKKRAL
IVTDRFLFNNGYIDDLRSILKEKGMEVEVFHEVEADPTLAVVKAGAEACASYQPDVILAVGGGSPMDAAKIMWVMYEHPE
TDFEDLSMRFMDIRKRIYKFPKMGQKAELVCITTTSGTGSEVTPFAVVTDEKTGQKYPLADYELTPNMAVVDANLVMDMP
KSLCAFGGYDAVTHALEAYVSVLANEYSDGHALQSLKMLKEYLPSSYANGKKDPIAREKVHNAATIAGMSFAQSFLGVCH
SMAHKLGAEFHIPHGLANALLISNTVRFNATNNPTKQAAFSQYDRPKARARYAEIAEHLGYRDGNTETKVNALLNWLEEL
KIALDIPLSIQAAGVNEAEFMAKVDKLAEDAFDDQCTGANPRYPLIKELKEVLIASYYGTAYADVMDAPEVKEVVKAKKA
KK

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=384, Percent_Identity=25, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1787493, Length=869, Percent_Identity=74.5684695051784, Blast_Score=1340, Evalue=0.0,
Organism=Escherichia coli, GI48994951, Length=385, Percent_Identity=33.7662337662338, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789163, Length=413, Percent_Identity=31.7191283292978, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI1788797, Length=370, Percent_Identity=33.7837837837838, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI87082107, Length=376, Percent_Identity=30.3191489361702, Blast_Score=179, Evalue=1e-45,
Organism=Escherichia coli, GI1789386, Length=389, Percent_Identity=26.9922879177378, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=302, Percent_Identity=26.158940397351, Blast_Score=104, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321181, Length=391, Percent_Identity=31.4578005115089, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24657991, Length=320, Percent_Identity=25, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 95863; Mature: 95732

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTNLAELDALVARVKAAQKEFASFSQEQVDTIFRAASLAASTARIELAKMAAEESGMG
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IMEDKVIKNHFASEFIYNKYKDELTCGIIDRNDEGGTITIAEPLGIVCAIVPTTNPTSTA
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCHHH
IFKALICLKTRNGCIFSPHPRAKNATNYAAKVVLEAAIKAGAPKDIIGWIDQPSVELSNA
HHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMKHEHINMILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVSSILMSKTF
HHHHCCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVICASEQAAIVVESIYDEVKARFAKYGAVILSKEDANKVRKVLLIDGALNANIVGQP
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHEEEEEEECCCCCCEECCC
AYKIAELANVKVPTSTKILIGEGLEASWDDEFAHEKLSPTLGLFKAKDFEDAVRQAGIVL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEE
DIGGVGHTSVLYTDQDNNEERIAYFGDKMKTARILVNTPASQGGIGDLYNFELAPSLTLG
EECCCCCCEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNAISENVGPKHLINKKIVAKRAENMLWHKLPKSIYFRRGCLPIAMSDLEGKKRA
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEECCCCCCCEE
LIVTDRFLFNNGYIDDLRSILKEKGMEVEVFHEVEADPTLAVVKAGAEACASYQPDVILA
EEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHCHHHHHCCCCCEEEE
VGGGSPMDAAKIMWVMYEHPETDFEDLSMRFMDIRKRIYKFPKMGQKAELVCITTTSGTG
ECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
SEVTPFAVVTDEKTGQKYPLADYELTPNMAVVDANLVMDMPKSLCAFGGYDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEEEHHHHHHCHHHHHHCCCHHHHHHHHHHH
VSVLANEYSDGHALQSLKMLKEYLPSSYANGKKDPIAREKVHNAATIAGMSFAQSFLGVC
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HSMAHKLGAEFHIPHGLANALLISNTVRFNATNNPTKQAAFSQYDRPKARARYAEIAEHL
HHHHHHCCCCEECCCHHHHHHHEECEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
GYRDGNTETKVNALLNWLEELKIALDIPLSIQAAGVNEAEFMAKVDKLAEDAFDDQCTGA
CCCCCCCHHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLIKELKEVLIASYYGTAYADVMDAPEVKEVVKAKKAKK
CCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVTNLAELDALVARVKAAQKEFASFSQEQVDTIFRAASLAASTARIELAKMAAEESGMG
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IMEDKVIKNHFASEFIYNKYKDELTCGIIDRNDEGGTITIAEPLGIVCAIVPTTNPTSTA
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCHHH
IFKALICLKTRNGCIFSPHPRAKNATNYAAKVVLEAAIKAGAPKDIIGWIDQPSVELSNA
HHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHH
LMKHEHINMILATGGPGMVKAAYSSGKPAIGVGAGNTPVVIDETADIKRAVSSILMSKTF
HHHHCCCEEEEEECCCCEEEEECCCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHHCC
DNGVICASEQAAIVVESIYDEVKARFAKYGAVILSKEDANKVRKVLLIDGALNANIVGQP
CCCEEEECCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHEEEEEEECCCCCCEECCC
AYKIAELANVKVPTSTKILIGEGLEASWDDEFAHEKLSPTLGLFKAKDFEDAVRQAGIVL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHCCCCHHHHHHHCCEEE
DIGGVGHTSVLYTDQDNNEERIAYFGDKMKTARILVNTPASQGGIGDLYNFELAPSLTLG
EECCCCCCEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEE
CGSWGGNAISENVGPKHLINKKIVAKRAENMLWHKLPKSIYFRRGCLPIAMSDLEGKKRA
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEECCCCCCCEE
LIVTDRFLFNNGYIDDLRSILKEKGMEVEVFHEVEADPTLAVVKAGAEACASYQPDVILA
EEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEEHHCHHHHHCCCCCEEEE
VGGGSPMDAAKIMWVMYEHPETDFEDLSMRFMDIRKRIYKFPKMGQKAELVCITTTSGTG
ECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
SEVTPFAVVTDEKTGQKYPLADYELTPNMAVVDANLVMDMPKSLCAFGGYDAVTHALEAY
CCCCCEEEEECCCCCCCCCCCCCEECCCEEEEEHHHHHHCHHHHHHCCCHHHHHHHHHHH
VSVLANEYSDGHALQSLKMLKEYLPSSYANGKKDPIAREKVHNAATIAGMSFAQSFLGVC
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HSMAHKLGAEFHIPHGLANALLISNTVRFNATNNPTKQAAFSQYDRPKARARYAEIAEHL
HHHHHHCCCCEECCCHHHHHHHEECEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHC
GYRDGNTETKVNALLNWLEELKIALDIPLSIQAAGVNEAEFMAKVDKLAEDAFDDQCTGA
CCCCCCCHHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
NPRYPLIKELKEVLIASYYGTAYADVMDAPEVKEVVKAKKAKK
CCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]