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The map label for this gene is ybiK [C]

Identifier: 209695155

GI number: 209695155

Start: 1756764

End: 1757726

Strand: Direct

Name: ybiK [C]

Synonym: VSAL_I1658

Alternate gene names: 209695155

Gene position: 1756764-1757726 (Clockwise)

Preceding gene: 209695154

Following gene: 209695156

Centisome position: 52.83

GC content: 42.78

Gene sequence:

>963_bases
ATGAATGAATCTAAATGGGGAATAATTGCTACTTGGCGTATGGCATTTGATGGGATCAGTGATGGTGCTGCCCGTCTGAA
AGAAAGCAAAACATCACAAGAAGCGGTGATTCAAGCGGTAAAAATAGTGGAAGATTATCCTTTTTATAAAAGTGTGGGTT
ATGGTGGTTTACCTAATGAAAACGGTGTTGTTGAATTAGACGCCGCTTTTATGAATGGGACAACAATGGCTATTGGTGCT
GTTGCTAGTATACAGAACGTAGCGAATCCAATACTCGTTGCTGAAGCATTGAGTAATGAACGATTCAACAGCTTTATGGT
TGGCTCAGGTGCCGAAAGTTGGGCGTTATCTCATGGCTTTGAAGAAAAAAACATGCTTACAGAGCGCGCAAAACAGCATT
ACGCTAAACGCTGTCAAGAAACCTTAGACCGAGGATTATCCCCTTACACTGGACATGATACGGTTGGTGTCGCTGCGGTT
GATCTTAATGGTGAAATATCAGTAGCAACCTCTACCAGTGGCTTATTTATGAAACGTGCTGGCCGTGTTGGTGATTCTCC
AATTTCAGGCTCTGGATTCTATGCAGATAGTGATATTGGCGCAGCTACCGCGACAGGGCTAGGCGAAGACTTAATGAAAG
GTTGCGTCAGCTATGAAATAGTTCGACGTATGGGCAGTGGAATGACACCACAAGAAGCGGCTGAAAGTACCGTTAATGAT
TTAGAAAAAAAACTAAAAGAACGTTATGGCCGCTCTGGTGACCTTTCTGTTGTTTGTATTAACAAGGAAGGTGAATTTGG
AGCTGCCACAAACATTGATGGCTTCTCGTTCTCTGTAGCGACTTATAAGCAACAACCAACGATATATACGTGTGTTCGTA
ATGGTGAGCACACAGAACACAGCATTGCTAGCCAAGAATGGTTAGATGCTTATCAAGCACGAATTACTGCACCTATTGTA
TAA

Upstream 100 bases:

>100_bases
CATTTTTACGAATTATTGATAAACAATACCGTTCTCAAGAAACCCCTATTGTTTCGGAAGAACCGACACCTTCTGAAGCT
ACTTTAGAGAGAGCATAATA

Downstream 100 bases:

>100_bases
TTTTGGAGTTTAAAATGAAAAAGATCCTACTTATTTGTGATATGGGTATGTCTACTAGCTTAGTCGTTAAACGCATGCTT
GAGCACGCAAAGAAAGAAAA

Product: glycosylasparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNESKWGIIATWRMAFDGISDGAARLKESKTSQEAVIQAVKIVEDYPFYKSVGYGGLPNENGVVELDAAFMNGTTMAIGA
VASIQNVANPILVAEALSNERFNSFMVGSGAESWALSHGFEEKNMLTERAKQHYAKRCQETLDRGLSPYTGHDTVGVAAV
DLNGEISVATSTSGLFMKRAGRVGDSPISGSGFYADSDIGAATATGLGEDLMKGCVSYEIVRRMGSGMTPQEAAESTVND
LEKKLKERYGRSGDLSVVCINKEGEFGAATNIDGFSFSVATYKQQPTIYTCVRNGEHTEHSIASQEWLDAYQARITAPIV

Sequences:

>Translated_320_residues
MNESKWGIIATWRMAFDGISDGAARLKESKTSQEAVIQAVKIVEDYPFYKSVGYGGLPNENGVVELDAAFMNGTTMAIGA
VASIQNVANPILVAEALSNERFNSFMVGSGAESWALSHGFEEKNMLTERAKQHYAKRCQETLDRGLSPYTGHDTVGVAAV
DLNGEISVATSTSGLFMKRAGRVGDSPISGSGFYADSDIGAATATGLGEDLMKGCVSYEIVRRMGSGMTPQEAAESTVND
LEKKLKERYGRSGDLSVVCINKEGEFGAATNIDGFSFSVATYKQQPTIYTCVRNGEHTEHSIASQEWLDAYQARITAPIV
>Mature_320_residues
MNESKWGIIATWRMAFDGISDGAARLKESKTSQEAVIQAVKIVEDYPFYKSVGYGGLPNENGVVELDAAFMNGTTMAIGA
VASIQNVANPILVAEALSNERFNSFMVGSGAESWALSHGFEEKNMLTERAKQHYAKRCQETLDRGLSPYTGHDTVGVAAV
DLNGEISVATSTSGLFMKRAGRVGDSPISGSGFYADSDIGAATATGLGEDLMKGCVSYEIVRRMGSGMTPQEAAESTVND
LEKKLKERYGRSGDLSVVCINKEGEFGAATNIDGFSFSVATYKQQPTIYTCVRNGEHTEHSIASQEWLDAYQARITAPIV

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=317, Percent_Identity=33.4384858044164, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI145275202, Length=244, Percent_Identity=34.0163934426229, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI145275200, Length=244, Percent_Identity=34.0163934426229, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI285002253, Length=317, Percent_Identity=31.8611987381703, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI98991769, Length=258, Percent_Identity=27.1317829457364, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1787050, Length=267, Percent_Identity=32.2097378277154, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17569239, Length=270, Percent_Identity=31.1111111111111, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564649, Length=158, Percent_Identity=31.0126582278481, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24652135, Length=315, Percent_Identity=32.3809523809524, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221330102, Length=315, Percent_Identity=32.3809523809524, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24655553, Length=321, Percent_Identity=30.2180685358255, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24657358, Length=310, Percent_Identity=28.3870967741935, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI18921183, Length=290, Percent_Identity=30, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357893, Length=237, Percent_Identity=29.535864978903, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 34378; Mature: 34378

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESKWGIIATWRMAFDGISDGAARLKESKTSQEAVIQAVKIVEDYPFYKSVGYGGLPNE
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
NGVVELDAAFMNGTTMAIGAVASIQNVANPILVAEALSNERFNSFMVGSGAESWALSHGF
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCC
EEKNMLTERAKQHYAKRCQETLDRGLSPYTGHDTVGVAAVDLNGEISVATSTSGLFMKRA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHC
GRVGDSPISGSGFYADSDIGAATATGLGEDLMKGCVSYEIVRRMGSGMTPQEAAESTVND
CCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEKKLKERYGRSGDLSVVCINKEGEFGAATNIDGFSFSVATYKQQPTIYTCVRNGEHTEH
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHH
SIASQEWLDAYQARITAPIV
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNESKWGIIATWRMAFDGISDGAARLKESKTSQEAVIQAVKIVEDYPFYKSVGYGGLPNE
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
NGVVELDAAFMNGTTMAIGAVASIQNVANPILVAEALSNERFNSFMVGSGAESWALSHGF
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCC
EEKNMLTERAKQHYAKRCQETLDRGLSPYTGHDTVGVAAVDLNGEISVATSTSGLFMKRA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHC
GRVGDSPISGSGFYADSDIGAATATGLGEDLMKGCVSYEIVRRMGSGMTPQEAAESTVND
CCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LEKKLKERYGRSGDLSVVCINKEGEFGAATNIDGFSFSVATYKQQPTIYTCVRNGEHTEH
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHH
SIASQEWLDAYQARITAPIV
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]