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The map label for this gene is malI [H]

Identifier: 209695119

GI number: 209695119

Start: 1725340

End: 1726362

Strand: Direct

Name: malI [H]

Synonym: VSAL_I1618

Alternate gene names: 209695119

Gene position: 1725340-1726362 (Clockwise)

Preceding gene: 209695118

Following gene: 209695121

Centisome position: 51.89

GC content: 38.61

Gene sequence:

>1023_bases
ATGACTAACAAAAAAGCAAATATAAAGGATGTTGCAAAACGAGCGGGAGTCTCAGTAACAACCGTATCAATGGCACTGAG
TGATAAAGGACGGATCTCACCTGAAACCATTCAAAAAGTAAATCAAGCGGTACAAGAACTCGGCTACATTAAAAATCGCA
CCGCTTCTAATTTAAGTTCAAAACAATCATTATTGATTGGCTTAATTTTAAGAGACATCAGCGACCCCTATTACGCTGAA
ATAGCAGCAGGATTAAGTGAAGTCTTAGAAGATAAGGGATACATGCTTTTTCTTACCCAATCAGGCACAAATCAAGATAA
GTTTGATCAATGTATTGAAACCATGGTGGCTCAAAATGTTGGTGGGATTGTATTTTGCCCTATCCGTGATATTGGCGTTC
CGAATATTCAAAAAATACAAGATTTAAACTTACCAATAGTCTGTGTTGCTCGAGCCAAAGTAGGGCAGCAGATTGACTTT
GTTGGGCCCGATAATATGCAAGCTGCGAAAATTGCTACAGAGTATTTGATTAAACAAGGGCACCGTCAAATAGCTTACGT
TGGCGGACAAAGTGATTCATTAAGTAGAGCCGAACGTTTAGGTGGTTATTGCAGCACACTAATGCAATTTGGCTTACCTT
TTAAACCTGAATGGGTTGTTGAATGTGATAAAAGTCAAACTGCCGCCGCAGAAGCAGTAAATCAACTAATTAATAAAAAT
CCAAAAATAACAGCAATACTATGTCATTATTCATCAACCGCATTTGGTGCTGTTTATGGTGTTAATCGCTCTAATCGCAC
AGTGGGTAAAGATAATTACATTGGACAACAAGTCGCTATTATTGGATTTGATGATGTCCCTGAAGCCGAGTTAATTCAAC
CTTCATTAACCTTTGTCTCCTCCCCTATTCGTGACATTGGTCGCCAAGCTGGCCACCGATTAATTCACCAAATAAACAAT
AAAGACAGTGCACCTCAAAGTCTTATTTTAAATCCAGAATTAATTGCTCGTGACTCAGCTTAG

Upstream 100 bases:

>100_bases
ACGATCTTTTTCCATTAGATTGATAATTACGCTAGAATGGCGTCAATAAATGTGTTTAATTAGGTAAAACGTTTTATCTA
ATCGTGGTAAAAGAAGTGCT

Downstream 100 bases:

>100_bases
TTAAACCATACTCATAAGTAAAAATCACATATAAAAAAGGTATTTGGGTTAGTCATATTTTATTACAGCCCAAAGACTTA
CTACTAAGTTCGTAATCTAT

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTNKKANIKDVAKRAGVSVTTVSMALSDKGRISPETIQKVNQAVQELGYIKNRTASNLSSKQSLLIGLILRDISDPYYAE
IAAGLSEVLEDKGYMLFLTQSGTNQDKFDQCIETMVAQNVGGIVFCPIRDIGVPNIQKIQDLNLPIVCVARAKVGQQIDF
VGPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKPEWVVECDKSQTAAAEAVNQLINKN
PKITAILCHYSSTAFGAVYGVNRSNRTVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLIHQINN
KDSAPQSLILNPELIARDSA

Sequences:

>Translated_340_residues
MTNKKANIKDVAKRAGVSVTTVSMALSDKGRISPETIQKVNQAVQELGYIKNRTASNLSSKQSLLIGLILRDISDPYYAE
IAAGLSEVLEDKGYMLFLTQSGTNQDKFDQCIETMVAQNVGGIVFCPIRDIGVPNIQKIQDLNLPIVCVARAKVGQQIDF
VGPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKPEWVVECDKSQTAAAEAVNQLINKN
PKITAILCHYSSTAFGAVYGVNRSNRTVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLIHQINN
KDSAPQSLILNPELIARDSA
>Mature_339_residues
TNKKANIKDVAKRAGVSVTTVSMALSDKGRISPETIQKVNQAVQELGYIKNRTASNLSSKQSLLIGLILRDISDPYYAEI
AAGLSEVLEDKGYMLFLTQSGTNQDKFDQCIETMVAQNVGGIVFCPIRDIGVPNIQKIQDLNLPIVCVARAKVGQQIDFV
GPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKPEWVVECDKSQTAAAEAVNQLINKNP
KITAILCHYSSTAFGAVYGVNRSNRTVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLIHQINNK
DSAPQSLILNPELIARDSA

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=338, Percent_Identity=43.1952662721894, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1790369, Length=336, Percent_Identity=29.1666666666667, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=31.5315315315315, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787948, Length=337, Percent_Identity=29.080118694362, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790194, Length=341, Percent_Identity=27.8592375366569, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789202, Length=338, Percent_Identity=29.2899408284024, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994940, Length=332, Percent_Identity=28.3132530120482, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=27.4626865671642, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=25.3822629969419, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=25.4491017964072, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786268, Length=307, Percent_Identity=25.0814332247557, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790689, Length=334, Percent_Identity=23.0538922155689, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37041; Mature: 36910

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKKANIKDVAKRAGVSVTTVSMALSDKGRISPETIQKVNQAVQELGYIKNRTASNLSS
CCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHH
KQSLLIGLILRDISDPYYAEIAAGLSEVLEDKGYMLFLTQSGTNQDKFDQCIETMVAQNV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
GGIVFCPIRDIGVPNIQKIQDLNLPIVCVARAKVGQQIDFVGPDNMQAAKIATEYLIKQG
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEHHCCCEEECCCCCCCHHHHHHHHHHHHCC
HRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKPEWVVECDKSQTAAAEAVNQLINKN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC
PKITAILCHYSSTAFGAVYGVNRSNRTVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVS
CCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHH
SPIRDIGRQAGHRLIHQINNKDSAPQSLILNPELIARDSA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCC
>Mature Secondary Structure 
TNKKANIKDVAKRAGVSVTTVSMALSDKGRISPETIQKVNQAVQELGYIKNRTASNLSS
CCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHH
KQSLLIGLILRDISDPYYAEIAAGLSEVLEDKGYMLFLTQSGTNQDKFDQCIETMVAQNV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
GGIVFCPIRDIGVPNIQKIQDLNLPIVCVARAKVGQQIDFVGPDNMQAAKIATEYLIKQG
CCEEEECCCCCCCCCHHHHHHCCCCEEEEEEHHCCCEEECCCCCCCHHHHHHHHHHHHCC
HRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKPEWVVECDKSQTAAAEAVNQLINKN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCC
PKITAILCHYSSTAFGAVYGVNRSNRTVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVS
CCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHH
SPIRDIGRQAGHRLIHQINNKDSAPQSLILNPELIARDSA
HHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]