Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 209695084

GI number: 209695084

Start: 1691299

End: 1692156

Strand: Direct

Name: potB [H]

Synonym: VSAL_I1581

Alternate gene names: 209695084

Gene position: 1691299-1692156 (Clockwise)

Preceding gene: 209695083

Following gene: 209695085

Centisome position: 50.86

GC content: 36.6

Gene sequence:

>858_bases
ATGAACAAGAAGTTTAATCTCCAAAATATCATCATCACCGTTATTGTAACGTGGTTACTGTTGTTCGTGTTCATTCCTAA
TGTAATGATCATAGGCACAAGTTTCCTTACTCGTGATGAAGCAAATTTAATCGAAATGACGTTTACACTAGATAACTATC
TTCGTCTTTTTGATCCTTTGTACGCAAAAGTACTGTGGCATTCATTTTATATGGCCATTGTTGCAACACTACTGTGTTTA
GTTGTGGGTTACCCATTTGCTTACATTGTTGCAAAAATGCCAGAAAAATGGCGCCCATTTATGCTGTTTTTAATCATTGT
TCCTTTTTGGACTAACTCACTGATCCGTACTTACGGACTTAAAATTGTGTTAGGAACTCAAGGTATTTTGAATAAAGGGT
TACTTGCGATGGATATCATTGATAAACCATTACGCATTATGTATTCAGAAACAGCGGTAATGATAGGTTTAGTCTATATC
CTATTACCATTTATGATTTTGCCTTTGTACTCTGCAATTGAAAAGCTAGATAATACCTATTTAGAAGCAGCAAAAGATCT
TGGCGCAAATAAGCTACAAACACTATTAAAAATAGTTCTGCCATTAACCATGCCAGGGATCATTGGTGGTTGCTTATTAG
TTCTACTCCCCGCTCTTGGTATGTTCTATATTTCTGATTTATTAGGCGGCGCGAAAAACCTATTAATCGGTAATGTTATT
AAAAGCCAAGTATTAAACGCTCGAGATTGGCCATTTGGTGCAGCGACCAGTATTGCCCTCACCTTGGCAATGGCATTAAT
GCTGTATGCTTATTATCGAGCTGGCAAGTTATTAAATAAAAAGGCGGATCTAGACTGA

Upstream 100 bases:

>100_bases
ATCAGTGAATTCTTTAATGAAGATGACCCAGATGTTGATCACTCTTTAGGTCAAAAAGTAGCAATTACTTGGGTTGAAAG
TTGGGAAGTGGTGCTCGCAG

Downstream 100 bases:

>100_bases
TGGGACGCGCAATAAAAATCAGCTTTATGAGTTTGGTGTATATATTCCTATACCTACCAATCATCGTTTTGATTGTAAAC
TCATTTAACGCAAGTAAATT

Product: spermidine/putrescine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNKKFNLQNIIITVIVTWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTLDNYLRLFDPLYAKVLWHSFYMAIVATLLCL
VVGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKGLLAMDIIDKPLRIMYSETAVMIGLVYI
LLPFMILPLYSAIEKLDNTYLEAAKDLGANKLQTLLKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD

Sequences:

>Translated_285_residues
MNKKFNLQNIIITVIVTWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTLDNYLRLFDPLYAKVLWHSFYMAIVATLLCL
VVGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKGLLAMDIIDKPLRIMYSETAVMIGLVYI
LLPFMILPLYSAIEKLDNTYLEAAKDLGANKLQTLLKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD
>Mature_285_residues
MNKKFNLQNIIITVIVTWLLLFVFIPNVMIIGTSFLTRDEANLIEMTFTLDNYLRLFDPLYAKVLWHSFYMAIVATLLCL
VVGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNKGLLAMDIIDKPLRIMYSETAVMIGLVYI
LLPFMILPLYSAIEKLDNTYLEAAKDLGANKLQTLLKIVLPLTMPGIIGGCLLVLLPALGMFYISDLLGGAKNLLIGNVI
KSQVLNARDWPFGAATSIALTLAMALMLYAYYRAGKLLNKKADLD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=275, Percent_Identity=69.0909090909091, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787080, Length=234, Percent_Identity=40.1709401709402, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32043; Mature: 32043

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]