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The map label for this gene is ttcA

Identifier: 209695076

GI number: 209695076

Start: 1684330

End: 1685253

Strand: Direct

Name: ttcA

Synonym: VSAL_I1573

Alternate gene names: 209695076

Gene position: 1684330-1685253 (Clockwise)

Preceding gene: 209695075

Following gene: 209695077

Centisome position: 50.65

GC content: 39.07

Gene sequence:

>924_bases
ATGAGCGAATTAACCAAAGCGCAGCAACAAAATTTCACTAAGCTTCAGAAAAAAATCCGTCGTAATACAGGTAAAGCTAT
TGCCGATTACAACATGATTGAAGATGGCGACCGTATTATGGTTTGTCTATCTGGTGGTAAAGACAGCTTTACCATGCTTG
ATATTCTTATCGGCTTAAAGAAAAGTGCGCCAATCTCATTTGATCTAATCGCTGTAAACCTAGACCAAAAACAACCTGGT
TTCCCTACTCATATTTTACCTGAGTACCTAGATACATTAGGGGTTGAATACCGAGTTGTTGAAGAAGATACCTACTCAAT
TGTTCAAGATAAATTGATTGAAGGTAAAACGACTTGTTCTTTATGTTCACGCTTACGTCGTGGTATTTTGTATCGTACAG
CAAAAGAACTGGGGGCAACGAAAATTGCACTTGGTCACCACCGTGATGACATATTAGAAACGATGTTCTTAAACATGTTC
TACGGCGGAAAACTGAAAGGCATGCCGCCAAAACTAGTGTCAGATAATGGCGAACACGTCGTGATTCGCCCACTGGCTTA
TTGTCGTGAAAAAGACATCATTAAATATGCTGACCTAGCTGACTACCCTATTATTCCATGTAATCTTTGTGGCTCTCAAC
CTAACATGCAACGCCAAAACATTAAGCAAATGCTAAATACATGGGATACACAGTTCCCAGGACGTATTGAATCGATGTTT
ACAGCAATGCAAAACGTTGTACCAAGTCACCTTGCTGATTTTAATACTTTTGACTTTAAGAGCATTAATCGAGATTCAGG
CGTGATCAATGGTGGTGACATTGGTTTTGATAAAGAAGAAATGCCAGTACAGCCCGTTGATATTGATGATGCAGTTACAG
AGTTCGACCCATCATTAAAATTAGATATTGTGAACGTACAATAA

Upstream 100 bases:

>100_bases
GCAGTAAACCAATAAACTAATTCCCTCCAAACCTCGCTTATTCGTATTCTTTGGGTATAATACCCGCCTTATCCGTTCCA
TGAGTCGATTAGAGTAAGTA

Downstream 100 bases:

>100_bases
CGTTAAACCTTTGATACAAAAATAAAAAAATGGCGCTACTTTTAAATAAGTAGCGCCATTTTTATTTAAATAAACGTAAG
CGTGCGGGGAACTACACCTA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSELTKAQQQNFTKLQKKIRRNTGKAIADYNMIEDGDRIMVCLSGGKDSFTMLDILIGLKKSAPISFDLIAVNLDQKQPG
FPTHILPEYLDTLGVEYRVVEEDTYSIVQDKLIEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMF
YGGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADLADYPIIPCNLCGSQPNMQRQNIKQMLNTWDTQFPGRIESMF
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPVQPVDIDDAVTEFDPSLKLDIVNVQ

Sequences:

>Translated_307_residues
MSELTKAQQQNFTKLQKKIRRNTGKAIADYNMIEDGDRIMVCLSGGKDSFTMLDILIGLKKSAPISFDLIAVNLDQKQPG
FPTHILPEYLDTLGVEYRVVEEDTYSIVQDKLIEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMF
YGGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADLADYPIIPCNLCGSQPNMQRQNIKQMLNTWDTQFPGRIESMF
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPVQPVDIDDAVTEFDPSLKLDIVNVQ
>Mature_306_residues
SELTKAQQQNFTKLQKKIRRNTGKAIADYNMIEDGDRIMVCLSGGKDSFTMLDILIGLKKSAPISFDLIAVNLDQKQPGF
PTHILPEYLDTLGVEYRVVEEDTYSIVQDKLIEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFY
GGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADLADYPIIPCNLCGSQPNMQRQNIKQMLNTWDTQFPGRIESMFT
AMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPVQPVDIDDAVTEFDPSLKLDIVNVQ

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=308, Percent_Identity=69.4805194805195, Blast_Score=466, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI193211090, Length=224, Percent_Identity=33.0357142857143, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=26.1603375527426, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24651972, Length=221, Percent_Identity=26.6968325791855, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_ALISL (B6ELU0)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263005.1
- GeneID:   6989362
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1573
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 34620; Mature: 34489

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTKAQQQNFTKLQKKIRRNTGKAIADYNMIEDGDRIMVCLSGGKDSFTMLDILIGLK
CCHHHHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCEEEEEEECCCCCHHHHHHHHHCC
KSAPISFDLIAVNLDQKQPGFPTHILPEYLDTLGVEYRVVEEDTYSIVQDKLIEGKTTCS
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHH
LCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFYGGKLKGMPPKLVSDNGEHV
HHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
VIRPLAYCREKDIIKYADLADYPIIPCNLCGSQPNMQRQNIKQMLNTWDTQFPGRIESMF
EECCHHHHCCCCCEEHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPVQPVDIDDAVTEFDPSLK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEE
LDIVNVQ
EEEEECC
>Mature Secondary Structure 
SELTKAQQQNFTKLQKKIRRNTGKAIADYNMIEDGDRIMVCLSGGKDSFTMLDILIGLK
CHHHHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCEEEEEEECCCCCHHHHHHHHHCC
KSAPISFDLIAVNLDQKQPGFPTHILPEYLDTLGVEYRVVEEDTYSIVQDKLIEGKTTCS
CCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCHHHH
LCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFYGGKLKGMPPKLVSDNGEHV
HHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
VIRPLAYCREKDIIKYADLADYPIIPCNLCGSQPNMQRQNIKQMLNTWDTQFPGRIESMF
EECCHHHHCCCCCEEHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPVQPVDIDDAVTEFDPSLK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEE
LDIVNVQ
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA