Click here to switch to the map view.

The map label for this gene is nagK [H]

Identifier: 209694918

GI number: 209694918

Start: 1508346

End: 1509254

Strand: Direct

Name: nagK [H]

Synonym: VSAL_I1392

Alternate gene names: 209694918

Gene position: 1508346-1509254 (Clockwise)

Preceding gene: 209694917

Following gene: 209694920

Centisome position: 45.36

GC content: 40.92

Gene sequence:

>909_bases
ATGTACTACGGTTTTGATGTGGGTGGAACAAAGATTGAATTTGGTGCGTTTAATGAAAAATTAGAACGTGTCGCGACAGA
ACGAATTCCAACTCAAACTGAAGATTATTCACTTTTAGTTAATGATATCGCCAGCTTAATCGCAAAATATGATGCGGAAT
TTGGTGTTGAAGGTAAGGTCGGTTTAGGCATTCCAGGAATGGAAGATGCAGAAACGGGCGCTTTACTTACCAGTAATGTC
CCTGCGGCAAAAGGTCAATTTTTACGAAAAGATCTGGAAGCAAAAATTGGTCGCTCAGTTAAAATTGATAATGATGCGAA
CTGTTTTGCACTATCAGAAGCATGGGATGAAGAATTAAAAGATTCACCCTCAGTGCTTGGGTTGATTCTTGGGACTGGTT
TTGGTGGTGGTTTAGTCTTTGATGGCAAAGTATTCTCAGGGTATAGCCACGTTGCGGGAGAGTTAGGCCATTCTCGTTTA
CCGATTGATGCATGGTTTCATTTAGGTGAGAAAGCACCGTTACTTGAATGTGGTTGTGGTAACAAAGGTTGTATTGATAA
TTACCTTTCTGGTCGTGGTTTTGAGTTACTTTATGCTCATTATTATGGCCAAGAAAAGAAAGCGATAGATATTATCAAAG
CAAATGAAGCGGGTGATGCCGATGCGGTTGAACACGTTGATCGCTTTATGGAATTATTGGCGATTTGTTTTGCTAATCTA
TTTACTTGCTTTGACCCACATGTCGTTGCTCTAGGTGGCGGCTTATCAAACTTTGCACTGATTTATGATGAATTACCAAA
GCGTTTACCAAAGCACTTGTTGTCGGTTGCGCGTGTACCTCGCATTATTAAAGCGAAACATGGCGATTCAGGTGGTGTTC
GTGGCGCCGCGTTCTTGAATATCAAATAA

Upstream 100 bases:

>100_bases
AAATTAGGCTTAATTCTGCGTAGGTCACAGTCTGTATTTATAAAAATTAGGTAAGATAACACCATAAAAAATTAAAATAA
ATTTAGGACAAGGATACCTT

Downstream 100 bases:

>100_bases
CGTAATGGTTAGATGATGAGATAGATAGTTACAGGTAATAAGAAAATGCACATAAAAAAAGAGCCTCATGTTAGTGATAA
CAGAGGCTCTTTTTTGTGTT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTEDYSLLVNDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVLGLILGTGFGGGLVFDGKVFSGYSHVAGELGHSRL
PIDAWFHLGEKAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGQEKKAIDIIKANEAGDADAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFALIYDELPKRLPKHLLSVARVPRIIKAKHGDSGGVRGAAFLNIK

Sequences:

>Translated_302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTEDYSLLVNDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVLGLILGTGFGGGLVFDGKVFSGYSHVAGELGHSRL
PIDAWFHLGEKAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGQEKKAIDIIKANEAGDADAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFALIYDELPKRLPKHLLSVARVPRIIKAKHGDSGGVRGAAFLNIK
>Mature_302_residues
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTEDYSLLVNDIASLIAKYDAEFGVEGKVGLGIPGMEDAETGALLTSNV
PAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELKDSPSVLGLILGTGFGGGLVFDGKVFSGYSHVAGELGHSRL
PIDAWFHLGEKAPLLECGCGNKGCIDNYLSGRGFELLYAHYYGQEKKAIDIIKANEAGDADAVEHVDRFMELLAICFANL
FTCFDPHVVALGGGLSNFALIYDELPKRLPKHLLSVARVPRIIKAKHGDSGGVRGAAFLNIK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=54.3046357615894, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87081733, Length=304, Percent_Identity=37.171052631579, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1790522, Length=311, Percent_Identity=26.6881028938907, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786891, Length=236, Percent_Identity=24.5762711864407, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 32609; Mature: 32609

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTEDYSLLVNDIASLIAKYDAEFGVEGKV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGIPGMEDAETGALLTSNVPAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHC
DSPSVLGLILGTGFGGGLVFDGKVFSGYSHVAGELGHSRLPIDAWFHLGEKAPLLECGCG
CCCHHEEHEEECCCCCCEEECCCEECCHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCC
NKGCIDNYLSGRGFELLYAHYYGQEKKAIDIIKANEAGDADAVEHVDRFMELLAICFANL
CCCCHHHHHCCCCCEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FTCFDPHVVALGGGLSNFALIYDELPKRLPKHLLSVARVPRIIKAKHGDSGGVRGAAFLN
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEE
IK
CC
>Mature Secondary Structure
MYYGFDVGGTKIEFGAFNEKLERVATERIPTQTEDYSLLVNDIASLIAKYDAEFGVEGKV
CEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GLGIPGMEDAETGALLTSNVPAAKGQFLRKDLEAKIGRSVKIDNDANCFALSEAWDEELK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHC
DSPSVLGLILGTGFGGGLVFDGKVFSGYSHVAGELGHSRLPIDAWFHLGEKAPLLECGCG
CCCHHEEHEEECCCCCCEEECCCEECCHHHHHHHCCCCCCCHHHHHHCCCCCCEEECCCC
NKGCIDNYLSGRGFELLYAHYYGQEKKAIDIIKANEAGDADAVEHVDRFMELLAICFANL
CCCCHHHHHCCCCCEEEEEEHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
FTCFDPHVVALGGGLSNFALIYDELPKRLPKHLLSVARVPRIIKAKHGDSGGVRGAAFLN
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEEE
IK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA