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The map label for this gene is pbgA [H]

Identifier: 209694294

GI number: 209694294

Start: 791836

End: 793245

Strand: Direct

Name: pbgA [H]

Synonym: VSAL_I0705

Alternate gene names: 209694294

Gene position: 791836-793245 (Clockwise)

Preceding gene: 209694293

Following gene: 209694295

Centisome position: 23.81

GC content: 38.09

Gene sequence:

>1410_bases
ATGAAATTTGCAAATGATTTTTTATTAGGCGCAGCAACGGCTTCTTACCAAATAGAAGGCGCATGGGATCAAGATGGAAA
AGGTCCAACAAACTGGGATGAGTTTACTAAAATTCCAGGGAAAACCTTTGAAGGAACTAATGGCGACATCGCAATTGATC
ATTATAATCGCTATAAAGAAGACATCGCATTAATGGCTGAAATGGGCTTAGAGTCTTATCGCTTTTCAATATCTTGGGCT
CGTATTTTTCCAAATGGCACCGGAGAGATTAATCAAAAAGGAATCGACTTTTACAATAATGTCATTGATGAATGCTTGAA
ATATGGCATCGTTCCTTTTGTTACCTTATATCACTGGGATCTACCTCTTACGTTAGAAAAAGAAGGTTCATGGTTAAATA
AAGCCACCTGTGATGCGTATGTTGAATTTGCAAAGTTATGTTTTAATGAATTTGGAGATCGTGTTAAACATTTCATTACT
TTTAATGAAACCGTTGTGTTTTGCGCATTAGGCTATATGGCAGGCGCACACCCACCAGGGATTCAAAATGATCCTAAGAA
ATATTTTCAAGCGACGCACAACGTATTTTATGCACACGCTAAAGCCGTCATTGAGTATAAAAAACTGAATCAATTTGGTG
AGATTGGCTTGAGTCATGTATTTAGCCCTGCATTTGGGGTCGATGATTCAGAGGCGAGTCACTTTGCCGCAATACATGCG
AACCAATTTAGTATGAATTGGTTCTATGATCCTATCCTATTAGGTAAGTATCCTGAATACGTGGTTAAGCAACTACAAGA
AGAAGGTAACTTGCCCGATTGGACTGATGAAGAACTACAAACTATTTTTGAAGCCGCACCGTTGAATGACTTTATGGGAC
TGAATTACTACCAACCACAACGTGTTGAAAAAATTGAAAGTGGTATGGAAATTCGTAAAATCACTCGTGAAAATTCGACT
GGTTCTCCGGGCAACCCAAGTTACGATAATGTATATCGCACAGTTAAAATGGACGATAAAAAATACACTAAATGGGATTG
GGAAATTTCACCTGAAGGGTTTGTTGCTGGGCTTGAAATGATAAAAGAGCATTATGGTCAGATCAAACTGTACATTACAG
AAAATGGATTGGGTGATGAAGATCCGATTATTGATGGTGAAGTGTGTGATATTCCGCGAATCAATTTTATTAAAGATCAT
CTAGGTGCAGTAAAAGAAGCGGTTCGTCGTGGTGTTCACATTAAGGGGTATTACGCATGGTCAGCGATTGATTTATTGAG
CTGGTTAAACGGTTACAAAAAGCAATATGGGTTCATTTATGTTGATCATCAAAATGGACTAGAGCGTAAGAAAAAAGCGT
CTTTCCATTGGTTTAAAGATGTTATCGCGACTCGTGGTGAAAATATCTAG

Upstream 100 bases:

>100_bases
TACTTTTAAGTTAATCATATTCTAGGCCTAAGAAGACTTAGGCCAATACAAACATCAGTTGGCTATCGATTTGTTAGCAG
CAGTAGAGCAGGAATTCGAG

Downstream 100 bases:

>100_bases
CGTTATATTAGAAATGGGAAAGTCGAATTATGGCAACGTATTTATCGTTTGATATTGGTGGAACAGACATTAAGTTTGGC
GTGTTGAATGAACACGGCCA

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKFANDFLLGAATASYQIEGAWDQDGKGPTNWDEFTKIPGKTFEGTNGDIAIDHYNRYKEDIALMAEMGLESYRFSISWA
RIFPNGTGEINQKGIDFYNNVIDECLKYGIVPFVTLYHWDLPLTLEKEGSWLNKATCDAYVEFAKLCFNEFGDRVKHFIT
FNETVVFCALGYMAGAHPPGIQNDPKKYFQATHNVFYAHAKAVIEYKKLNQFGEIGLSHVFSPAFGVDDSEASHFAAIHA
NQFSMNWFYDPILLGKYPEYVVKQLQEEGNLPDWTDEELQTIFEAAPLNDFMGLNYYQPQRVEKIESGMEIRKITRENST
GSPGNPSYDNVYRTVKMDDKKYTKWDWEISPEGFVAGLEMIKEHYGQIKLYITENGLGDEDPIIDGEVCDIPRINFIKDH
LGAVKEAVRRGVHIKGYYAWSAIDLLSWLNGYKKQYGFIYVDHQNGLERKKKASFHWFKDVIATRGENI

Sequences:

>Translated_469_residues
MKFANDFLLGAATASYQIEGAWDQDGKGPTNWDEFTKIPGKTFEGTNGDIAIDHYNRYKEDIALMAEMGLESYRFSISWA
RIFPNGTGEINQKGIDFYNNVIDECLKYGIVPFVTLYHWDLPLTLEKEGSWLNKATCDAYVEFAKLCFNEFGDRVKHFIT
FNETVVFCALGYMAGAHPPGIQNDPKKYFQATHNVFYAHAKAVIEYKKLNQFGEIGLSHVFSPAFGVDDSEASHFAAIHA
NQFSMNWFYDPILLGKYPEYVVKQLQEEGNLPDWTDEELQTIFEAAPLNDFMGLNYYQPQRVEKIESGMEIRKITRENST
GSPGNPSYDNVYRTVKMDDKKYTKWDWEISPEGFVAGLEMIKEHYGQIKLYITENGLGDEDPIIDGEVCDIPRINFIKDH
LGAVKEAVRRGVHIKGYYAWSAIDLLSWLNGYKKQYGFIYVDHQNGLERKKKASFHWFKDVIATRGENI
>Mature_469_residues
MKFANDFLLGAATASYQIEGAWDQDGKGPTNWDEFTKIPGKTFEGTNGDIAIDHYNRYKEDIALMAEMGLESYRFSISWA
RIFPNGTGEINQKGIDFYNNVIDECLKYGIVPFVTLYHWDLPLTLEKEGSWLNKATCDAYVEFAKLCFNEFGDRVKHFIT
FNETVVFCALGYMAGAHPPGIQNDPKKYFQATHNVFYAHAKAVIEYKKLNQFGEIGLSHVFSPAFGVDDSEASHFAAIHA
NQFSMNWFYDPILLGKYPEYVVKQLQEEGNLPDWTDEELQTIFEAAPLNDFMGLNYYQPQRVEKIESGMEIRKITRENST
GSPGNPSYDNVYRTVKMDDKKYTKWDWEISPEGFVAGLEMIKEHYGQIKLYITENGLGDEDPIIDGEVCDIPRINFIKDH
LGAVKEAVRRGVHIKGYYAWSAIDLLSWLNGYKKQYGFIYVDHQNGLERKKKASFHWFKDVIATRGENI

Specific function: Is able to catalyze the hydrolysis of aryl-phospho-beta- D-glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=463, Percent_Identity=37.1490280777538, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI110681710, Length=485, Percent_Identity=35.4639175257732, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI32481206, Length=486, Percent_Identity=37.2427983539095, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI28376633, Length=474, Percent_Identity=30.168776371308, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI24497614, Length=468, Percent_Identity=29.4871794871795, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI190360571, Length=99, Percent_Identity=48.4848484848485, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI2367174, Length=502, Percent_Identity=34.8605577689243, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI2367270, Length=486, Percent_Identity=33.7448559670782, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1789070, Length=497, Percent_Identity=33.4004024144869, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17539390, Length=464, Percent_Identity=34.051724137931, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17552856, Length=481, Percent_Identity=32.4324324324324, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21356577, Length=489, Percent_Identity=32.1063394683027, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53806; Mature: 53806

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFANDFLLGAATASYQIEGAWDQDGKGPTNWDEFTKIPGKTFEGTNGDIAIDHYNRYKE
CCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHH
DIALMAEMGLESYRFSISWARIFPNGTGEINQKGIDFYNNVIDECLKYGIVPFVTLYHWD
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHEECC
LPLTLEKEGSWLNKATCDAYVEFAKLCFNEFGDRVKHFITFNETVVFCALGYMAGAHPPG
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCC
IQNDPKKYFQATHNVFYAHAKAVIEYKKLNQFGEIGLSHVFSPAFGVDDSEASHFAAIHA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
NQFSMNWFYDPILLGKYPEYVVKQLQEEGNLPDWTDEELQTIFEAAPLNDFMGLNYYQPQ
CEEECCEEECCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCHH
RVEKIESGMEIRKITRENSTGSPGNPSYDNVYRTVKMDDKKYTKWDWEISPEGFVAGLEM
HHHHHHCCHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCEEECCEECCCHHHHHHHH
IKEHYGQIKLYITENGLGDEDPIIDGEVCDIPRINFIKDHLGAVKEAVRRGVHIKGYYAW
HHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHH
SAIDLLSWLNGYKKQYGFIYVDHQNGLERKKKASFHWFKDVIATRGENI
HHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFANDFLLGAATASYQIEGAWDQDGKGPTNWDEFTKIPGKTFEGTNGDIAIDHYNRYKE
CCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEHHHHHHHH
DIALMAEMGLESYRFSISWARIFPNGTGEINQKGIDFYNNVIDECLKYGIVPFVTLYHWD
HHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHEECC
LPLTLEKEGSWLNKATCDAYVEFAKLCFNEFGDRVKHFITFNETVVFCALGYMAGAHPPG
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCC
IQNDPKKYFQATHNVFYAHAKAVIEYKKLNQFGEIGLSHVFSPAFGVDDSEASHFAAIHA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
NQFSMNWFYDPILLGKYPEYVVKQLQEEGNLPDWTDEELQTIFEAAPLNDFMGLNYYQPQ
CEEECCEEECCEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCHH
RVEKIESGMEIRKITRENSTGSPGNPSYDNVYRTVKMDDKKYTKWDWEISPEGFVAGLEM
HHHHHHCCHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCEEECCEECCCHHHHHHHH
IKEHYGQIKLYITENGLGDEDPIIDGEVCDIPRINFIKDHLGAVKEAVRRGVHIKGYYAW
HHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEHH
SAIDLLSWLNGYKKQYGFIYVDHQNGLERKKKASFHWFKDVIATRGENI
HHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704255; 8969502; 9384377; 2841296 [H]