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The map label for this gene is mtfA

Identifier: 209694282

GI number: 209694282

Start: 778223

End: 779314

Strand: Direct

Name: mtfA

Synonym: VSAL_I0693

Alternate gene names: 209694282

Gene position: 778223-779314 (Clockwise)

Preceding gene: 209694281

Following gene: 209694288

Centisome position: 23.4

GC content: 38.64

Gene sequence:

>1092_bases
GTGAAACAAGTAATGTTCTATTGTCGCTCTGGTTTTGAAAAAGAGTGTGCAGGTGAGATCCAAGATAAAGCCACTCAACT
TGAAGTGTTTGGTTTTCCTCGCCTAAAAAGTAACACTGGCTATGTATTATTTGAATGCTATCAGGAAGGCGATGCAGATA
AACTGATTCAACAGATTAAATTTAATGAGCTTATTTTTGCTCGTCAAATGTTTGCTGTTGCAACAGAATTAAGTGACCTA
CCAACAGAAGATCGTATTTCTCCGATCCTTAATGCATTAAGCAGTGTTGAAGGCGTGCCTCATTGTGGTGATATTCGTAT
TGAAACTCCGGATACGAATGAAGCGAAAGAGCTCTTAAAATTCTGTCGTAAATTTACTGTGCCGCTTCGTCAAGCATTAC
GCGGCAAGCAAATGCTAACAGCAAGAGATACTGACCGAATCCCAGTAATGCACGTGTGCTTTATTGAGCCTGGCCATTGT
TATGTTGGTTACTCATATACGAATAATAATTCAAAATTCTTTATGGGTATTCCTCGTTTAAAATTCCCTTCAGATTCACC
AAGTCGTTCTACATTGAAATTGGAAGAAGCGTTTCACGTATTCATTCCAAGAGATGAATGGGATACTCGTTTGGCTTCTG
GTATGTGGGCTGTTGATTTAGGTGCCTGTCCTGGCGGTTGGACTTACCAACTGGTTAAGCGCTCTATGTTTGTTCATGCG
ATTGATAATGGCATGATGGCACAAAGCTTAATGGATACAGGTCAAGTTAAACACCACATGGTTGATGGTTTTAAATTTGA
ACCACAAAGAAAGAACGTAACGTGGATCGTGTGTGACATGATTGAAAAACCTGCACGAGTGGCTCACCTTATGGGTGAAT
GGTTAATTAAAGGTTGGGCTAAAGAAGCACTATTCAACCTTAAGTTACCAATGAAAGGTCGTTACGACGAAGTTCTTCAA
GATATTGAAAACTTAAAAGAATTTTTAAATAAGAATGGCTTTAAATACAAACTTCAAGCGAAGCATTTGTATCATGACCG
TGAAGAAATTACGGTACATATTCAAGCGATTACAAATATCTCTCCACATTAA

Upstream 100 bases:

>100_bases
TTAAGTGGTTTGGGCCAATAACTCCATTAGGTGGACTCTGTTTTATTAGCGGTTGGCTGACGCTGGCTGTTTCAACTTAT
CGTTTGACTGAGGTTTCAAA

Downstream 100 bases:

>100_bases
TTTTGCTATGAGATAAAACGCTCTTGCAAGATAAATGCAAAACGCCCGTAGATCGTCTGATCTACGGGCGTTTTTATTTA
AATACAGTGTTGAATATGAT

Product: RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKSNTGYVLFECYQEGDADKLIQQIKFNELIFARQMFAVATELSDL
PTEDRISPILNALSSVEGVPHCGDIRIETPDTNEAKELLKFCRKFTVPLRQALRGKQMLTARDTDRIPVMHVCFIEPGHC
YVGYSYTNNNSKFFMGIPRLKFPSDSPSRSTLKLEEAFHVFIPRDEWDTRLASGMWAVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPQRKNVTWIVCDMIEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKEFLNKNGFKYKLQAKHLYHDREEITVHIQAITNISPH

Sequences:

>Translated_363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKSNTGYVLFECYQEGDADKLIQQIKFNELIFARQMFAVATELSDL
PTEDRISPILNALSSVEGVPHCGDIRIETPDTNEAKELLKFCRKFTVPLRQALRGKQMLTARDTDRIPVMHVCFIEPGHC
YVGYSYTNNNSKFFMGIPRLKFPSDSPSRSTLKLEEAFHVFIPRDEWDTRLASGMWAVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPQRKNVTWIVCDMIEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKEFLNKNGFKYKLQAKHLYHDREEITVHIQAITNISPH
>Mature_363_residues
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKSNTGYVLFECYQEGDADKLIQQIKFNELIFARQMFAVATELSDL
PTEDRISPILNALSSVEGVPHCGDIRIETPDTNEAKELLKFCRKFTVPLRQALRGKQMLTARDTDRIPVMHVCFIEPGHC
YVGYSYTNNNSKFFMGIPRLKFPSDSPSRSTLKLEEAFHVFIPRDEWDTRLASGMWAVDLGACPGGWTYQLVKRSMFVHA
IDNGMMAQSLMDTGQVKHHMVDGFKFEPQRKNVTWIVCDMIEKPARVAHLMGEWLIKGWAKEALFNLKLPMKGRYDEVLQ
DIENLKEFLNKNGFKYKLQAKHLYHDREEITVHIQAITNISPH

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily

Homologues:

Organism=Escherichia coli, GI1789171, Length=357, Percent_Identity=63.5854341736695, Blast_Score=493, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMM_ALISL (B6EGG2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262210.1
- GeneID:   6986606
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0693
- HOGENOM:   HBG681972
- OMA:   LYCRPGF
- ProtClustDB:   PRK11760
- GO:   GO:0005737
- HAMAP:   MF_01551
- InterPro:   IPR011224
- InterPro:   IPR002877
- PIRSF:   PIRSF028774

Pfam domain/function: PF01728 FtsJ

EC number: NA

Molecular weight: Translated: 41830; Mature: 41830

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKSNTGYVLFECYQEGDADKLIQQIK
CCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHH
FNELIFARQMFAVATELSDLPTEDRISPILNALSSVEGVPHCGDIRIETPDTNEAKELLK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
FCRKFTVPLRQALRGKQMLTARDTDRIPVMHVCFIEPGHCYVGYSYTNNNSKFFMGIPRL
HHHHHCHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCE
KFPSDSPSRSTLKLEEAFHVFIPRDEWDTRLASGMWAVDLGACPGGWTYQLVKRSMFVHA
ECCCCCCCCCEEEEECEEEEEECCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
IDNGMMAQSLMDTGQVKHHMVDGFKFEPQRKNVTWIVCDMIEKPARVAHLMGEWLIKGWA
HCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEHHHCHHHHHHHHHHHHHHHHH
KEALFNLKLPMKGRYDEVLQDIENLKEFLNKNGFKYKLQAKHLYHDREEITVHIQAITNI
HHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEEEECCC
SPH
CCC
>Mature Secondary Structure
MKQVMFYCRSGFEKECAGEIQDKATQLEVFGFPRLKSNTGYVLFECYQEGDADKLIQQIK
CCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHH
FNELIFARQMFAVATELSDLPTEDRISPILNALSSVEGVPHCGDIRIETPDTNEAKELLK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
FCRKFTVPLRQALRGKQMLTARDTDRIPVMHVCFIEPGHCYVGYSYTNNNSKFFMGIPRL
HHHHHCHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEEECCCCEEEEECCCE
KFPSDSPSRSTLKLEEAFHVFIPRDEWDTRLASGMWAVDLGACPGGWTYQLVKRSMFVHA
ECCCCCCCCCEEEEECEEEEEECCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
IDNGMMAQSLMDTGQVKHHMVDGFKFEPQRKNVTWIVCDMIEKPARVAHLMGEWLIKGWA
HCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEEHHHCHHHHHHHHHHHHHHHHH
KEALFNLKLPMKGRYDEVLQDIENLKEFLNKNGFKYKLQAKHLYHDREEITVHIQAITNI
HHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCEEEEEEEECCC
SPH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA