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The map label for this gene is raiA [H]

Identifier: 209694252

GI number: 209694252

Start: 734792

End: 735154

Strand: Reverse

Name: raiA [H]

Synonym: VSAL_I0662

Alternate gene names: 209694252

Gene position: 735154-734792 (Counterclockwise)

Preceding gene: 209694253

Following gene: 209694251

Centisome position: 22.11

GC content: 40.5

Gene sequence:

>363_bases
ATGAAAGTAGAAATTACAGGCAACAACATCGACATCACTCCTGGCATCCGTGAACGTATTGAAGGGAAATTTAAAAAATT
AGAAGAAAAAAGACATCTCGATATTATTGGACGTCATGCTAGCGTAACAAAACGCTCAAATGGTAAGCACAAGGTAGAAG
CATACGTAACAATTGCAGGTGGTAAAGTTGCAGCCTCAGCTGAGCAAGAAGACCTATTTGGTACAATTAGAGAGATGTAC
CAAAAACTAGAACGCCAACTAGATAAGCTACAACATAAAAGCGAAGCTCGTCGAGCGACTCATGCTCAGGCTCCTGTAGT
AGAAGAGCCAGAAGTTGAACTAGAAGACGAATATGAACAATAG

Upstream 100 bases:

>100_bases
CCAGACAAAACACTTCAAAAAGGTGATCTAGATCACTTTAATTTTGCTGAGGATCCGTAATCTGGATACATGCCAAACGG
GGCAACGAGAGGAAAATCAT

Downstream 100 bases:

>100_bases
TCAACTCCACGTTTTCTGAAACAGCGCTCATTTGAGCGCTGTTTTTTCTTGACGCAAACTTACCTCTTCCTTTATTGTGA
ATTAACTCTTAATTTACTAG

Product: ribosome-associated inhibitor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKVEITGNNIDITPGIRERIEGKFKKLEEKRHLDIIGRHASVTKRSNGKHKVEAYVTIAGGKVAASAEQEDLFGTIREMY
QKLERQLDKLQHKSEARRATHAQAPVVEEPEVELEDEYEQ

Sequences:

>Translated_120_residues
MKVEITGNNIDITPGIRERIEGKFKKLEEKRHLDIIGRHASVTKRSNGKHKVEAYVTIAGGKVAASAEQEDLFGTIREMY
QKLERQLDKLQHKSEARRATHAQAPVVEEPEVELEDEYEQ
>Mature_120_residues
MKVEITGNNIDITPGIRERIEGKFKKLEEKRHLDIIGRHASVTKRSNGKHKVEAYVTIAGGKVAASAEQEDLFGTIREMY
QKLERQLDKLQHKSEARRATHAQAPVVEEPEVELEDEYEQ

Specific function: During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation initiation and increase translation accuracy. When normal growth conditions are restored,

COG id: COG1544

COG function: function code J; Ribosome-associated protein Y (PSrp-1)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma(54) modulation protein family [H]

Homologues:

Organism=Escherichia coli, GI1788949, Length=115, Percent_Identity=40, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1789595, Length=98, Percent_Identity=37.7551020408163, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003489 [H]

Pfam domain/function: PF02482 Ribosomal_S30AE [H]

EC number: NA

Molecular weight: Translated: 13722; Mature: 13722

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEITGNNIDITPGIRERIEGKFKKLEEKRHLDIIGRHASVTKRSNGKHKVEAYVTIAG
CEEEECCCCEEECCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEEEEEEEC
GKVAASAEQEDLFGTIREMYQKLERQLDKLQHKSEARRATHAQAPVVEEPEVELEDEYEQ
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVEITGNNIDITPGIRERIEGKFKKLEEKRHLDIIGRHASVTKRSNGKHKVEAYVTIAG
CEEEECCCCEEECCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEEEEEEEC
GKVAASAEQEDLFGTIREMYQKLERQLDKLQHKSEARRATHAQAPVVEEPEVELEDEYEQ
CEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]