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The map label for this gene is gshA

Identifier: 209694234

GI number: 209694234

Start: 716044

End: 717609

Strand: Direct

Name: gshA

Synonym: VSAL_I0643

Alternate gene names: 209694234

Gene position: 716044-717609 (Clockwise)

Preceding gene: 209694233

Following gene: 209694235

Centisome position: 21.53

GC content: 38.31

Gene sequence:

>1566_bases
TTGACCGAATTTACTAACCGATTACAGAAAGTCGCGTCTAATCCCAAAGCCTTTAAAAATACTGGCCGTGGTATTGAAAG
AGAAACACTTCGTTTTACTCTTGATGCTGCACTTTCTTCAAAACCACATCCAGCAGGCGTAGGTTCAGCACTCACTCACA
AATACATCACAACGGATTTTGCTGAGTCATTATTAGAATTTATTACGCCGGTATCTCATGATGTTAAAACGGTATTAAAG
CAATTAGAAGATGTGCATCATTACACGGTCTCTCATATGGGAGAGGAAAAATTGTGGCCGCTGTCTATGCCATGTTTTGT
GAGCAAAGACGATGACATTACTTTAGCGCAGTATGGTAAATCTAATGTTGGTCAATTAAAAACGACTTACCGTGAAGGTT
TAAAGCGCCGTTATGGCAGTGTGATGCAGGTCATTTCTGGTGTTCATTTTAACTTTTCTTTTTCGAATGAGTTTTGGGAC
GAATTACTGGGTGAGCAAACTGAAAAAGATCGTCAAGATTCGGTATCTGATGCTTATTTTGGGTTGATCCGTAATTACTA
CCGCTTTGGGTGGTTGATCCCTTATTTCTTTGGTGCTTCTCCTGCATTGTGTGGTTCTTTTATTCAAGGTCGAGAAACGA
CAATGAATTTTGAATCACTAGGTAAAACATTGTATTTACCTTATGCAACATCATTAAGATTAAGCGATCTTGGTTATACA
AATGATGCTCAAAGTGATTTAAAAATCAGTTTAAATAGTATCGATGAATATATTGAAGGCTTGAATAAAGCGATTCGCAC
TCCATCGGAAGAGTTTGCAAAAATAGGTTTAAAAGAGGGGGATAAACACATCCAATTAAATGCGAATGTGTTGCAGATTG
AAAATGAATTGTATGCTCCAATTCGCCCTAAGCGTGTCGCTAAATCAGGTGAAAAGCCTTCAGAAGCATTAGAGCGTTCG
GGAATTGAATATATAGAAGTTCGATCTTTAGATGTAAACCCATTTAGCCCTATTGGTGTAGATGAAGATCAAGTTCGATT
CTTAGACCTTTTCTTAACATGGTCTGTACTCACTGATTCTGCTCCTATGGATGACAGTGAAATGGCGTGTTGGAAAGACA
ATTGGAATAAGATTGTTGAACTGGGACGTAAGCCTGGGTTAGAGCTTCAAATTGGTTGCCAAGGGGAGCGTTTAACTCAG
AAAGCATGGGCTGAACGAGTTTTTGATGATCTATTCACAATAGCTAAAAAAATGGATGCAGTTAATGGTGATGATGCATA
CCAACAAACTCATCAACGACTAAGTGCGATGATAGAGAATCCAGAGTTAACTATTTCAGGTCGTCTACTTGCTGAAACGA
AGAAGGCTGGTGGTATTGGTATTATTGGTTGTAAATTGGCAATACAACATCGCCAAGCGCATTTAGATCATAAATACAGC
TTCTATACTAAAGATGAGTTGGATGCAGAGGTAGAGCGTTCTGTTCTGGCTCAGAAAGAAATTGAGGCCAGTGATACAAT
GCCTTTTTCAGAGTATTTAGATGATTATTTCAATTATTTGAAATAA

Upstream 100 bases:

>100_bases
ATACTAATTACTGCTCTTTTTTTATTATTCAAACACGAGTAAAATTTGCTACTATCCTAGGATAAAGATCTTAGATCTAC
CTCAATAGCGAGAATGTATT

Downstream 100 bases:

>100_bases
AACAAATATCGTATAACAAGCACTGTTATCCTAAATGAATAGCAGTGCTTTTTTATTGAGTAAAAAATGAATTATAAATT
TTCACTAATTATTGTCGCCT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MTEFTNRLQKVASNPKAFKNTGRGIERETLRFTLDAALSSKPHPAGVGSALTHKYITTDFAESLLEFITPVSHDVKTVLK
QLEDVHHYTVSHMGEEKLWPLSMPCFVSKDDDITLAQYGKSNVGQLKTTYREGLKRRYGSVMQVISGVHFNFSFSNEFWD
ELLGEQTEKDRQDSVSDAYFGLIRNYYRFGWLIPYFFGASPALCGSFIQGRETTMNFESLGKTLYLPYATSLRLSDLGYT
NDAQSDLKISLNSIDEYIEGLNKAIRTPSEEFAKIGLKEGDKHIQLNANVLQIENELYAPIRPKRVAKSGEKPSEALERS
GIEYIEVRSLDVNPFSPIGVDEDQVRFLDLFLTWSVLTDSAPMDDSEMACWKDNWNKIVELGRKPGLELQIGCQGERLTQ
KAWAERVFDDLFTIAKKMDAVNGDDAYQQTHQRLSAMIENPELTISGRLLAETKKAGGIGIIGCKLAIQHRQAHLDHKYS
FYTKDELDAEVERSVLAQKEIEASDTMPFSEYLDDYFNYLK

Sequences:

>Translated_521_residues
MTEFTNRLQKVASNPKAFKNTGRGIERETLRFTLDAALSSKPHPAGVGSALTHKYITTDFAESLLEFITPVSHDVKTVLK
QLEDVHHYTVSHMGEEKLWPLSMPCFVSKDDDITLAQYGKSNVGQLKTTYREGLKRRYGSVMQVISGVHFNFSFSNEFWD
ELLGEQTEKDRQDSVSDAYFGLIRNYYRFGWLIPYFFGASPALCGSFIQGRETTMNFESLGKTLYLPYATSLRLSDLGYT
NDAQSDLKISLNSIDEYIEGLNKAIRTPSEEFAKIGLKEGDKHIQLNANVLQIENELYAPIRPKRVAKSGEKPSEALERS
GIEYIEVRSLDVNPFSPIGVDEDQVRFLDLFLTWSVLTDSAPMDDSEMACWKDNWNKIVELGRKPGLELQIGCQGERLTQ
KAWAERVFDDLFTIAKKMDAVNGDDAYQQTHQRLSAMIENPELTISGRLLAETKKAGGIGIIGCKLAIQHRQAHLDHKYS
FYTKDELDAEVERSVLAQKEIEASDTMPFSEYLDDYFNYLK
>Mature_520_residues
TEFTNRLQKVASNPKAFKNTGRGIERETLRFTLDAALSSKPHPAGVGSALTHKYITTDFAESLLEFITPVSHDVKTVLKQ
LEDVHHYTVSHMGEEKLWPLSMPCFVSKDDDITLAQYGKSNVGQLKTTYREGLKRRYGSVMQVISGVHFNFSFSNEFWDE
LLGEQTEKDRQDSVSDAYFGLIRNYYRFGWLIPYFFGASPALCGSFIQGRETTMNFESLGKTLYLPYATSLRLSDLGYTN
DAQSDLKISLNSIDEYIEGLNKAIRTPSEEFAKIGLKEGDKHIQLNANVLQIENELYAPIRPKRVAKSGEKPSEALERSG
IEYIEVRSLDVNPFSPIGVDEDQVRFLDLFLTWSVLTDSAPMDDSEMACWKDNWNKIVELGRKPGLELQIGCQGERLTQK
AWAERVFDDLFTIAKKMDAVNGDDAYQQTHQRLSAMIENPELTISGRLLAETKKAGGIGIIGCKLAIQHRQAHLDHKYSF
YTKDELDAEVERSVLAQKEIEASDTMPFSEYLDDYFNYLK

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=517, Percent_Identity=52.6112185686654, Blast_Score=573, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_ALISL (B6EGB3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262162.1
- GeneID:   6986581
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0643
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 59104; Mature: 58973

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFTNRLQKVASNPKAFKNTGRGIERETLRFTLDAALSSKPHPAGVGSALTHKYITTDF
CCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHH
AESLLEFITPVSHDVKTVLKQLEDVHHYTVSHMGEEKLWPLSMPCFVSKDDDITLAQYGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEHHHCCC
SNVGQLKTTYREGLKRRYGSVMQVISGVHFNFSFSNEFWDELLGEQTEKDRQDSVSDAYF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHHHHH
GLIRNYYRFGWLIPYFFGASPALCGSFIQGRETTMNFESLGKTLYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NDAQSDLKISLNSIDEYIEGLNKAIRTPSEEFAKIGLKEGDKHIQLNANVLQIENELYAP
CCCCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCEEEEECCHHCCC
IRPKRVAKSGEKPSEALERSGIEYIEVRSLDVNPFSPIGVDEDQVRFLDLFLTWSVLTDS
CCHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
APMDDSEMACWKDNWNKIVELGRKPGLELQIGCQGERLTQKAWAERVFDDLFTIAKKMDA
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
VNGDDAYQQTHQRLSAMIENPELTISGRLLAETKKAGGIGIIGCKLAIQHRQAHLDHKYS
CCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEE
FYTKDELDAEVERSVLAQKEIEASDTMPFSEYLDDYFNYLK
ECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFTNRLQKVASNPKAFKNTGRGIERETLRFTLDAALSSKPHPAGVGSALTHKYITTDF
CHHHHHHHHHHCCCHHHHHCCCCCCHHHHHEEHHHHHCCCCCCCCCCHHHHHHHHHHHH
AESLLEFITPVSHDVKTVLKQLEDVHHYTVSHMGEEKLWPLSMPCFVSKDDDITLAQYGK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCEEHHHCCC
SNVGQLKTTYREGLKRRYGSVMQVISGVHFNFSFSNEFWDELLGEQTEKDRQDSVSDAYF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHHHHH
GLIRNYYRFGWLIPYFFGASPALCGSFIQGRETTMNFESLGKTLYLPYATSLRLSDLGYT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCEEECCCCCC
NDAQSDLKISLNSIDEYIEGLNKAIRTPSEEFAKIGLKEGDKHIQLNANVLQIENELYAP
CCCCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCEEEEECCHHCCC
IRPKRVAKSGEKPSEALERSGIEYIEVRSLDVNPFSPIGVDEDQVRFLDLFLTWSVLTDS
CCHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
APMDDSEMACWKDNWNKIVELGRKPGLELQIGCQGERLTQKAWAERVFDDLFTIAKKMDA
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHC
VNGDDAYQQTHQRLSAMIENPELTISGRLLAETKKAGGIGIIGCKLAIQHRQAHLDHKYS
CCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEE
FYTKDELDAEVERSVLAQKEIEASDTMPFSEYLDDYFNYLK
ECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA