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The map label for this gene is rrmJ

Identifier: 209694183

GI number: 209694183

Start: 652081

End: 652710

Strand: Direct

Name: rrmJ

Synonym: VSAL_I0591

Alternate gene names: 209694183

Gene position: 652081-652710 (Clockwise)

Preceding gene: 209694181

Following gene: 209694184

Centisome position: 19.61

GC content: 41.11

Gene sequence:

>630_bases
ATGAGTAAGAATAAACTTTCAGCAAGCTCTGGCCGCTGGTTAAAAGAACATTTTGATGATAAATACGTTCTTGAAGCGCA
AAAAAGAGGCTATCGCTCTCGTGCTATTTTTAAACTTGAAGAAATTCAAAATAAAGACAAGCTATTAAAGCCTGGAATGA
CGGTGGTTGACCTTGGTGCTGCTCCTGGTGGGTGGTCACAATATGCGGTTGAGCAAGTCGGTGATTCTGGTCAAGTAATC
GCTTGTGATATTTTAGCTATGGACTCAATTGCGGGTGTGAGCTTTTTACAAGGGGATTTTAGAGAAGAAGCGGTACTAGA
CGCATTGCTTGAACGCATACAACCAGATATGGTAGATGTAGTAATGTCGGATATGGCGCCAAATATGAGTGGTAACCCTT
CGGTAGATCAACCAAGATCAATGTATCTTGTTGAGTTAGCGTTGGATATGTGCCGCCAAGTATTGGCTCCTAATGGCAGT
TTCACAGTTAAAGTATTCCAAGGTGAAAGCTTTGATGCATACTTACAAGAAGTACGTAAGGTATTCAAAGTCGTTAAGAT
TAGAAAACCTGATTCATCACGAGCTCGCTCTCGTGAAGTGTATATTGTAGCCACTGGTTACAAAGGATAA

Upstream 100 bases:

>100_bases
ATATTAAGGGTTGAAAACAGCCTATTCTAACGCCATCTATCCCTGAAGACTATTTTTTGTTTCAGTCTTCTAATTTAAAT
TAAGTATTTTTAGGTAATGC

Downstream 100 bases:

>100_bases
TTCTTACGTAGTAGCTACAGGATATAAACTGTAGTACTCTTTCATTCATCTATTTAGTTAACGCGGGGTTAACACCTTGA
GTGACATGGCAAAAAATCTA

Product: 23S rRNA methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSKNKLSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKLEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDSGQVI
ACDILAMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNPSVDQPRSMYLVELALDMCRQVLAPNGS
FTVKVFQGESFDAYLQEVRKVFKVVKIRKPDSSRARSREVYIVATGYKG

Sequences:

>Translated_209_residues
MSKNKLSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKLEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDSGQVI
ACDILAMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNPSVDQPRSMYLVELALDMCRQVLAPNGS
FTVKVFQGESFDAYLQEVRKVFKVVKIRKPDSSRARSREVYIVATGYKG
>Mature_208_residues
SKNKLSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKLEEIQNKDKLLKPGMTVVDLGAAPGGWSQYAVEQVGDSGQVIA
CDILAMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDVVMSDMAPNMSGNPSVDQPRSMYLVELALDMCRQVLAPNGSF
TVKVFQGESFDAYLQEVRKVFKVVKIRKPDSSRARSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=35.0230414746544, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI29029591, Length=199, Percent_Identity=34.1708542713568, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29029589, Length=199, Percent_Identity=34.1708542713568, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7110661, Length=199, Percent_Identity=34.1708542713568, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=32.9787234042553, Blast_Score=110, Evalue=9e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=73.0769230769231, Blast_Score=317, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=32.2727272727273, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=31.413612565445, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=31.8181818181818, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=210, Percent_Identity=30, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=37.5, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=34.9056603773585, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=36.1702127659575, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=30.3030303030303, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648639, Length=202, Percent_Identity=31.1881188118812, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ALISL (B6END5)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262111.1
- GeneID:   6986557
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0591
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   PRK11188
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23280; Mature: 23149

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: ACT_SITE 164-164 BINDING 63-63 BINDING 65-65 BINDING 83-83 BINDING 99-99 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNKLSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKLEEIQNKDKLLKPGMTVVDLGA
CCCCCCCCCCCCHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEECCC
APGGWSQYAVEQVGDSGQVIACDILAMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDV
CCCCHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMSGNPSVDQPRSMYLVELALDMCRQVLAPNGSFTVKVFQGESFDAYLQEVRK
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
VFKVVKIRKPDSSRARSREVYIVATGYKG
HHHHHHCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SKNKLSASSGRWLKEHFDDKYVLEAQKRGYRSRAIFKLEEIQNKDKLLKPGMTVVDLGA
CCCCCCCCCCCHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCEEEECCC
APGGWSQYAVEQVGDSGQVIACDILAMDSIAGVSFLQGDFREEAVLDALLERIQPDMVDV
CCCCHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VMSDMAPNMSGNPSVDQPRSMYLVELALDMCRQVLAPNGSFTVKVFQGESFDAYLQEVRK
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHH
VFKVVKIRKPDSSRARSREVYIVATGYKG
HHHHHHCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA