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The map label for this gene is carA [H]

Identifier: 209694177

GI number: 209694177

Start: 643459

End: 644613

Strand: Direct

Name: carA [H]

Synonym: VSAL_I0585

Alternate gene names: 209694177

Gene position: 643459-644613 (Clockwise)

Preceding gene: 209694176

Following gene: 209694178

Centisome position: 19.35

GC content: 44.42

Gene sequence:

>1155_bases
TTGAGTAAATCAGCGCTATTAGTCCTAGAAGATGGGACAGTGTTCCGCGGTCAATCGATTGGAGCAGATGGCTCGGCCGT
CGGTGAAGTCGTTTTTAATACCTCGATGACGGGGTACCAAGAGATTCTCACTGATCCTTCCTATTCTCAACAAATCGTTA
CTCTTACTTATCCTCACATTGGCAACACCGGAATCAATTCCGAAGATGAAGAATCTTCTTCTATCCACGCTCAAGGCCTT
GTGATTCGCGATCTTCCTCTCCTAGCTTCAAACTTCCGCAGTGAACAAACTCTTTCTGAATACCTCAAATCTCAAAATAT
TGTCGGCATTGCTGATATCGATACGCGTAAGCTGACTCGTATTTTGCGTGAGAAGGGTGCTCAAAACGGTTGTATCATTG
CTTGCTCTTCTAAGAAAGAAAGCAATTTAGATGAAGCATTTGCTCTTGCTCAAGCAAAAGCATTCCCGGGTCTTAAAGGA
ATGGATTTAGCGAAGGAAGTTACCACGTCAGAAGCTTACCAGTGGAAACAAGGTTCTTGGACTCTTACTGGCGGTTTGCC
AGAAGAAAAAGCAGACAGCGAACTACCGTTCCATGTTGTTGCTTATGATTTTGGTGCTAAACGTAATATCTTACGTATGT
TGGTTGACCGCGGTTGTCGTCTAACGGTCGTTCCGGCACAAACTTCAGCAGAAGAAGTATTAGCGATGAACCCAGATGGT
GTGTTCTTATCAAACGGTCCTGGTGACCCGGCGCCATGTACTTACGCTATTGAAGCAACGAAAGTGTTCTTAGACAAAGG
GTTACCAATCTTTGGTATCTGTCTTGGTCACCAAATTCTTGCTCTAGCATCAGGCGCTCAAACAATTAAGATGAAGTTTG
GCCATCACGGTGCAAACCACCCTGTTAAAGATTTGGATCGTGATGTGGTTATGATTACTGCACAGAACCATGGTTTTGCA
GCAGATGAAGAAACACTTCCTGCAAATCTACGTGCAACGCACAAATCATTGTTTGATGGCACACTTCAAGGCATTCATCG
TACAGATAAGCCTGCATTTAGCTTCCAAGGTCACCCTGAAGCAAGCCCTGGCCCACATGATGCAGCGCCCTTGTTTAACC
ATTTCATTGAATTGATTCAGCAGCATAAAGCTTAA

Upstream 100 bases:

>100_bases
TGTCAAAATTCGAGATTTAATTTCGATTTTTGGGTTTAAAAAAAGAAGTTTTGCATTTTTATGTATGCATGCTGAATGTT
TATTATGTTTGGAGGTTGTC

Downstream 100 bases:

>100_bases
TTCGGAGTAGTAATAAGATGCCAAAACGTACTGACATTAAAAGCGTTCTAATTCTAGGTGCAGGCCCAATCGTAATCGGC
CAAGCATGTGAATTTGACTA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSKSALLVLEDGTVFRGQSIGADGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGINSEDEESSSIHAQGL
VIRDLPLLASNFRSEQTLSEYLKSQNIVGIADIDTRKLTRILREKGAQNGCIIACSSKKESNLDEAFALAQAKAFPGLKG
MDLAKEVTTSEAYQWKQGSWTLTGGLPEEKADSELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEEVLAMNPDG
VFLSNGPGDPAPCTYAIEATKVFLDKGLPIFGICLGHQILALASGAQTIKMKFGHHGANHPVKDLDRDVVMITAQNHGFA
ADEETLPANLRATHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFNHFIELIQQHKA

Sequences:

>Translated_384_residues
MSKSALLVLEDGTVFRGQSIGADGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGINSEDEESSSIHAQGL
VIRDLPLLASNFRSEQTLSEYLKSQNIVGIADIDTRKLTRILREKGAQNGCIIACSSKKESNLDEAFALAQAKAFPGLKG
MDLAKEVTTSEAYQWKQGSWTLTGGLPEEKADSELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEEVLAMNPDG
VFLSNGPGDPAPCTYAIEATKVFLDKGLPIFGICLGHQILALASGAQTIKMKFGHHGANHPVKDLDRDVVMITAQNHGFA
ADEETLPANLRATHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFNHFIELIQQHKA
>Mature_383_residues
SKSALLVLEDGTVFRGQSIGADGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGINSEDEESSSIHAQGLV
IRDLPLLASNFRSEQTLSEYLKSQNIVGIADIDTRKLTRILREKGAQNGCIIACSSKKESNLDEAFALAQAKAFPGLKGM
DLAKEVTTSEAYQWKQGSWTLTGGLPEEKADSELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEEVLAMNPDGV
FLSNGPGDPAPCTYAIEATKVFLDKGLPIFGICLGHQILALASGAQTIKMKFGHHGANHPVKDLDRDVVMITAQNHGFAA
DEETLPANLRATHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFNHFIELIQQHKA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=391, Percent_Identity=39.1304347826087, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI169790915, Length=395, Percent_Identity=35.6962025316456, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI21361331, Length=395, Percent_Identity=35.6962025316456, Blast_Score=221, Evalue=8e-58,
Organism=Escherichia coli, GI1786215, Length=383, Percent_Identity=80.9399477806788, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=393, Percent_Identity=36.3867684478371, Blast_Score=231, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=397, Percent_Identity=37.5314861460957, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=35.5163727959698, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45555749, Length=399, Percent_Identity=38.0952380952381, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24642586, Length=399, Percent_Identity=38.0952380952381, Blast_Score=231, Evalue=9e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41495; Mature: 41364

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSALLVLEDGTVFRGQSIGADGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEECCCCCCCCHHHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGINSEDEESSSIHAQGLVIRDLPLLASNFRSEQTLSEYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHH
ILREKGAQNGCIIACSSKKESNLDEAFALAQAKAFPGLKGMDLAKEVTTSEAYQWKQGSW
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCE
TLTGGLPEEKADSELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEEVLAMNPDG
EEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCC
VFLSNGPGDPAPCTYAIEATKVFLDKGLPIFGICLGHQILALASGAQTIKMKFGHHGANH
EEEECCCCCCCCEEEEEHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
PVKDLDRDVVMITAQNHGFAADEETLPANLRATHKSLFDGTLQGIHRTDKPAFSFQGHPE
CHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ASPGPHDAAPLFNHFIELIQQHKA
CCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSALLVLEDGTVFRGQSIGADGSAVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCEEEEEECCCEEECCCCCCCCHHHHHEEECCCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGINSEDEESSSIHAQGLVIRDLPLLASNFRSEQTLSEYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHHCCCEEEECCCHHHHHH
ILREKGAQNGCIIACSSKKESNLDEAFALAQAKAFPGLKGMDLAKEVTTSEAYQWKQGSW
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCE
TLTGGLPEEKADSELPFHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEEVLAMNPDG
EEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCC
VFLSNGPGDPAPCTYAIEATKVFLDKGLPIFGICLGHQILALASGAQTIKMKFGHHGANH
EEEECCCCCCCCEEEEEHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCC
PVKDLDRDVVMITAQNHGFAADEETLPANLRATHKSLFDGTLQGIHRTDKPAFSFQGHPE
CHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ASPGPHDAAPLFNHFIELIQQHKA
CCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA