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The map label for this gene is trmB

Identifier: 209694128

GI number: 209694128

Start: 591105

End: 591821

Strand: Direct

Name: trmB

Synonym: VSAL_I0535

Alternate gene names: 209694128

Gene position: 591105-591821 (Clockwise)

Preceding gene: 209694121

Following gene: 209694129

Centisome position: 17.78

GC content: 45.89

Gene sequence:

>717_bases
ATGACTGAAGTGACTAAAAACGACTTTACCGAAGAAGGTAAAATTGTTCGTAAAATTCGCAGCTTTGTACGCCGCGAAGG
ACGTTTAACGAAAGGCCAAGAAGGCGCTATTACTGAGTGCTGGTCTACCATGGGCATCGATTTTGTTGAGCAGGTGCTTG
ATTGGAACGAGGTGTTTGGTAACAACAACCCTGTCGTTTTAGAAATCGGTTTTGGTATGGGTGCATCTTTGGTTGAGATG
GCGCAAAAAGCACCAGACGTTAATTTCCTTGGTATTGAAGTTCACCGCCCAGGTGTTGGTGCATGTCTTGCTGCTGCAAA
AGAAGCGGGCGTAACTAACCTACGCGTGATGTGTCACGATGCGGTTGAAGTGTTTGAATCGATGATCCCTGATTCATCAG
TGACGACTCTGCAATTATTCTTCCCTGACCCATGGCATAAAGCGCGTCATCATAAGCGCCGCATCGTTAAAGCTGAATTT
GCTGAGATGATCCGTCCTAAGCTCAATGCTGAAGGTGTTTTTCACATGGCAACAGATTGGGAAAACTACGCAGAACATAT
GATCGAAGTAATGAACGCGGCCCCAGGCTACGAAAACATAGCAACTGATGGCGATTACATTCCTCGTCCAGATGAGCGTC
CATTAACAAAATTTGAAGCTCGTGGTCACCGTTTAGGCCATGGCGTATGGGATATTAAATTCCGTCGTAACGCATAA

Upstream 100 bases:

>100_bases
TTTATACGGAAAACGAAAGATAATGCTTGAACACTGGGCTAAACTTTGGATAATGCCGGATCCACAATCCTTGTTCAGAG
AATAACCAGGTTATCCAATC

Downstream 100 bases:

>100_bases
TTATAATCAGTAGTAAACTGATGCAGTGTCTAATACATTGCTTATAAATTTAAAGCCAACCTCTGTGTTGGCTTTTTTGT
CCTTGCTGTGAAAGGAAGTA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAITECWSTMGIDFVEQVLDWNEVFGNNNPVVLEIGFGMGASLVEM
AQKAPDVNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVTTLQLFFPDPWHKARHHKRRIVKAEF
AEMIRPKLNAEGVFHMATDWENYAEHMIEVMNAAPGYENIATDGDYIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA

Sequences:

>Translated_238_residues
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAITECWSTMGIDFVEQVLDWNEVFGNNNPVVLEIGFGMGASLVEM
AQKAPDVNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVTTLQLFFPDPWHKARHHKRRIVKAEF
AEMIRPKLNAEGVFHMATDWENYAEHMIEVMNAAPGYENIATDGDYIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
>Mature_237_residues
TEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAITECWSTMGIDFVEQVLDWNEVFGNNNPVVLEIGFGMGASLVEMA
QKAPDVNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVTTLQLFFPDPWHKARHHKRRIVKAEFA
EMIRPKLNAEGVFHMATDWENYAEHMIEVMNAAPGYENIATDGDYIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=235, Percent_Identity=63.8297872340426, Blast_Score=330, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_ALISL (B6EMU1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262056.1
- ProteinModelPortal:   B6EMU1
- GeneID:   6988872
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0535
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26925; Mature: 26794

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: BINDING 68-68 BINDING 93-93 BINDING 120-120 BINDING 143-143 BINDING 147-147 BINDING 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAITECWSTMGIDFVEQVLDWNEVFG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
NNNPVVLEIGFGMGASLVEMAQKAPDVNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
AVEVFESMIPDSSVTTLQLFFPDPWHKARHHKRRIVKAEFAEMIRPKLNAEGVFHMATDW
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCH
ENYAEHMIEVMNAAPGYENIATDGDYIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAITECWSTMGIDFVEQVLDWNEVFG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
NNNPVVLEIGFGMGASLVEMAQKAPDVNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
AVEVFESMIPDSSVTTLQLFFPDPWHKARHHKRRIVKAEFAEMIRPKLNAEGVFHMATDW
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCH
ENYAEHMIEVMNAAPGYENIATDGDYIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA