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The map label for this gene is ptsN [H]

Identifier: 209694091

GI number: 209694091

Start: 556789

End: 557235

Strand: Reverse

Name: ptsN [H]

Synonym: VSAL_I0496

Alternate gene names: 209694091

Gene position: 557235-556789 (Counterclockwise)

Preceding gene: 209694092

Following gene: 209694090

Centisome position: 16.76

GC content: 40.49

Gene sequence:

>447_bases
ATGCAGCTAAGCACCATTCTTTCATTGGATTGCACCAAAAGTGCAGTCCAGTGTACTAGCAAGAAACGCGCACTAGAATT
GATCAGTGAAATTGCAGCACCTCAACTGGGTCAAAATCCACAAGCGCTTTTTGAAAGCTTATTAAATCGTGAAAAAATGG
GAAGTACAGGCATCGGCAATGGCATTGCTATCCCCCACGGTCGACTTCATAATTGTGATACTGCGGTAGCGGTATTAATC
CAATGCCAAGAACCTATAGAGTTTGATTCTATTGATAATCGTCCTGTTGATGTTCTTTTTGCTTTACTTGTTCCTGATGA
ACAATGCAAATTACACCTCAAAACACTGTCTAGTGTGGCTGAAAAACTCAATGACAAACAGATCTTACGTCAGCTTCGTC
ATGCTGAAAGCGATCAAGAGTTGTATCAAATTTTCACATCTGAATAG

Upstream 100 bases:

>100_bases
CATTCTGATTCAGAAGATATGTATGCCGCGATTGATGGCTTGGTGGATAAACTTATTCGCCAACTCAATAAACATAAAGA
AAAATTAAGTAGTCATTAAC

Downstream 100 bases:

>100_bases
ATTAAAAAGTTAAGGTTATAAACATGAAGCTAATGGTCGTTAGTGGTAATTCAGGCGCCGGTAAATCGGTTGCCCTGCGA
GTGTTAGAAGATTTAGGTTA

Product: nitrogen regulatory IIA protein, PTS system

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; PTS system EIIA component; Phosphotransferase enzyme IIA component [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPQLGQNPQALFESLLNREKMGSTGIGNGIAIPHGRLHNCDTAVAVLI
QCQEPIEFDSIDNRPVDVLFALLVPDEQCKLHLKTLSSVAEKLNDKQILRQLRHAESDQELYQIFTSE

Sequences:

>Translated_148_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPQLGQNPQALFESLLNREKMGSTGIGNGIAIPHGRLHNCDTAVAVLI
QCQEPIEFDSIDNRPVDVLFALLVPDEQCKLHLKTLSSVAEKLNDKQILRQLRHAESDQELYQIFTSE
>Mature_148_residues
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPQLGQNPQALFESLLNREKMGSTGIGNGIAIPHGRLHNCDTAVAVLI
QCQEPIEFDSIDNRPVDVLFALLVPDEQCKLHLKTLSSVAEKLNDKQILRQLRHAESDQELYQIFTSE

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=64.1891891891892, Blast_Score=189, Evalue=6e-50,
Organism=Escherichia coli, GI1786951, Length=144, Percent_Identity=25.6944444444444, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16397; Mature: 16397

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPQLGQNPQALFESLLNREKMGSTGIGN
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLHNCDTAVAVLIQCQEPIEFDSIDNRPVDVLFALLVPDEQCKLHLKTLSSVA
CEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
EKLNDKQILRQLRHAESDQELYQIFTSE
HHCCHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MQLSTILSLDCTKSAVQCTSKKRALELISEIAAPQLGQNPQALFESLLNREKMGSTGIGN
CCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGRLHNCDTAVAVLIQCQEPIEFDSIDNRPVDVLFALLVPDEQCKLHLKTLSSVA
CEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
EKLNDKQILRQLRHAESDQELYQIFTSE
HHCCHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7876255; 8444818; 8025669; 9278503; 9636714 [H]