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The map label for this gene is pmbA [H]

Identifier: 209694087

GI number: 209694087

Start: 552536

End: 553879

Strand: Direct

Name: pmbA [H]

Synonym: VSAL_I0492

Alternate gene names: 209694087

Gene position: 552536-553879 (Clockwise)

Preceding gene: 209694085

Following gene: 209694100

Centisome position: 16.62

GC content: 42.49

Gene sequence:

>1344_bases
ATGGATGCAAAAGCCCAAATGATGAATCAAAAAATAGAACTTGAAGCAGCAGTAGCGAAGGCTTTAGAAATGGCAACTAA
GCATGCGAGTGCTGCAGAAGTTGCGATTACAAAAACGACAGGAATCAGTGTGTCAACTTTGATGGGAGAGGTTGAAAACG
TTGAGTTTAATCGTGATGGCGCATTAGGTATTACGGTTTACCGTGGCCATAGAAAAGGCAGCGCATCGACATCTGATTTA
AGTGACAAAGCCATTCAAGAGACGGTAGCAGCTGCGCTTGATATCGCAAATTATACGTCAGAAGACCCATTTTCAGGTCC
GGCACCAAAAGAATTAATGGCGCAAAATATTCCTGATTTAGATTTGTTTCATCCTGATGATCCAGAGCCAGACTATGCCG
CACAAAAGGCAATTGAAGCTGAAGAAGCCGCTTTAGCTTATCATTCATCCATTAAGCAAAGTGATGGAGCGAGCTACGAT
AGCCATTATGGTATTAAGGTTTATGGTAATAGTCATGGCATGTTAGCGAGCTATCCATCAAGTCGCCACAGTATTAGCTG
CAGTGTCATTGGTGTTGATAAAAATGGCAACATGGAGCGAGATTACAGCTACACCGTTGCTCGCCATCGTGATGATTTAT
GGGATGCGAAAAAAGTAGGCTTAGACGCTGCTGAGCGAACTGTCAGCCGTTTAGATGCTCAAAAATTAAATACGATGAAA
GTACCGGTTATGTTTTCAGCTGATGTTGCAACGGGCTTAATTGGGCATCTTGTGATGGCGATTAGCGGAGCAAACCTTTA
TCGTAAATCAACGTTTATGCTTGATAAGCTAAATGAAAAAATCTTACCAGATTGGTTTAACGTGAGTGAACGCCCACATA
TATTGAAAGGTCTAGCAAGCAGTCCATTTGATAGCGAAGGGCTGTATACACAAGATCGTGAAATAATCACTGATGGCGTA
TTAGCGACGTATTTACTAACGAGCTACGCTGCACGAAAATTGAATATGACACCAACGGGTCACGCTGGTGGTATTCATAA
CTGGTTTGTTAAATCAACAGGCCAAAGTTTTGATCAAATGCTCAAAACAATGGGAACGGGTCTTCTTGTTACTGAGCTTA
TGGGCCAAGGCGTAAATACGGTGACGGGTGATTACTCTCGTGGTGCCGCTGGTTTTTGGGTTGAGAATGGTGAGATCCAA
TATCCTGTGTCAGAGATTACCATTGCGGGCAACTTAGCAGAAATGTACCAAAACATTATTGCAGTCGGTAGTGATGTAGA
AACACGTTCACAAATTCAAACGGGTTCAATCCTAATTGAATCGATGAAAATAGCCGGTCAGTAA

Upstream 100 bases:

>100_bases
GGTGATTCTCTCTATTTTAGCAAGATAAATAGATAATTGGGCGCAAATTTATCACATAGAGTGTTATTCTAGTAAAAATG
TATATGTTCAGTCAGATGTT

Downstream 100 bases:

>100_bases
TACTTGAATCGTTTTATAAAAAGAATAAAAAAGCGCGCTGTCATATTATGAGAGCGCGCTTTTTAATGCGTTATTTTTAT
GTATTACATTAAAATAGTGG

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MDAKAQMMNQKIELEAAVAKALEMATKHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDL
SDKAIQETVAAALDIANYTSEDPFSGPAPKELMAQNIPDLDLFHPDDPEPDYAAQKAIEAEEAALAYHSSIKQSDGASYD
SHYGIKVYGNSHGMLASYPSSRHSISCSVIGVDKNGNMERDYSYTVARHRDDLWDAKKVGLDAAERTVSRLDAQKLNTMK
VPVMFSADVATGLIGHLVMAISGANLYRKSTFMLDKLNEKILPDWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGV
LATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFDQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQ
YPVSEITIAGNLAEMYQNIIAVGSDVETRSQIQTGSILIESMKIAGQ

Sequences:

>Translated_447_residues
MDAKAQMMNQKIELEAAVAKALEMATKHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDL
SDKAIQETVAAALDIANYTSEDPFSGPAPKELMAQNIPDLDLFHPDDPEPDYAAQKAIEAEEAALAYHSSIKQSDGASYD
SHYGIKVYGNSHGMLASYPSSRHSISCSVIGVDKNGNMERDYSYTVARHRDDLWDAKKVGLDAAERTVSRLDAQKLNTMK
VPVMFSADVATGLIGHLVMAISGANLYRKSTFMLDKLNEKILPDWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGV
LATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFDQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQ
YPVSEITIAGNLAEMYQNIIAVGSDVETRSQIQTGSILIESMKIAGQ
>Mature_447_residues
MDAKAQMMNQKIELEAAVAKALEMATKHASAAEVAITKTTGISVSTLMGEVENVEFNRDGALGITVYRGHRKGSASTSDL
SDKAIQETVAAALDIANYTSEDPFSGPAPKELMAQNIPDLDLFHPDDPEPDYAAQKAIEAEEAALAYHSSIKQSDGASYD
SHYGIKVYGNSHGMLASYPSSRHSISCSVIGVDKNGNMERDYSYTVARHRDDLWDAKKVGLDAAERTVSRLDAQKLNTMK
VPVMFSADVATGLIGHLVMAISGANLYRKSTFMLDKLNEKILPDWFNVSERPHILKGLASSPFDSEGLYTQDREIITDGV
LATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFDQMLKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQ
YPVSEITIAGNLAEMYQNIIAVGSDVETRSQIQTGSILIESMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=63.0872483221477, Blast_Score=585, Evalue=1e-168,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48383; Mature: 48383

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKAQMMNQKIELEAAVAKALEMATKHASAAEVAITKTTGISVSTLMGEVENVEFNRDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCEEECCCC
ALGITVYRGHRKGSASTSDLSDKAIQETVAAALDIANYTSEDPFSGPAPKELMAQNIPDL
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
DLFHPDDPEPDYAAQKAIEAEEAALAYHSSIKQSDGASYDSHYGIKVYGNSHGMLASYPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCC
SRHSISCSVIGVDKNGNMERDYSYTVARHRDDLWDAKKVGLDAAERTVSRLDAQKLNTMK
CCCEEEEEEEEECCCCCCCCCCEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
VPVMFSADVATGLIGHLVMAISGANLYRKSTFMLDKLNEKILPDWFNVSERPHILKGLAS
EEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCC
SPFDSEGLYTQDREIITDGVLATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFDQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
LKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLAEMYQNII
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEECCCHHHHHHHHH
AVGSDVETRSQIQTGSILIESMKIAGQ
HCCCCCHHHHHHHHHHHHEEHHHHCCC
>Mature Secondary Structure
MDAKAQMMNQKIELEAAVAKALEMATKHASAAEVAITKTTGISVSTLMGEVENVEFNRDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCEEECCCC
ALGITVYRGHRKGSASTSDLSDKAIQETVAAALDIANYTSEDPFSGPAPKELMAQNIPDL
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCC
DLFHPDDPEPDYAAQKAIEAEEAALAYHSSIKQSDGASYDSHYGIKVYGNSHGMLASYPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCC
SRHSISCSVIGVDKNGNMERDYSYTVARHRDDLWDAKKVGLDAAERTVSRLDAQKLNTMK
CCCEEEEEEEEECCCCCCCCCCEEHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEE
VPVMFSADVATGLIGHLVMAISGANLYRKSTFMLDKLNEKILPDWFNVSERPHILKGLAS
EEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCC
SPFDSEGLYTQDREIITDGVLATYLLTSYAARKLNMTPTGHAGGIHNWFVKSTGQSFDQM
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
LKTMGTGLLVTELMGQGVNTVTGDYSRGAAGFWVENGEIQYPVSEITIAGNLAEMYQNII
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEECCCHHHHHHHHH
AVGSDVETRSQIQTGSILIESMKIAGQ
HCCCCCHHHHHHHHHHHHEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]