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The map label for this gene is yafV [C]

Identifier: 209694084

GI number: 209694084

Start: 549469

End: 550233

Strand: Direct

Name: yafV [C]

Synonym: VSAL_I0489

Alternate gene names: 209694084

Gene position: 549469-550233 (Clockwise)

Preceding gene: 209694083

Following gene: 209694085

Centisome position: 16.52

GC content: 43.4

Gene sequence:

>765_bases
ATGAAGCAACTGAAACTCAATCTAAAAGGGTTACATCAACAAGGGGCTAAGCTTGCGCTAACACCTGAAAATACCCTTGT
CTTTGGTCAAAAAGAAGATTATGAAAAGCACGCAGAGCCGCTAGGGAAGGGGCCTCTGCAAGAGAAGTTGGCCAAATTAG
CAAAGCATTATCACTTATGGTTGATTATCGGATCATTTCCTATTCGAAATGCTGATGGATCGTTATCAAGCACTTGCCTT
GTCTTTAATCATGATGGTGATCTTGTTGAGCATTACCATAAATTGCATATGTTTGATGTCGATGTAGAAGATGGGCATCA
AAGCTACAGAGAATCCGATACTTTTACCGCGGGCAGTGAGATTAAAGTGGTTGATACGCCCATTGGAAAGATTGGTTTGT
CTATTTGCTATGACGTAAGGTTTCCACAACTTTACAGTGAATTACGCCAACAAGGTGCAGAAATACTGATTGTTCCTGCA
GCTTTCACAAAAGTTACAGGCTATGCGCATTGGGATATACTGCTACGTTCGCGAGCCATTGAGAACCAATGTTGGTTATT
GGCCGCTGGACAGTGGGGAAGTCATGGTTCAGGTCGAGAGACTTGGGGGCACTCTATGGTGGTTGACCCATGGGGCAATA
AAGTCGTCACTCAACGAGAGGGGACGGGGGTTATTGCTGCTGAAATAGATAAAAATCAAACGACTGCAATACGAAAAAAA
ATGCCCGTAGCACAACACGCAAGATTAGCTTGTAACATCCAATGA

Upstream 100 bases:

>100_bases
TAATGTAAATAGAGCCAAAAATAAGGACGTTAACGATGAGTAAAGTCGGTATTATTCAAATGAATTCAGGTGCGGATCCT
GAAGCAAATATGAAGCAAAT

Downstream 100 bases:

>100_bases
AGCTAATTGATTAAGAGTTTTATTGAGTAAAAGAGCGGTTTAACTATAAAACACGCTCTTTTTTATTCATAATTACTTTT
AGATGATAAATTCTAAAAAG

Product: carbon-nitrogen hydrolase

Products: beta-alanine; CO2; NH3

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKQLKLNLKGLHQQGAKLALTPENTLVFGQKEDYEKHAEPLGKGPLQEKLAKLAKHYHLWLIIGSFPIRNADGSLSSTCL
VFNHDGDLVEHYHKLHMFDVDVEDGHQSYRESDTFTAGSEIKVVDTPIGKIGLSICYDVRFPQLYSELRQQGAEILIVPA
AFTKVTGYAHWDILLRSRAIENQCWLLAAGQWGSHGSGRETWGHSMVVDPWGNKVVTQREGTGVIAAEIDKNQTTAIRKK
MPVAQHARLACNIQ

Sequences:

>Translated_254_residues
MKQLKLNLKGLHQQGAKLALTPENTLVFGQKEDYEKHAEPLGKGPLQEKLAKLAKHYHLWLIIGSFPIRNADGSLSSTCL
VFNHDGDLVEHYHKLHMFDVDVEDGHQSYRESDTFTAGSEIKVVDTPIGKIGLSICYDVRFPQLYSELRQQGAEILIVPA
AFTKVTGYAHWDILLRSRAIENQCWLLAAGQWGSHGSGRETWGHSMVVDPWGNKVVTQREGTGVIAAEIDKNQTTAIRKK
MPVAQHARLACNIQ
>Mature_254_residues
MKQLKLNLKGLHQQGAKLALTPENTLVFGQKEDYEKHAEPLGKGPLQEKLAKLAKHYHLWLIIGSFPIRNADGSLSSTCL
VFNHDGDLVEHYHKLHMFDVDVEDGHQSYRESDTFTAGSEIKVVDTPIGKIGLSICYDVRFPQLYSELRQQGAEILIVPA
AFTKVTGYAHWDILLRSRAIENQCWLLAAGQWGSHGSGRETWGHSMVVDPWGNKVVTQREGTGVIAAEIDKNQTTAIRKK
MPVAQHARLACNIQ

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=240, Percent_Identity=43.3333333333333, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI297632348, Length=240, Percent_Identity=43.3333333333333, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI297632350, Length=240, Percent_Identity=43.3333333333333, Blast_Score=186, Evalue=1e-47,
Organism=Homo sapiens, GI9910460, Length=236, Percent_Identity=37.7118644067797, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI297632346, Length=164, Percent_Identity=39.6341463414634, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1786412, Length=190, Percent_Identity=30, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17556280, Length=204, Percent_Identity=45.0980392156863, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6322335, Length=195, Percent_Identity=40.5128205128205, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323383, Length=241, Percent_Identity=34.0248962655602, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17933642, Length=237, Percent_Identity=40.084388185654, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21355835, Length=216, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.1.6

Molecular weight: Translated: 28388; Mature: 28388

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLKLNLKGLHQQGAKLALTPENTLVFGQKEDYEKHAEPLGKGPLQEKLAKLAKHYHLW
CCCEEEEHHHHCCCCCEEEECCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEE
LIIGSFPIRNADGSLSSTCLVFNHDGDLVEHYHKLHMFDVDVEDGHQSYRESDTFTAGSE
EEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCCE
IKVVDTPIGKIGLSICYDVRFPQLYSELRQQGAEILIVPAAFTKVTGYAHWDILLRSRAI
EEEEECCCCHHCEEEEEECCCHHHHHHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHCC
ENQCWLLAAGQWGSHGSGRETWGHSMVVDPWGNKVVTQREGTGVIAAEIDKNQTTAIRKK
CCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEECCCCHHHHHHH
MPVAQHARLACNIQ
CCHHHCCEEEEECC
>Mature Secondary Structure
MKQLKLNLKGLHQQGAKLALTPENTLVFGQKEDYEKHAEPLGKGPLQEKLAKLAKHYHLW
CCCEEEEHHHHCCCCCEEEECCCCEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEE
LIIGSFPIRNADGSLSSTCLVFNHDGDLVEHYHKLHMFDVDVEDGHQSYRESDTFTAGSE
EEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCCE
IKVVDTPIGKIGLSICYDVRFPQLYSELRQQGAEILIVPAAFTKVTGYAHWDILLRSRAI
EEEEECCCCHHCEEEEEECCCHHHHHHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHCC
ENQCWLLAAGQWGSHGSGRETWGHSMVVDPWGNKVVTQREGTGVIAAEIDKNQTTAIRKK
CCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEECCCCHHHHHHH
MPVAQHARLACNIQ
CCHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-carbamoyl-beta-alanine; H2O

Specific reaction: N-carbamoyl-beta-alanine + H2O = beta-alanine + CO2 + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]