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The map label for this gene is pucG [H]

Identifier: 209694044

GI number: 209694044

Start: 497687

End: 498796

Strand: Direct

Name: pucG [H]

Synonym: VSAL_I0445

Alternate gene names: 209694044

Gene position: 497687-498796 (Clockwise)

Preceding gene: 209694042

Following gene: 209694058

Centisome position: 14.97

GC content: 38.92

Gene sequence:

>1110_bases
ATGCAAAGTTTTATTCCACCACAAAGAACCCTTATGGGACCTGGTCCATCTGATGTTTCTCCCCAGGTATTACAAGCATT
GAGTCGCCCTACTATTGGTCATTTAGACCCATTGTTTATTAAAATGATGGATGAAGTAAAAGAGTTATTAAAATACGCAT
TTCAAACAAAAAATGAATTTACTATCGCGGTGTCAGCGCCAGGCAGTGCAGGAATGGAAACGTGTTTTGTTAATCTTGTA
GAGAGAGGCGAAAAAGTACTTGTTTGCCGTAATGGTGTTTTTGGTGAGCGAATGCGACAGAATGTAGAGCGTTGCGGTGG
AATTGCGATCGTTATTGATGATGAATGGGGAACTCCTGTATCGTTAGCAAAAGTAGAAGAAGCATTGATTAAAGATCCTG
AGATTAATGTTATCGCATTTGTGCACGCAGAGACATCAACAGGTGTATTAAGTGATGCGAAGTCTATCTCTGAATTGGCA
CAGAAATACAACTGTTTAACTATTGTAGACGCAGTAACATCGTTGGGTGGGGTTCCTTTATTCGTAGATGAATGGTTATT
AGATGCGGTTTATTCAGGATCTCAAAAATGCTTATCTTGTATACCTGGTCTTTCTCCGGTGACGTTTTCAAATAAAGCGG
TTGAGAAAATGAAGTCAAGAAAAGAACCGGTACAAAGTTGGTTTTTAGACCAAAGCCTAGTATTGGGTTACTGGAGTGGA
GAAGGCAAAAGAAGCTATCATCATACCGCACCAGTAAACAGTCTATATGCATTGCATGAATCATTATTAATGCTTAAAAA
TGAGGGGCTAGAGAATGCATGGAAGAGACATGATCATTTTCACCAAGAACTTAAGCGTGGACTAGAAAGTTTAGGTGTTT
CATTTATTGTTGATGAGAAATACCGATTACCACAGCTAAATGCGGTCTATATTCCTGATGGTACTGATGATGCTTTAGTT
CGAAAAGTATTATTAGAAGAATATAATTTAGAAATTGGCGCAGGATTAGGGGATTTAGCGGGTAAAGCTTGGCGCATTGG
ATTGATGGGGTATGCGGCACGTAAAGAAAACGTGTCTCTTTGTTTAAAAGCGTTATCTGACGTATTATAA

Upstream 100 bases:

>100_bases
TGTTGTTTTAGGCAGTTAAGTCACTCTAATTAGTTTGTCTAATTGAGTTTAATTATTTTTATGTTTATTATTTGAACACT
TACTATTCAAGGAGCGAGTC

Downstream 100 bases:

>100_bases
ATAACATGATGATAAAACGCCGTTGATAAATTCAACGGCGTTTTATCATTACTTTCTGATTTGACGTAACTTTTGGTCAA
GTTGAACGGTTTCGAAATTG

Product: aminotransferase class-V

Products: 3-hydroxypyruvate; L-alanine

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MQSFIPPQRTLMGPGPSDVSPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNEFTIAVSAPGSAGMETCFVNLV
ERGEKVLVCRNGVFGERMRQNVERCGGIAIVIDDEWGTPVSLAKVEEALIKDPEINVIAFVHAETSTGVLSDAKSISELA
QKYNCLTIVDAVTSLGGVPLFVDEWLLDAVYSGSQKCLSCIPGLSPVTFSNKAVEKMKSRKEPVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKNEGLENAWKRHDHFHQELKRGLESLGVSFIVDEKYRLPQLNAVYIPDGTDDALV
RKVLLEEYNLEIGAGLGDLAGKAWRIGLMGYAARKENVSLCLKALSDVL

Sequences:

>Translated_369_residues
MQSFIPPQRTLMGPGPSDVSPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNEFTIAVSAPGSAGMETCFVNLV
ERGEKVLVCRNGVFGERMRQNVERCGGIAIVIDDEWGTPVSLAKVEEALIKDPEINVIAFVHAETSTGVLSDAKSISELA
QKYNCLTIVDAVTSLGGVPLFVDEWLLDAVYSGSQKCLSCIPGLSPVTFSNKAVEKMKSRKEPVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKNEGLENAWKRHDHFHQELKRGLESLGVSFIVDEKYRLPQLNAVYIPDGTDDALV
RKVLLEEYNLEIGAGLGDLAGKAWRIGLMGYAARKENVSLCLKALSDVL
>Mature_369_residues
MQSFIPPQRTLMGPGPSDVSPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNEFTIAVSAPGSAGMETCFVNLV
ERGEKVLVCRNGVFGERMRQNVERCGGIAIVIDDEWGTPVSLAKVEEALIKDPEINVIAFVHAETSTGVLSDAKSISELA
QKYNCLTIVDAVTSLGGVPLFVDEWLLDAVYSGSQKCLSCIPGLSPVTFSNKAVEKMKSRKEPVQSWFLDQSLVLGYWSG
EGKRSYHHTAPVNSLYALHESLLMLKNEGLENAWKRHDHFHQELKRGLESLGVSFIVDEKYRLPQLNAVYIPDGTDDALV
RKVLLEEYNLEIGAGLGDLAGKAWRIGLMGYAARKENVSLCLKALSDVL

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=365, Percent_Identity=38.9041095890411, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17536281, Length=368, Percent_Identity=36.9565217391304, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321079, Length=377, Percent_Identity=29.1777188328912, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17530823, Length=367, Percent_Identity=37.874659400545, Blast_Score=257, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.51

Molecular weight: Translated: 40725; Mature: 40725

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSFIPPQRTLMGPGPSDVSPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNEF
CCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TIAVSAPGSAGMETCFVNLVERGEKVLVCRNGVFGERMRQNVERCGGIAIVIDDEWGTPV
EEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCH
SLAKVEEALIKDPEINVIAFVHAETSTGVLSDAKSISELAQKYNCLTIVDAVTSLGGVPL
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
FVDEWLLDAVYSGSQKCLSCIPGLSPVTFSNKAVEKMKSRKEPVQSWFLDQSLVLGYWSG
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECC
EGKRSYHHTAPVNSLYALHESLLMLKNEGLENAWKRHDHFHQELKRGLESLGVSFIVDEK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
YRLPQLNAVYIPDGTDDALVRKVLLEEYNLEIGAGLGDLAGKAWRIGLMGYAARKENVSL
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCEECCCCHHHCCCCEEEEHHHHHHHHHHHHH
CLKALSDVL
HHHHHHHCC
>Mature Secondary Structure
MQSFIPPQRTLMGPGPSDVSPQVLQALSRPTIGHLDPLFIKMMDEVKELLKYAFQTKNEF
CCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
TIAVSAPGSAGMETCFVNLVERGEKVLVCRNGVFGERMRQNVERCGGIAIVIDDEWGTPV
EEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCH
SLAKVEEALIKDPEINVIAFVHAETSTGVLSDAKSISELAQKYNCLTIVDAVTSLGGVPL
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
FVDEWLLDAVYSGSQKCLSCIPGLSPVTFSNKAVEKMKSRKEPVQSWFLDQSLVLGYWSG
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECC
EGKRSYHHTAPVNSLYALHESLLMLKNEGLENAWKRHDHFHQELKRGLESLGVSFIVDEK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
YRLPQLNAVYIPDGTDDALVRKVLLEEYNLEIGAGLGDLAGKAWRIGLMGYAARKENVSL
CCCCCCCEEECCCCCHHHHHHHHHHHHHCCEECCCCHHHCCCCEEEEHHHHHHHHHHHHH
CLKALSDVL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-serine; pyruvate

Specific reaction: L-serine + pyruvate = 3-hydroxypyruvate + L-alanine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]