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The map label for this gene is mdh

Identifier: 209693962

GI number: 209693962

Start: 394789

End: 395724

Strand: Direct

Name: mdh

Synonym: VSAL_I0359

Alternate gene names: 209693962

Gene position: 394789-395724 (Clockwise)

Preceding gene: 209693957

Following gene: 209693964

Centisome position: 11.87

GC content: 42.84

Gene sequence:

>936_bases
ATGAAAGTTGCTGTTATTGGCGCCGCTGGAGGCATCGGACAAGCATTAGCATTACTTCTTAAGAATCGATTACCAGCCGG
CTCGGATCTTGCTCTTTATGATATTGCACCGGTAACCCCTGGGGTTGCTGCGGATCTTAGTCATATCCCAACACCAGTTT
CAATTAAAGGGTATTGCGGAGAAGATCCAACACCAGCACTTGAAGGCGCTGATGTTGTTTTAATTTCTGCTGGTGTTGCT
CGTAAACCAGGAATGGATCGTTCAGATCTTTTCAATATTAATGCGGGTATTGTTAAGTCATTGGCTGAAAAAATTGCAGT
GACTTGTCCAAAGGCATGCATTGGTATTATTACTAACCCTGTTAATACAACCGTGGCAATTGCTGCTGAAGTATTAAAAA
AAGCAGGTGTATACGATAAAAATAAATTGTTTGGCGTAACCACACTGGATGTGATCCGTTCAGAGACGTTTGTGGCTGAG
TTAAAAGGTAAAAATCCAGGAGAGATCTGTGTTCCTGTTATTGGTGGGCATTCTGGTGTTACGATTCTTCCTCTGCTTTC
ACAAGTTGAGGGTGTTGAGTTCACCGCTGAAGAAGTGGCAGCATTAACACCGCGTATTCAAAACGCAGGAACGGAAGTTG
TGGAAGCTAAAGCTGGTGGCGGTTCTGCGACACTTTCAATGGGCCAAGCGGCATGTCGCTTTGGTTTATCACTAGTAAAA
GCGCTTAGTGGTGAGAAAGGGGTTGTAGAATGTGCGTATGTTGAAGGCAATGGTGAACACGCTCGTTTCTTCGCGCAACC
CATTCTTCTTGGTAAAAATGGTGTTGAAGAGATTCAACACTATGGCGAATTAAGTACTTTTGAACAAGATGCACTAGATA
GCATGCTAGATACGTTAAAAGCGGATATTAAAATTGGCGAAGAATTTATTAAGTAA

Upstream 100 bases:

>100_bases
ATAGCATTGAATTTCATTTGAGCTTGCTATAACGTCACTTTTAGTTTGAAAATAAATTAATAATAAAAAATATACCTCTA
CTACCTCAAGGAGAACGAAC

Downstream 100 bases:

>100_bases
ATTCTTAATGCATACGTAGATATCTTTAAATAGAAAAGGGCTGATACTTATTAAAAGTATCAGCCCTTTTATTTTATCTT
GATATAAAAGCCTTATTTGC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLAEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKGKNPGEICVPVIGGHSGVTILPLLSQVEGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEKGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQHYGELSTFEQDALDSMLDTLKADIKIGEEFIK

Sequences:

>Translated_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLAEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKGKNPGEICVPVIGGHSGVTILPLLSQVEGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEKGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQHYGELSTFEQDALDSMLDTLKADIKIGEEFIK
>Mature_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLAEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKGKNPGEICVPVIGGHSGVTILPLLSQVEGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEKGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQHYGELSTFEQDALDSMLDTLKADIKIGEEFIK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=57.3717948717949, Blast_Score=337, Evalue=7e-93,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=83.2258064516129, Blast_Score=523, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=56.2300319488818, Blast_Score=325, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=48.5714285714286, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=40.2366863905325, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6324446, Length=362, Percent_Identity=36.1878453038674, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=56.1290322580645, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=50, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=43.6666666666667, Blast_Score=241, Evalue=6e-64,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_ALISL (B6EL39)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261890.1
- ProteinModelPortal:   B6EL39
- GeneID:   6988256
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0359
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- ProtClustDB:   PRK05086
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01516
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11540:SF1
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01772

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 32162; Mature: 32162

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 177-177 BINDING 34-34 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 153-153 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVKSLAEKIAVTCPKACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAELKGKNPGEICVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCC
TILPLLSQVEGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
CHHHHHHHHCCCEECHHHHHHHCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGEKGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQHYGELSTFEQDALDSMLDTLK
HHCCCCCEEEEEEEECCCCEEEEECCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
ADIKIGEEFIK
HHHHHHHHHCC
>Mature Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVKSLAEKIAVTCPKACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAELKGKNPGEICVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCC
TILPLLSQVEGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
CHHHHHHHHCCCEECHHHHHHHCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGEKGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQHYGELSTFEQDALDSMLDTLK
HHCCCCCEEEEEEEECCCCEEEEECCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
ADIKIGEEFIK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA