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The map label for this gene is bsp6IR [H]

Identifier: 209554115

GI number: 209554115

Start: 704769

End: 705329

Strand: Reverse

Name: bsp6IR [H]

Synonym: UUR10_0588

Alternate gene names: 209554115

Gene position: 705329-704769 (Counterclockwise)

Preceding gene: 209554384

Following gene: 209554489

Centisome position: 80.66

GC content: 31.55

Gene sequence:

>561_bases
ATGAAACAAGAAACAATTAAATTAAAAGATGGTAGTATCGTTATTGGTGACACTTTTGATAAAAACGACTTTGAGATATT
AAGAGAAATTTTTAAAGAATGACTAATAATGAACAAAAAGTTAAAGAGTCTTGGTGGAAGAGGTTTGAATGTCCCTGATG
TGTTTAGTGAAGCTTTGTTTTGTTTAGCGTTTAATGCAGTAAGAACAAATGGTACAGCATATTCATATGATTGTGTTATA
AGAGACACCGGTGAAGGTGTTCAAGTAAAATCGGCTTCAATTCCAAATGATTGTACTTCTTTTGGCCCAACTTCAACTTG
AGACTTATTATACTTTGTTGACTTTGCTCCAAATGGTAAAGTTGATGGGTTAGTTAAATTTTATAAGATTGAAGATGATG
ATGTTTACAATTTAGTTTTAAACGCTAAGAAAGGCGAAACATTCAAAGATCAACAAGCACAAGGAAGAAGGCCTAGATTT
TCCATACAATCAGCAATTATTAGATCAAAGAGGCTATTACCTATTAAAACCATAAATATAATAACTGGCGAAGGTCTATA
A

Upstream 100 bases:

>100_bases
ATTGATCACAAGATGTTAAAAAACCTAATTAAATAAAAAGCACAAATGTTTTATCATTATTTTGAATATTGAAAAAAAGT
AAAACATTGAGGATAATAGC

Downstream 100 bases:

>100_bases
ATTAAGGTTATATTAAATACTACTTCTTATATTATTCTTCGTCAGCTTTCAATGCTTCCAATAGTTTTTCAACTACTCGT
CTTACGACAGGAACTGATAC

Product: type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.Bsp6I; Endonuclease Bsp6I; Type II restriction enzyme Bsp6I [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKQETIKLKDGSIVIGDTFDKNDFEILREIFKEWLIMNKKLKSLGGRGLNVPDVFSEALFCLAFNAVRTNGTAYSYDCVI
RDTGEGVQVKSASIPNDCTSFGPTSTWDLLYFVDFAPNGKVDGLVKFYKIEDDDVYNLVLNAKKGETFKDQQAQGRRPRF
SIQSAIIRSKRLLPIKTINIITGEGL

Sequences:

>Translated_186_residues
MKQETIKLKDGSIVIGDTFDKNDFEILREIFKE*LIMNKKLKSLGGRGLNVPDVFSEALFCLAFNAVRTNGTAYSYDCVI
RDTGEGVQVKSASIPNDCTSFGPTST*DLLYFVDFAPNGKVDGLVKFYKIEDDDVYNLVLNAKKGETFKDQQAQGRRPRF
SIQSAIIRSKRLLPIKTINIITGEGL
>Mature_186_residues
MKQETIKLKDGSIVIGDTFDKNDFEILREIFKE*LIMNKKLKSLGGRGLNVPDVFSEALFCLAFNAVRTNGTAYSYDCVI
RDTGEGVQVKSASIPNDCTSFGPTST*DLLYFVDFAPNGKVDGLVKFYKIEDDDVYNLVLNAKKGETFKDQQAQGRRPRF
SIQSAIIRSKRLLPIKTINIITGEGL

Specific function: Recognizes the double-stranded sequence GCNGC and cleaves after C-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019037 [H]

Pfam domain/function: PF09504 RE_Bsp6I [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 20478; Mature: 20478

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQETIKLKDGSIVIGDTFDKNDFEILREIFKELIMNKKLKSLGGRGLNVPDVFSEALFC
CCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LAFNAVRTNGTAYSYDCVIRDTGEGVQVKSASIPNDCTSFGPTSTDLLYFVDFAPNGKVD
HHHHHHCCCCCEEEEEEEEEECCCCEEEECCCCCCCHHCCCCCCCCEEEEEEECCCCCCC
GLVKFYKIEDDDVYNLVLNAKKGETFKDQQAQGRRPRFSIQSAIIRSKRLLPIKTINIIT
CEEEEEEECCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEEE
GEGL
CCCC
>Mature Secondary Structure
MKQETIKLKDGSIVIGDTFDKNDFEILREIFKELIMNKKLKSLGGRGLNVPDVFSEALFC
CCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LAFNAVRTNGTAYSYDCVIRDTGEGVQVKSASIPNDCTSFGPTSTDLLYFVDFAPNGKVD
HHHHHHCCCCCEEEEEEEEEECCCCEEEECCCCCCCHHCCCCCCCCEEEEEEECCCCCCC
GLVKFYKIEDDDVYNLVLNAKKGETFKDQQAQGRRPRFSIQSAIIRSKRLLPIKTINIIT
CEEEEEEECCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCEEEEEEEEE
GEGL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7607501 [H]