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The map label for this gene is pcrA [H]

Identifier: 209554036

GI number: 209554036

Start: 696136

End: 698367

Strand: Reverse

Name: pcrA [H]

Synonym: UUR10_0577

Alternate gene names: 209554036

Gene position: 698367-696136 (Counterclockwise)

Preceding gene: 209554073

Following gene: 209553989

Centisome position: 79.86

GC content: 26.88

Gene sequence:

>2232_bases
ATGGGAGTAGGAATTGATTATTCACGCTTAAATCAAGAACAAAAAGAAGCAGTTACAGCAGATTTAGGACCACAATTAGT
AATTGCAGGGGCAGGAACAGGAAAAACATCTGTTTTAACTTTACGTATTGCTCATTTAATTACTGAAAAACACATTCATC
CATCACGAATTTTAGGTTTTACTTTTACTAACAAAGCTGCTGATGAAATGAAAGAGCGTGTTGGAAAAACAATCGGTGTT
TCTATTCCTTATTTAAGTACTTTCCATTCAATGTGTGTAAAGATTTTACAACAAGATATTCATTATTTAAATTATCATAA
TAATATTAAAATCATTGATGCAGATGATCAAGAAGTTTTACTTAAAGAAATTTTTGATCAATTAAGTATCGAAAAAAAAT
CGCCAATCATTAAAAAAACGATTAAAATGATCAGTAAAGTTAAAAATAAATTTTTTGATCAAAGCGACATCATGAATGAA
AAAAATCGTAAGTATTTAGAAATAGTTGATAGAAATGATGCGCAAAAATTAGCTGATATTTACACAATTTATTGTGATCG
TTGTTTTAAATTAAATTATGTTGATTTTGACGATTTAATTAATTTAACTTATAAATTATTTGCTGAGTTTCCAGAAGTTT
TAGAAAAATGACAAAATAAATTTGATTATATTCTTGTTGATGAATTTCAAGATACTAATAAAATTCAATATGATTTAATT
AGTTTATTAGCAGCTAAACACCAAAATTTATTTGTGGTTGGTGATCCAGATCAAATGATTTATTCATTTAGAGGCGCTGA
ACAATGAATCATTAATAATTTTAATCAAAATTTTAAAAATACAAAAGTAACAATTTTAAAAACAAATTATCGATCAACTC
AACCAATTTTAGACACAGCCAATCGATTAATCGATGCTAATAATAACAGTTACAAAAAGAATTTAACTGCTTTTAACACT
AATGATCATAACATACCAATTTATTTACGAGGTCAGAATCAAATTGATGAAGCTAATTGAATTGCTAGAACTGTTAAAGA
ATTATTAGAAGAAGGTACACCAGCTAACCAAATTGCTATTTTGTTTCGAAGCAATCACTATTCTCGTACAATTGAACAAT
CAATGATGCGCGAGAGTATTCCTTATACAATTCTTGGTAGTAAAAAGTTTTATGAACGTGCTGAAATCAAGGACATGATT
GCTTATTTAAAAGTAGTTAATGATTTAGATGAACTATCTTTTTTAAGAATTATTAATACACCACGTCGTGCGATTGGTCC
AACTACGTTTGAACACGTTAAACATTATGCCATAAACAATAATTTATTTTTATTTGAAGCATTAGCTGAGGTTGAAAAAA
ACCACTTAATAAATAACACACAAAAAAAGAATATTTTAAATTTTGTTAATTTAATTAAAGAAATTCGTGATGAAATGGAA
GATTTAAAACTTCATGAAGTTTTAGAATTAATTTATAAAAAAGTTAACTATGAAGCTTATTTATTAGAAAATGAAAGAGC
AGAAGATAAAATTGATAATGTTTCTGAATTAAAACGTGCTATGAAGATGTATGTTGATCGTCACCCAGATGATACAATTA
ATGATTATTTAAATTCAATTGCCTTATATTTAAATAAGGATAGCAAAGATAGTAAAGAAAATGTTTTATTAATGACAGTT
CATAATTCTAAGGGTTTAGAATACGAAAACGTTTTTGTTGTTGGTATGAATGAAGGTCATCTTCCCTCTGATCGAGCAAT
TGATGATGATCCAATTAAAGGGGTTGAAGAAGAGCGAAGAATTGCTTATGTAGCACTAACACGAGCGAAAAAAAATCTTT
ATATTTCATCTGCTTGTTGTTATGATCCATTAGCAAGAAGACAAGTATTTGAAAGTCGTTTTATTAATGAAATTGGTTTT
AATAATTTAAAAATTATAAATAGTAGTTTTAAAAATAATAAACCAGAGGATATGCCATTAAAATCGTTTTTAAAACAACA
AGAAGAACGTTCATGATTTGATTCACAACAAAAACAAAAAGAAGCTGAAGATAATTTTTATCAAACAATGAAAAACGATT
TTGAAATTGGCGAAAGAATTGTGCACACAAGTTTTGGTAATGGTGTTATTATTGGAATTGATGGTGATATTTTAACAATT
GCTTTTAGCAAATATCGTCAACCAAAACAAATTAGTTTTAATCATAAAACAATCGTAAGAAGAATGGGTTAA

Upstream 100 bases:

>100_bases
ACCAAAACATAAAACAAAACGTGAAGTTGAGATTCAAAAAATCGATAACTTAATTCAAGAACTAGATAAAATATAAAATG
AAAAACAAGGAGAAGTTTTT

Downstream 100 bases:

>100_bases
AAAATGAAAATTTTAGCTATTGGAGATATTTTTTCAAAACAAGGAAGAAAAGCAGTGGCCAATGAATTGCCTAAGCTAAA
ACAAGAGCATCAAATTGATT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MGVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGFTFTNKAADEMKERVGKTIGV
SIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVLLKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNE
KNRKYLEIVDRNDAQKLADIYTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEKWQNKFDYILVDEFQDTNKIQYDLI
SLLAAKHQNLFVVGDPDQMIYSFRGAEQWIINNFNQNFKNTKVTILKTNYRSTQPILDTANRLIDANNNSYKKNLTAFNT
NDHNIPIYLRGQNQIDEANWIARTVKELLEEGTPANQIAILFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMI
AYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEME
DLKLHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALYLNKDSKDSKENVLLMTV
HNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGF
NNLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTI
AFSKYRQPKQISFNHKTIVRRMG

Sequences:

>Translated_743_residues
MGVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGFTFTNKAADEMKERVGKTIGV
SIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVLLKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNE
KNRKYLEIVDRNDAQKLADIYTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEK*QNKFDYILVDEFQDTNKIQYDLI
SLLAAKHQNLFVVGDPDQMIYSFRGAEQ*IINNFNQNFKNTKVTILKTNYRSTQPILDTANRLIDANNNSYKKNLTAFNT
NDHNIPIYLRGQNQIDEAN*IARTVKELLEEGTPANQIAILFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMI
AYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEME
DLKLHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALYLNKDSKDSKENVLLMTV
HNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGF
NNLKIINSSFKNNKPEDMPLKSFLKQQEERS*FDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTI
AFSKYRQPKQISFNHKTIVRRMG
>Mature_742_residues
GVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGFTFTNKAADEMKERVGKTIGVS
IPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVLLKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNEK
NRKYLEIVDRNDAQKLADIYTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEK*QNKFDYILVDEFQDTNKIQYDLIS
LLAAKHQNLFVVGDPDQMIYSFRGAEQ*IINNFNQNFKNTKVTILKTNYRSTQPILDTANRLIDANNNSYKKNLTAFNTN
DHNIPIYLRGQNQIDEAN*IARTVKELLEEGTPANQIAILFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIA
YLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMED
LKLHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALYLNKDSKDSKENVLLMTVH
NSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFN
NLKIINSSFKNNKPEDMPLKSFLKQQEERS*FDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTIA
FSKYRQPKQISFNHKTIVRRMG

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=729, Percent_Identity=32.3731138545953, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI48994965, Length=658, Percent_Identity=36.7781155015198, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI1787196, Length=412, Percent_Identity=27.6699029126214, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6322369, Length=752, Percent_Identity=29.7872340425532, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6324477, Length=659, Percent_Identity=22.3065250379363, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85741; Mature: 85610

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGF
CCCCCCHHHCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEE
TFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVL
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHH
LKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNEKNRKYLEIVDRNDAQKLADI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEECCCCHHHHHHH
YTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEKQNKFDYILVDEFQDTNKIQYDLIS
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
LLAAKHQNLFVVGDPDQMIYSFRGAEQIINNFNQNFKNTKVTILKTNYRSTQPILDTANR
HHHHCCCCEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
LIDANNNSYKKNLTAFNTNDHNIPIYLRGQNQIDEANIARTVKELLEEGTPANQIAILFR
HEECCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
SNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRR
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLK
HCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALY
HHHHHHHHHHHCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
LNKDSKDSKENVLLMTVHNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAY
ECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEE
VALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSF
EEEEHHHCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCHHHH
LKQQEERSFDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTIAFSK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEEHHH
YRQPKQISFNHKTIVRRMG
CCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
GVGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAHLITEKHIHPSRILGF
CCCCCHHHCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEE
TFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDADDQEVL
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHH
LKEIFDQLSIEKKSPIIKKTIKMISKVKNKFFDQSDIMNEKNRKYLEIVDRNDAQKLADI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEECCCCHHHHHHH
YTIYCDRCFKLNYVDFDDLINLTYKLFAEFPEVLEKQNKFDYILVDEFQDTNKIQYDLIS
HHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
LLAAKHQNLFVVGDPDQMIYSFRGAEQIINNFNQNFKNTKVTILKTNYRSTQPILDTANR
HHHHCCCCEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHH
LIDANNNSYKKNLTAFNTNDHNIPIYLRGQNQIDEANIARTVKELLEEGTPANQIAILFR
HEECCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
SNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRR
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLK
HCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LHEVLELIYKKVNYEAYLLENERAEDKIDNVSELKRAMKMYVDRHPDDTINDYLNSIALY
HHHHHHHHHHHCCCEEEEECCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
LNKDSKDSKENVLLMTVHNSKGLEYENVFVVGMNEGHLPSDRAIDDDPIKGVEEERRIAY
ECCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEE
VALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSF
EEEEHHHCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCHHHH
LKQQEERSFDSQQKQKEAEDNFYQTMKNDFEIGERIVHTSFGNGVIIGIDGDILTIAFSK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEEHHH
YRQPKQISFNHKTIVRRMG
CCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]