Click here to switch to the map view.

The map label for this gene is 209552130

Identifier: 209552130

GI number: 209552130

Start: 233859

End: 235289

Strand: Reverse

Name: 209552130

Synonym: Rleg2_6284

Alternate gene names: NA

Gene position: 235289-233859 (Counterclockwise)

Preceding gene: 209552131

Following gene: 209552126

Centisome position: 76.21

GC content: 56.11

Gene sequence:

>1431_bases
ATGAATTCAACAGAATACAGAAGCCGGGATGCAATCGATCTTGCTCGGATGCTCCATAATAAGGAGCTGACGCCGCTCGA
ACTCATGGATATCGCGATCGAGCGCGCCGAAAGTGTGAACGTCAAGATCAACGCGCTATGCAACGTGAATTTCGAGCATG
GTCGGGAAATCGCTCGGTCTGCCTCATTAAAGGGGCGTTTCGGAGCTCTCCCTTTCCTGTTGAAGGATTCCGGTCTTGGA
TATCCGCCCCTTTCTAACTCACTTGGCTCCCGGTTGTTCGCCGGTTTGAAAATCGACTTCAACTCGACGCTGACCGAGAG
ATTCTTGGACGACGGGATGCTACCGTTCGGCCGGACGACCGTGCCGGAATTCAGCATGGCTCCGACCACCGAGGCGGTCC
AGAATGGTGGACCTACCCGCAACCCCTGGGACCAATCGCGCTCTCCTGGGGGCTCGAGTGGCGGAGCTGCCGCGGCTGTT
GCTTCCGGAATTATCCCCTTGGCCCATGGCAGCGACGGAGGCGGATCGATACGAATTCCAGCGTCCTGTTGCGGCATCTA
TGGCTTGAAGCCTTCGAGGGGGCTCATTCCTGCGGGTCCGGCGAGAGGCGAGGGGTGGGGAGGCCTGGCGACGGACGGCG
TGTTGTCGCGATCGGTTCGCGACACTGCCGCCGCGCTCGATGGAGTTGCGGGGATGGAGTTAGGCGCTCCATACGCCGCC
CCCAAAAAATCAGGAGCATATACGTTTGTACTGAATAGGCCTTTCGAGAAGCCCAAACGAATAGCCATGTGGAGTATTGG
GTTTGATGACATTCCGATCGACCCGGAATGCCATCTCGCGCTACAAACTGCCAAAGGCCTGCTGGAGAAAATGGGCCACG
AAGTCGTAGATGCCCCCCTTCCTCCTATCCAATTTGCAAAGTTCGTCGAGGCCCAGATCAACGTGATGGCCGCGAACGTT
ACAGTTGCTGTCAACGGTAAGGTCCGAAACGATCTTGCGTCCGGGTGGGAGCAGAAGCTTGAGCCGGCCATTCTCGACGC
CTACCAGAGAGGCAGGGCCCTTAATGCAGAAACCTACGCGCTTGCGATCACGCGTTTCCATACGATCGGGCGGCAATTGG
AAGTCTATATACGTGATTATGACTTCATTCTCACGCCAACGCTTATGCAGCCGCCTGTCGGGCTCGGCTTCATCACCACG
AACTGTGATTTCGCTTCGTTCCGTACCAAAATCAGTAGATATGCCACGTTCCTTGCAATCATAAATGCCTCTGGACAACC
TGCGGCAAGTATTCCTTTGCATTGGTCCAAGGATGGACTTCCGATCGGCGTTCAGATGGTTGCTCCGTTTGGAGGCGAGG
CCGATTTGCTCCGGATGTCGGCTCGTTTAGAAGAAGCACAGCCTTGGTTCAACAGGACGCCGGAAATCTAA

Upstream 100 bases:

>100_bases
TCCCCAAGGGAAGACTGGAAAGGTTGACAAGAAGGCATTGAAAATGCGCGCGGAAGAACAAGCGGCTGCAGTTTGACGCG
CGAACAAGAGAGATACGCTG

Downstream 100 bases:

>100_bases
TGTGGGGCACCATTCGTCGATATTACAAGATTTACTCCGAGTTGCTGGACCCGGATCCTCGCGAGTATGAGAGGTTGTTC
ACAGCCGCGATTGGTGGCGG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNSTEYRSRDAIDLARMLHNKELTPLELMDIAIERAESVNVKINALCNVNFEHGREIARSASLKGRFGALPFLLKDSGLG
YPPLSNSLGSRLFAGLKIDFNSTLTERFLDDGMLPFGRTTVPEFSMAPTTEAVQNGGPTRNPWDQSRSPGGSSGGAAAAV
ASGIIPLAHGSDGGGSIRIPASCCGIYGLKPSRGLIPAGPARGEGWGGLATDGVLSRSVRDTAAALDGVAGMELGAPYAA
PKKSGAYTFVLNRPFEKPKRIAMWSIGFDDIPIDPECHLALQTAKGLLEKMGHEVVDAPLPPIQFAKFVEAQINVMAANV
TVAVNGKVRNDLASGWEQKLEPAILDAYQRGRALNAETYALAITRFHTIGRQLEVYIRDYDFILTPTLMQPPVGLGFITT
NCDFASFRTKISRYATFLAIINASGQPAASIPLHWSKDGLPIGVQMVAPFGGEADLLRMSARLEEAQPWFNRTPEI

Sequences:

>Translated_476_residues
MNSTEYRSRDAIDLARMLHNKELTPLELMDIAIERAESVNVKINALCNVNFEHGREIARSASLKGRFGALPFLLKDSGLG
YPPLSNSLGSRLFAGLKIDFNSTLTERFLDDGMLPFGRTTVPEFSMAPTTEAVQNGGPTRNPWDQSRSPGGSSGGAAAAV
ASGIIPLAHGSDGGGSIRIPASCCGIYGLKPSRGLIPAGPARGEGWGGLATDGVLSRSVRDTAAALDGVAGMELGAPYAA
PKKSGAYTFVLNRPFEKPKRIAMWSIGFDDIPIDPECHLALQTAKGLLEKMGHEVVDAPLPPIQFAKFVEAQINVMAANV
TVAVNGKVRNDLASGWEQKLEPAILDAYQRGRALNAETYALAITRFHTIGRQLEVYIRDYDFILTPTLMQPPVGLGFITT
NCDFASFRTKISRYATFLAIINASGQPAASIPLHWSKDGLPIGVQMVAPFGGEADLLRMSARLEEAQPWFNRTPEI
>Mature_476_residues
MNSTEYRSRDAIDLARMLHNKELTPLELMDIAIERAESVNVKINALCNVNFEHGREIARSASLKGRFGALPFLLKDSGLG
YPPLSNSLGSRLFAGLKIDFNSTLTERFLDDGMLPFGRTTVPEFSMAPTTEAVQNGGPTRNPWDQSRSPGGSSGGAAAAV
ASGIIPLAHGSDGGGSIRIPASCCGIYGLKPSRGLIPAGPARGEGWGGLATDGVLSRSVRDTAAALDGVAGMELGAPYAA
PKKSGAYTFVLNRPFEKPKRIAMWSIGFDDIPIDPECHLALQTAKGLLEKMGHEVVDAPLPPIQFAKFVEAQINVMAANV
TVAVNGKVRNDLASGWEQKLEPAILDAYQRGRALNAETYALAITRFHTIGRQLEVYIRDYDFILTPTLMQPPVGLGFITT
NCDFASFRTKISRYATFLAIINASGQPAASIPLHWSKDGLPIGVQMVAPFGGEADLLRMSARLEEAQPWFNRTPEI

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=519, Percent_Identity=24.6628131021195, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI195972892, Length=201, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=445, Percent_Identity=26.5168539325843, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=322, Percent_Identity=26.7080745341615, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=336, Percent_Identity=29.4642857142857, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=336, Percent_Identity=29.4642857142857, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=331, Percent_Identity=28.3987915407855, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=204, Percent_Identity=33.8235294117647, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=211, Percent_Identity=28.9099526066351, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32565337, Length=221, Percent_Identity=29.8642533936652, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=23.921568627451, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=24.65483234714, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648113, Length=446, Percent_Identity=30.0448430493274, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356731, Length=486, Percent_Identity=23.8683127572016, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550774, Length=233, Percent_Identity=30.9012875536481, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648435, Length=233, Percent_Identity=30.9012875536481, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648437, Length=233, Percent_Identity=30.9012875536481, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=25.0996015936255, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=25.0996015936255, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=25.0996015936255, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=25.0996015936255, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 51153; Mature: 51153

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTEYRSRDAIDLARMLHNKELTPLELMDIAIERAESVNVKINALCNVNFEHGREIARS
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHH
ASLKGRFGALPFLLKDSGLGYPPLSNSLGSRLFAGLKIDFNSTLTERFLDDGMLPFGRTT
HCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEEECCHHHHHHHHHCCCCCCCCCC
VPEFSMAPTTEAVQNGGPTRNPWDQSRSPGGSSGGAAAAVASGIIPLAHGSDGGGSIRIP
CCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCCCEEEEC
ASCCGIYGLKPSRGLIPAGPARGEGWGGLATDGVLSRSVRDTAAALDGVAGMELGAPYAA
HHHHCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PKKSGAYTFVLNRPFEKPKRIAMWSIGFDDIPIDPECHLALQTAKGLLEKMGHEVVDAPL
CCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCC
PPIQFAKFVEAQINVMAANVTVAVNGKVRNDLASGWEQKLEPAILDAYQRGRALNAETYA
CHHHHHHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEE
LAITRFHTIGRQLEVYIRDYDFILTPTLMQPPVGLGFITTNCDFASFRTKISRYATFLAI
EEHHHHHHHCCEEEEEEECCCEEEECHHHCCCCCEEEEECCCCHHHHHHHHHHHHEEEEE
INASGQPAASIPLHWSKDGLPIGVQMVAPFGGEADLLRMSARLEEAQPWFNRTPEI
EECCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MNSTEYRSRDAIDLARMLHNKELTPLELMDIAIERAESVNVKINALCNVNFEHGREIARS
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHH
ASLKGRFGALPFLLKDSGLGYPPLSNSLGSRLFAGLKIDFNSTLTERFLDDGMLPFGRTT
HCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEEECCHHHHHHHHHCCCCCCCCCC
VPEFSMAPTTEAVQNGGPTRNPWDQSRSPGGSSGGAAAAVASGIIPLAHGSDGGGSIRIP
CCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEECCCCCCCEEEEC
ASCCGIYGLKPSRGLIPAGPARGEGWGGLATDGVLSRSVRDTAAALDGVAGMELGAPYAA
HHHHCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PKKSGAYTFVLNRPFEKPKRIAMWSIGFDDIPIDPECHLALQTAKGLLEKMGHEVVDAPL
CCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCC
PPIQFAKFVEAQINVMAANVTVAVNGKVRNDLASGWEQKLEPAILDAYQRGRALNAETYA
CHHHHHHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEE
LAITRFHTIGRQLEVYIRDYDFILTPTLMQPPVGLGFITTNCDFASFRTKISRYATFLAI
EEHHHHHHHCCEEEEEEECCCEEEECHHHCCCCCEEEEECCCCHHHHHHHHHHHHEEEEE
INASGQPAASIPLHWSKDGLPIGVQMVAPFGGEADLLRMSARLEEAQPWFNRTPEI
EECCCCCCCCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]