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The map label for this gene is ampR [H]

Identifier: 209552003

GI number: 209552003

Start: 80407

End: 81330

Strand: Reverse

Name: ampR [H]

Synonym: Rleg2_6145

Alternate gene names: 209552003

Gene position: 81330-80407 (Counterclockwise)

Preceding gene: 209552010

Following gene: 209552002

Centisome position: 26.34

GC content: 55.19

Gene sequence:

>924_bases
ATGGGAGAGCTTCGTCGAGACGTTCATTTGCTGAGGAATCTCGTGACCTTTGAGGCGGTTTGCCGTTCTCAAAATTTCAC
AAATGCCGCCGCGGACTTGGGGATTACGCGCGTCGCCGTAAGCCGCCAGATCGCCGACCTTGAAGACGGTATTCAGCAGA
AGCTTTTCAGCAGAATGCACCGCAAAGTCGTTCTCACCGCAGCAGGGGAGGAATTCGCCAACACCGTCAATCCAGCACTC
GATGAGATTTCCCACGCGTTGAACAAATACCGGCACGACGCGGGCGGATCCAGGCTTTCGGTAACGATTACAACGGCGTT
TGCGACATTTTGGCTCATGCCCCGGCTCGTCGACCTGGGTTCGCGATTCCCTGATCTTGAGGTAAACCTCGTTGTTTCCG
ACCGCTATCTCGATCTGGCAGCGGAAAACGTGGATATCGCTATCCGCTACATGCCCGCGCCACCAGAAGGCGACGACTGG
GTGCGCCTCATGCGAGAAGAAATATTCCCGGTCTACAGCCCTAAGTACCCCATCCGAACCACGTTAACCGCGCCAGTCGA
TCTTCTATCGGAGAGGCTTCTCTTTCTCTCGGGTCAATATCGTCCTGAGGCCAGGTGGGGACACTGGTTTAAAGTCCACA
ACTTGCCTCAGCCCGACGAAAAAAACGGGGTCACGGTCAACACCTACATCAACATGGTGCAGGCAGCGTTGGAGGGGCAG
GGCATTGCTCTGGCAGGCTTTCCGCTAATGAATGGCTACTTGCAGGACGGAACTTTAGTGAGGATGACCAGCATCGAGCC
GTTAATGAGGGAATATTACTACGTCCTCAACAGGTCTCCCCGGAAGGCTGTGACACGGCTTTTCTACGATTGGCTGTTCG
AACAGGCCGCGTCGGAAGGCTACGGCAACAGTGTCTTTCGGTAG

Upstream 100 bases:

>100_bases
CATCAGAACGTTCCCACTTGCTGTTATTTTCAGCAGGTTGGCAGCAAGTCACGGCGTTGTAAAGTTGACTAATAGTTCCA
CTTTTGTTAACTAGGAGTAT

Downstream 100 bases:

>100_bases
GTGCGGTCTAGTATAGCCGAATTTCTCCATGGGATCATCTGGGTTACTTATCAATGACTGAGGCCGAACCAACACAACCA
GCCATCCACCGATATCCAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MGELRRDVHLLRNLVTFEAVCRSQNFTNAAADLGITRVAVSRQIADLEDGIQQKLFSRMHRKVVLTAAGEEFANTVNPAL
DEISHALNKYRHDAGGSRLSVTITTAFATFWLMPRLVDLGSRFPDLEVNLVVSDRYLDLAAENVDIAIRYMPAPPEGDDW
VRLMREEIFPVYSPKYPIRTTLTAPVDLLSERLLFLSGQYRPEARWGHWFKVHNLPQPDEKNGVTVNTYINMVQAALEGQ
GIALAGFPLMNGYLQDGTLVRMTSIEPLMREYYYVLNRSPRKAVTRLFYDWLFEQAASEGYGNSVFR

Sequences:

>Translated_307_residues
MGELRRDVHLLRNLVTFEAVCRSQNFTNAAADLGITRVAVSRQIADLEDGIQQKLFSRMHRKVVLTAAGEEFANTVNPAL
DEISHALNKYRHDAGGSRLSVTITTAFATFWLMPRLVDLGSRFPDLEVNLVVSDRYLDLAAENVDIAIRYMPAPPEGDDW
VRLMREEIFPVYSPKYPIRTTLTAPVDLLSERLLFLSGQYRPEARWGHWFKVHNLPQPDEKNGVTVNTYINMVQAALEGQ
GIALAGFPLMNGYLQDGTLVRMTSIEPLMREYYYVLNRSPRKAVTRLFYDWLFEQAASEGYGNSVFR
>Mature_306_residues
GELRRDVHLLRNLVTFEAVCRSQNFTNAAADLGITRVAVSRQIADLEDGIQQKLFSRMHRKVVLTAAGEEFANTVNPALD
EISHALNKYRHDAGGSRLSVTITTAFATFWLMPRLVDLGSRFPDLEVNLVVSDRYLDLAAENVDIAIRYMPAPPEGDDWV
RLMREEIFPVYSPKYPIRTTLTAPVDLLSERLLFLSGQYRPEARWGHWFKVHNLPQPDEKNGVTVNTYINMVQAALEGQG
IALAGFPLMNGYLQDGTLVRMTSIEPLMREYYYVLNRSPRKAVTRLFYDWLFEQAASEGYGNSVFR

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=28.7162162162162, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=30, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786448, Length=247, Percent_Identity=27.5303643724696, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=27.3333333333333, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=26.4026402640264, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790262, Length=283, Percent_Identity=27.208480565371, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=262, Percent_Identity=24.0458015267176, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34920; Mature: 34789

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGELRRDVHLLRNLVTFEAVCRSQNFTNAAADLGITRVAVSRQIADLEDGIQQKLFSRMH
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKVVLTAAGEEFANTVNPALDEISHALNKYRHDAGGSRLSVTITTAFATFWLMPRLVDLG
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHC
SRFPDLEVNLVVSDRYLDLAAENVDIAIRYMPAPPEGDDWVRLMREEIFPVYSPKYPIRT
CCCCCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEE
TLTAPVDLLSERLLFLSGQYRPEARWGHWFKVHNLPQPDEKNGVTVNTYINMVQAALEGQ
ECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCC
GIALAGFPLMNGYLQDGTLVRMTSIEPLMREYYYVLNRSPRKAVTRLFYDWLFEQAASEG
CEEEECCHHHCCEECCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
YGNSVFR
CCCCCCC
>Mature Secondary Structure 
GELRRDVHLLRNLVTFEAVCRSQNFTNAAADLGITRVAVSRQIADLEDGIQQKLFSRMH
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKVVLTAAGEEFANTVNPALDEISHALNKYRHDAGGSRLSVTITTAFATFWLMPRLVDLG
HEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHC
SRFPDLEVNLVVSDRYLDLAAENVDIAIRYMPAPPEGDDWVRLMREEIFPVYSPKYPIRT
CCCCCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEE
TLTAPVDLLSERLLFLSGQYRPEARWGHWFKVHNLPQPDEKNGVTVNTYINMVQAALEGQ
ECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCC
GIALAGFPLMNGYLQDGTLVRMTSIEPLMREYYYVLNRSPRKAVTRLFYDWLFEQAASEG
CEEEECCHHHCCEECCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
YGNSVFR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]