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The map label for this gene is proP [H]

Identifier: 209551992

GI number: 209551992

Start: 66767

End: 68425

Strand: Reverse

Name: proP [H]

Synonym: Rleg2_6133

Alternate gene names: 209551992

Gene position: 68425-66767 (Counterclockwise)

Preceding gene: 209551993

Following gene: 209551991

Centisome position: 22.16

GC content: 58.77

Gene sequence:

>1659_bases
ATGACACATGTCGAAACCATGACTCAGGCTCAGGGGATATCCCGCAGAGACAGGAAGGTTATCCTTGCAGCTTCTCTGGG
GACGGTTTTTGAGTTTTACGACTTCTTTCTAATCGGACTTGTCGCCACCGAAATCGCCAAGGCGTTTTTCTCGGGCGTCA
ATCCGACAGCGGGCTTCATCTTCACCCTCTTGGGTTTCGCCGCTGGCTTCATGCTGAGGCCATTCGGCGCGATTGTGTTC
GGACGTCTCGGCGACCTGGTGGGCCGGAAGTACACGTTCCTCGTCACGATCGTTCTCATGGGCGGCTCGACGTTCCTGAT
CGGGCTTCTGCCGGCTTACGCGACGATCGGGGTGGCGGCGCCAATCGCATTCGTCGCCATGAGAATGCTTCAGGGCCTGG
CGCTCGGAGGCGAGTTCGGGGGCGCCATGGTGTACGTGGCGGAACATGCTCCTTCGGATAGACGTGCGACCTATACTGCC
TGGATCATCATGACGGCGGCGATCGGCTTCCTGCTCGCGGTAGCGGTAATCATCCCTCTCCGCTTGGCTTTGGGAGCGGA
CGCGTTCGCACTCTGGGGATGGCGCGTTCCGTTCATTATCTCGATCGTTCTGCTGGGCGTGTCCCTGTGGATCAGACTTA
GGCTCGACGAATCGCCCGAGTTCAAGCGGATGAAGGCGGAGGGCAAGGCTTCGAAGTCTCCTCTGGCGGAGACCTTCGGA
ACCTGGAGATACGTCAAGGTCATCATTGTCGCGGCCCTCTGCATCCTGCCGGCTCAGGCAGTGATCTGGTATACGGGACA
ATTCTACACGCTGTTCTTCCTTACCAAGGTCCTCAAGGTTGAGAACCTTTCCGCAAACATGATGCTCATCATCGCCACCG
TGTTAACCGCGCCCCTATACGTCGTTTTCGGAAAACTCTCCGATAGGATTGGACGTAAACCTGTTTACATCGCGGGTTAC
CTCCTCGCAGCTCTGGTAACCATCCCGACATTCCACGGACTGACGCACTTTGCCAATCCTGCATTGGAACGTGCGCAGGC
GAACACTCCGATCACGATTGTTGCTGATCCCAATGACTGCTCGTTCCAGTTCAATCCCCTCGGGACGTCGAAATTCACTA
CCTCATGCGACGTTGGTATCAACGCTGTCGCGAACCTCGGCTTGAACTATCAAAGCCAGGACGCCGCCGCGGGGACGGTT
GCATCGGTTAAGGTGGGAGACCGCGTCATCGCGAGCTACGCCGCCGATGCTGCGGATGCGGCTTCTCAGAAGACGAGATT
GGAAGCGGAACTGAAGCAGGCCCTGGCAGAGGCTGGGTACCCGGTTGGAAGCGCCGACCCCGAAAGTGTGAACAGCCCTG
CGATCATAGCGTTGCTTTGCGTGCTTCTGGCGCTCGGCGCCATGGTTTTCGCGCCGACGACGACCTCGCTACTTGAGATG
TTCCCTTCCCGGATTCGGTATACGGCGATGTCCTTCCCCTACCATCTCAGCGCGGCGTGGTTCGGCGGCTTCCTGCCAGC
AACGGCGTTTGCGATCGTCGCTGCCACCGGCAACGTGTACTCGGGGCTTTATTACCCGGTTAGCATCGCGGCGGCCTGCA
TGGTCTTGAGCCTGCTCTTCGCACGGGAGACGCGCGGGACGGACATCTCCAAGGGCTGA

Upstream 100 bases:

>100_bases
GCGACGCGGGAGATGCTCCTGCGGCGACGGGAACGCGTGTCTTTGTATGAAGGCGGCATCAAGCGATGGGCGCATTTTAA
ACAACTGATGGGAGGATCAG

Downstream 100 bases:

>100_bases
CCAAAAATCGAAGGCGGGCCGAGTGGCTCGCCTTCAAGCCCTGCCACGATGGCTGTCAGTTCCGTCCCAAGAACACAGAA
CCTTTCCGGCTCCCACCACA

Product: major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTHVETMTQAQGISRRDRKVILAASLGTVFEFYDFFLIGLVATEIAKAFFSGVNPTAGFIFTLLGFAAGFMLRPFGAIVF
GRLGDLVGRKYTFLVTIVLMGGSTFLIGLLPAYATIGVAAPIAFVAMRMLQGLALGGEFGGAMVYVAEHAPSDRRATYTA
WIIMTAAIGFLLAVAVIIPLRLALGADAFALWGWRVPFIISIVLLGVSLWIRLRLDESPEFKRMKAEGKASKSPLAETFG
TWRYVKVIIVAALCILPAQAVIWYTGQFYTLFFLTKVLKVENLSANMMLIIATVLTAPLYVVFGKLSDRIGRKPVYIAGY
LLAALVTIPTFHGLTHFANPALERAQANTPITIVADPNDCSFQFNPLGTSKFTTSCDVGINAVANLGLNYQSQDAAAGTV
ASVKVGDRVIASYAADAADAASQKTRLEAELKQALAEAGYPVGSADPESVNSPAIIALLCVLLALGAMVFAPTTTSLLEM
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVAATGNVYSGLYYPVSIAAACMVLSLLFARETRGTDISKG

Sequences:

>Translated_552_residues
MTHVETMTQAQGISRRDRKVILAASLGTVFEFYDFFLIGLVATEIAKAFFSGVNPTAGFIFTLLGFAAGFMLRPFGAIVF
GRLGDLVGRKYTFLVTIVLMGGSTFLIGLLPAYATIGVAAPIAFVAMRMLQGLALGGEFGGAMVYVAEHAPSDRRATYTA
WIIMTAAIGFLLAVAVIIPLRLALGADAFALWGWRVPFIISIVLLGVSLWIRLRLDESPEFKRMKAEGKASKSPLAETFG
TWRYVKVIIVAALCILPAQAVIWYTGQFYTLFFLTKVLKVENLSANMMLIIATVLTAPLYVVFGKLSDRIGRKPVYIAGY
LLAALVTIPTFHGLTHFANPALERAQANTPITIVADPNDCSFQFNPLGTSKFTTSCDVGINAVANLGLNYQSQDAAAGTV
ASVKVGDRVIASYAADAADAASQKTRLEAELKQALAEAGYPVGSADPESVNSPAIIALLCVLLALGAMVFAPTTTSLLEM
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVAATGNVYSGLYYPVSIAAACMVLSLLFARETRGTDISKG
>Mature_551_residues
THVETMTQAQGISRRDRKVILAASLGTVFEFYDFFLIGLVATEIAKAFFSGVNPTAGFIFTLLGFAAGFMLRPFGAIVFG
RLGDLVGRKYTFLVTIVLMGGSTFLIGLLPAYATIGVAAPIAFVAMRMLQGLALGGEFGGAMVYVAEHAPSDRRATYTAW
IIMTAAIGFLLAVAVIIPLRLALGADAFALWGWRVPFIISIVLLGVSLWIRLRLDESPEFKRMKAEGKASKSPLAETFGT
WRYVKVIIVAALCILPAQAVIWYTGQFYTLFFLTKVLKVENLSANMMLIIATVLTAPLYVVFGKLSDRIGRKPVYIAGYL
LAALVTIPTFHGLTHFANPALERAQANTPITIVADPNDCSFQFNPLGTSKFTTSCDVGINAVANLGLNYQSQDAAAGTVA
SVKVGDRVIASYAADAADAASQKTRLEAELKQALAEAGYPVGSADPESVNSPAIIALLCVLLALGAMVFAPTTTSLLEMF
PSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVAATGNVYSGLYYPVSIAAACMVLSLLFARETRGTDISKG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.0609756097561, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788292, Length=326, Percent_Identity=34.3558282208589, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788942, Length=333, Percent_Identity=32.7327327327327, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1789941, Length=349, Percent_Identity=36.1031518624642, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=28.2700421940928, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082404, Length=328, Percent_Identity=25.3048780487805, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59021; Mature: 58890

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVETMTQAQGISRRDRKVILAASLGTVFEFYDFFLIGLVATEIAKAFFSGVNPTAGFI
CCCHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTLLGFAAGFMLRPFGAIVFGRLGDLVGRKYTFLVTIVLMGGSTFLIGLLPAYATIGVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
PIAFVAMRMLQGLALGGEFGGAMVYVAEHAPSDRRATYTAWIIMTAAIGFLLAVAVIIPL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLALGADAFALWGWRVPFIISIVLLGVSLWIRLRLDESPEFKRMKAEGKASKSPLAETFG
HHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCCHHHHHH
TWRYVKVIIVAALCILPAQAVIWYTGQFYTLFFLTKVLKVENLSANMMLIIATVLTAPLY
HHHHHHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVFGKLSDRIGRKPVYIAGYLLAALVTIPTFHGLTHFANPALERAQANTPITIVADPNDC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCC
SFQFNPLGTSKFTTSCDVGINAVANLGLNYQSQDAAAGTVASVKVGDRVIASYAADAADA
CEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
ASQKTRLEAELKQALAEAGYPVGSADPESVNSPAIIALLCVLLALGAMVFAPTTTSLLEM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVAATGNVYSGLYYPVSIAAACMVLSLLF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHH
ARETRGTDISKG
HHHCCCCCCCCC
>Mature Secondary Structure 
THVETMTQAQGISRRDRKVILAASLGTVFEFYDFFLIGLVATEIAKAFFSGVNPTAGFI
CCHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FTLLGFAAGFMLRPFGAIVFGRLGDLVGRKYTFLVTIVLMGGSTFLIGLLPAYATIGVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
PIAFVAMRMLQGLALGGEFGGAMVYVAEHAPSDRRATYTAWIIMTAAIGFLLAVAVIIPL
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLALGADAFALWGWRVPFIISIVLLGVSLWIRLRLDESPEFKRMKAEGKASKSPLAETFG
HHHHCCCHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCCHHHHHH
TWRYVKVIIVAALCILPAQAVIWYTGQFYTLFFLTKVLKVENLSANMMLIIATVLTAPLY
HHHHHHHHHHHHHHHHCCHHEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVFGKLSDRIGRKPVYIAGYLLAALVTIPTFHGLTHFANPALERAQANTPITIVADPNDC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEECCCCC
SFQFNPLGTSKFTTSCDVGINAVANLGLNYQSQDAAAGTVASVKVGDRVIASYAADAADA
CEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
ASQKTRLEAELKQALAEAGYPVGSADPESVNSPAIIALLCVLLALGAMVFAPTTTSLLEM
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
FPSRIRYTAMSFPYHLSAAWFGGFLPATAFAIVAATGNVYSGLYYPVSIAAACMVLSLLF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHH
ARETRGTDISKG
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]