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The map label for this gene is rutF [H]

Identifier: 209551974

GI number: 209551974

Start: 37812

End: 38936

Strand: Reverse

Name: rutF [H]

Synonym: Rleg2_6111

Alternate gene names: 209551974

Gene position: 38936-37812 (Counterclockwise)

Preceding gene: 209551975

Following gene: 209551973

Centisome position: 12.61

GC content: 57.07

Gene sequence:

>1125_bases
ATGAGCAACGATGTAAGCGATATCGCCCAGATTACGTCGGACTTCAAGAAGGCCATGCGGCGACTCGCGTCCACGGTGAG
CATCATCAGCACCGCGGATACAAACGGCAATCGCTATGGGATGACGGCGACGGCCGTGAATTCGCTTTCGATGGACCCGC
CGTCGTTGCTGATTTGTGTCAATCACAACGCCAGCATTTATACGCCGCTGGTTGGGCGGGGCAGGTTCTGCGTAAACGTG
TTGACCACGGATCACGAGGACCTCGTCGGCGCGTTCAGCGGAAAGCTCAAGGGCGATGCCCGTTTTCAGGTTGGCGAATG
GCGATCGGAAGCGACCGGAATGCCCTATCTTGACGGCGCGCAGTGCAATCTTTTTTGCGACATTGAAAGCGAAATCCCCT
TTGGCTCCCATTCGATAATCGTTGGGCGGCTGACAGATGTGCGGGTTGAGGAGGACATACGTCCGCTCATCTTCGCGGAC
GGCAGGCTCGGCACCACGCAGTCGCTTACCGGGCATGTCAGGCTGGTCTCGAACCTGTCCACTTTGACACAGTTTTTACC
GTCCGACCTGAGCAGTTTCCTATCGAAGTATCCGCTTATCGACATGCACGTCGAAGAGCAGATCAGCACGGACATCGTGA
CAGCCGTGGCTGGAAATGCCTTCGACATCGGTTTCATAGTCGAAGGGCCCAAAACGGGATCCCTCGAAGTCATTCCCTAC
CGCGACGATCGGCTCGTCCTTGCTGTTCCGGAAAATCATCCCCTTGCCGCCTTGGCGTCGACGAAATTCGAACAGACACT
TGAATACAGCTATGTCGGCCTCCATACCGGCAGCCAGATCAACCTGCAAATTCAGAAAGCCGCGGCCGACCTCGACATGC
CGTGGAAGTGCCGCATGCAGGTCCATACCTATGACGCGCTTGCCGCGATGGTGAAAGCCGGATTGGGCATTGGACTGCTT
CCCGAAAACATGGCGGGGGTGTTCTCGAAGGCCCTTGGCATCAAGGTCGTTCAAATAGAAGAGGACTGGACCCGGCGTCG
ACACGCGATCGTGTTGCGCTCATACGAAGGTTTGCAGGCCGCCGGCAAACGGCTGGTCGACCACCTCAAGCGCCAACTAC
CTTGA

Upstream 100 bases:

>100_bases
GAAACGGTTCCGATGCAGATCGCGGTCTGAAGAAGCAAGGCCCGGCTTCGTGTCGGGCCTACCTTGTGGTGCATGCGAAG
ACGTTAGGAAGGAATTCGGC

Downstream 100 bases:

>100_bases
GCGGGGAGGAGGGCACCTTATGAGTAATCTAATCGTCGGCATGAGCGGAAACTTTTCGCGTCCATCAAAAACCAGGACAT
TGGTGACCACGGTGGTTGAG

Product: flavin reductase domain protein FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSNDVSDIAQITSDFKKAMRRLASTVSIISTADTNGNRYGMTATAVNSLSMDPPSLLICVNHNASIYTPLVGRGRFCVNV
LTTDHEDLVGAFSGKLKGDARFQVGEWRSEATGMPYLDGAQCNLFCDIESEIPFGSHSIIVGRLTDVRVEEDIRPLIFAD
GRLGTTQSLTGHVRLVSNLSTLTQFLPSDLSSFLSKYPLIDMHVEEQISTDIVTAVAGNAFDIGFIVEGPKTGSLEVIPY
RDDRLVLAVPENHPLAALASTKFEQTLEYSYVGLHTGSQINLQIQKAAADLDMPWKCRMQVHTYDALAAMVKAGLGIGLL
PENMAGVFSKALGIKVVQIEEDWTRRRHAIVLRSYEGLQAAGKRLVDHLKRQLP

Sequences:

>Translated_374_residues
MSNDVSDIAQITSDFKKAMRRLASTVSIISTADTNGNRYGMTATAVNSLSMDPPSLLICVNHNASIYTPLVGRGRFCVNV
LTTDHEDLVGAFSGKLKGDARFQVGEWRSEATGMPYLDGAQCNLFCDIESEIPFGSHSIIVGRLTDVRVEEDIRPLIFAD
GRLGTTQSLTGHVRLVSNLSTLTQFLPSDLSSFLSKYPLIDMHVEEQISTDIVTAVAGNAFDIGFIVEGPKTGSLEVIPY
RDDRLVLAVPENHPLAALASTKFEQTLEYSYVGLHTGSQINLQIQKAAADLDMPWKCRMQVHTYDALAAMVKAGLGIGLL
PENMAGVFSKALGIKVVQIEEDWTRRRHAIVLRSYEGLQAAGKRLVDHLKRQLP
>Mature_373_residues
SNDVSDIAQITSDFKKAMRRLASTVSIISTADTNGNRYGMTATAVNSLSMDPPSLLICVNHNASIYTPLVGRGRFCVNVL
TTDHEDLVGAFSGKLKGDARFQVGEWRSEATGMPYLDGAQCNLFCDIESEIPFGSHSIIVGRLTDVRVEEDIRPLIFADG
RLGTTQSLTGHVRLVSNLSTLTQFLPSDLSSFLSKYPLIDMHVEEQISTDIVTAVAGNAFDIGFIVEGPKTGSLEVIPYR
DDRLVLAVPENHPLAALASTKFEQTLEYSYVGLHTGSQINLQIQKAAADLDMPWKCRMQVHTYDALAAMVKAGLGIGLLP
ENMAGVFSKALGIKVVQIEEDWTRRRHAIVLRSYEGLQAAGKRLVDHLKRQLP

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=146, Percent_Identity=32.1917808219178, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693105, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 40922; Mature: 40791

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDVSDIAQITSDFKKAMRRLASTVSIISTADTNGNRYGMTATAVNSLSMDPPSLLICV
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEECEEHHEECCCCCCCCEEEEEE
NHNASIYTPLVGRGRFCVNVLTTDHEDLVGAFSGKLKGDARFQVGEWRSEATGMPYLDGA
ECCCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCC
QCNLFCDIESEIPFGSHSIIVGRLTDVRVEEDIRPLIFADGRLGTTQSLTGHVRLVSNLS
CEEEEEEECCCCCCCCCEEEEEEEECEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
TLTQFLPSDLSSFLSKYPLIDMHVEEQISTDIVTAVAGNAFDIGFIVEGPKTGSLEVIPY
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEE
RDDRLVLAVPENHPLAALASTKFEQTLEYSYVGLHTGSQINLQIQKAAADLDMPWKCRMQ
CCCEEEEEECCCCCEEHHHHHHHHHHHCEEEEEEECCCEEEEEEEEECCCCCCCEEEEEE
VHTYDALAAMVKAGLGIGLLPENMAGVFSKALGIKVVQIEEDWTRRRHAIVLRSYEGLQA
EEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECCHHHCCCEEEEEECCCCHHH
AGKRLVDHLKRQLP
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SNDVSDIAQITSDFKKAMRRLASTVSIISTADTNGNRYGMTATAVNSLSMDPPSLLICV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEECEEHHEECCCCCCCCEEEEEE
NHNASIYTPLVGRGRFCVNVLTTDHEDLVGAFSGKLKGDARFQVGEWRSEATGMPYLDGA
ECCCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCCC
QCNLFCDIESEIPFGSHSIIVGRLTDVRVEEDIRPLIFADGRLGTTQSLTGHVRLVSNLS
CEEEEEEECCCCCCCCCEEEEEEEECEEECCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
TLTQFLPSDLSSFLSKYPLIDMHVEEQISTDIVTAVAGNAFDIGFIVEGPKTGSLEVIPY
HHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEE
RDDRLVLAVPENHPLAALASTKFEQTLEYSYVGLHTGSQINLQIQKAAADLDMPWKCRMQ
CCCEEEEEECCCCCEEHHHHHHHHHHHCEEEEEEECCCEEEEEEEEECCCCCCCEEEEEE
VHTYDALAAMVKAGLGIGLLPENMAGVFSKALGIKVVQIEEDWTRRRHAIVLRSYEGLQA
EEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECCHHHCCCEEEEEECCCCHHH
AGKRLVDHLKRQLP
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA