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The map label for this gene is rihB [H]

Identifier: 209550739

GI number: 209550739

Start: 3245891

End: 3246811

Strand: Direct

Name: rihB [H]

Synonym: Rleg2_3163

Alternate gene names: 209550739

Gene position: 3245891-3246811 (Clockwise)

Preceding gene: 209550738

Following gene: 209550740

Centisome position: 71.53

GC content: 66.88

Gene sequence:

>921_bases
ATGGGCGTCTGGATCGACACCGACATGGGCTTCGACGACATCGCCGCCATTCTGGTGGTGGCACAGTCGGATTACGAGAT
CGACGGCGTGTCGCTGGTTTTCGGCAATGCGCCGTTGCCGCAGGTGAGAAGCAATGCCGCCGGCGCCGCCAGCGCCTTCG
GCTGGAATTTCCCCATCCACACCGGTCGCGCCATGCCGGTTCTCGGCAAGCTCGAAACCGCCCAGGCGATCCTCGGCGCA
ACCGGCATCCCAACCATCGGCAAGAGCCTGCCGGAAGCATCAGCCCTTGCCGAGAGCGATGCCTTCGCAGCACTGTGTCG
CTGGCTGGACGGCCAGGGCCAGCGCCGCATCCTGGCGCTTGGCCCGCTCACCAATATCGCCGCCGTGGCACTCGCCCGCC
CCGATCTTGCCGCCCGTATCAGCGACCTCGTCTGGATGGGCGGCGGCGTCACGTCGGGCAATCACACGGCCTCTGCCGAG
TTCAATGCGCTCGCCGATCCCGAGGCTCTGTCGATCGTCATCGCCCATGGCCTGCCGCTGCGCATGGTCGATCTCGACCT
CTGCCGCAAGGTGCTGGCAAGGCCGGAGGATGCCGAGCCGGTGCGCAATGCCGGCGGCGCCAATGCCGAGCTGATCGCCG
ATCTGTTTGCAGGTTACATCCGCATCGGCACCAGCCGCGGCCGTCCCGGTATGGCGATCTACGATCCTTCTGCCGCGGTC
GCCTTCGTCGCTCCCGATATCGTCGGCTTTCGCCCCGCGCGCATCGACGTCGAGCTTCAGGGCAGCCTGACGCGTGGCCG
CACCGTCGTCGAGACCCGCGCCACGCATGCGACCTTCAACGCGCAATTCGCCGCCGAAATCGATGCCGAGAGGGCGCGCA
GGATCATTCTCGCCGCCCTGGTCAACGAGGCCCGCAAATGA

Upstream 100 bases:

>100_bases
GAGATCGATGCACGCCTGCCGCCGCACGAGATCGGCAGCGCGCTCGCCTTCGACCTGCCGGTTGCCGGCGCGGCCAATCT
CAAACGGTTCGGGTGAAATC

Downstream 100 bases:

>100_bases
ACGCCACCCCTCGCCAGGAACCCGCCGATCTCAACGATCCCGCTTTGCGCGCCCGCGCCGTTGCCGCCGCGCGCGGCGAC
GCCCCCTTCGATATGCTGAT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MGVWIDTDMGFDDIAAILVVAQSDYEIDGVSLVFGNAPLPQVRSNAAGAASAFGWNFPIHTGRAMPVLGKLETAQAILGA
TGIPTIGKSLPEASALAESDAFAALCRWLDGQGQRRILALGPLTNIAAVALARPDLAARISDLVWMGGGVTSGNHTASAE
FNALADPEALSIVIAHGLPLRMVDLDLCRKVLARPEDAEPVRNAGGANAELIADLFAGYIRIGTSRGRPGMAIYDPSAAV
AFVAPDIVGFRPARIDVELQGSLTRGRTVVETRATHATFNAQFAAEIDAERARRIILAALVNEARK

Sequences:

>Translated_306_residues
MGVWIDTDMGFDDIAAILVVAQSDYEIDGVSLVFGNAPLPQVRSNAAGAASAFGWNFPIHTGRAMPVLGKLETAQAILGA
TGIPTIGKSLPEASALAESDAFAALCRWLDGQGQRRILALGPLTNIAAVALARPDLAARISDLVWMGGGVTSGNHTASAE
FNALADPEALSIVIAHGLPLRMVDLDLCRKVLARPEDAEPVRNAGGANAELIADLFAGYIRIGTSRGRPGMAIYDPSAAV
AFVAPDIVGFRPARIDVELQGSLTRGRTVVETRATHATFNAQFAAEIDAERARRIILAALVNEARK
>Mature_305_residues
GVWIDTDMGFDDIAAILVVAQSDYEIDGVSLVFGNAPLPQVRSNAAGAASAFGWNFPIHTGRAMPVLGKLETAQAILGAT
GIPTIGKSLPEASALAESDAFAALCRWLDGQGQRRILALGPLTNIAAVALARPDLAARISDLVWMGGGVTSGNHTASAEF
NALADPEALSIVIAHGLPLRMVDLDLCRKVLARPEDAEPVRNAGGANAELIADLFAGYIRIGTSRGRPGMAIYDPSAAVA
FVAPDIVGFRPARIDVELQGSLTRGRTVVETRATHATFNAQFAAEIDAERARRIILAALVNEARK

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=273, Percent_Identity=28.5714285714286, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786871, Length=277, Percent_Identity=28.5198555956679, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786213, Length=300, Percent_Identity=29, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32565474, Length=236, Percent_Identity=30.9322033898305, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=300, Percent_Identity=27, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 32099; Mature: 31968

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVWIDTDMGFDDIAAILVVAQSDYEIDGVSLVFGNAPLPQVRSNAAGAASAFGWNFPIH
CCEEEECCCCHHHHEEEEEEECCCCCCCCEEEEECCCCCCHHHCCCCCCHHHCCCCCCCC
TGRAMPVLGKLETAQAILGATGIPTIGKSLPEASALAESDAFAALCRWLDGQGQRRILAL
CCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GPLTNIAAVALARPDLAARISDLVWMGGGVTSGNHTASAEFNALADPEALSIVIAHGLPL
CCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHEEEEEECCCCE
RMVDLDLCRKVLARPEDAEPVRNAGGANAELIADLFAGYIRIGTSRGRPGMAIYDPSAAV
EEECHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECCCCEE
AFVAPDIVGFRPARIDVELQGSLTRGRTVVETRATHATFNAQFAAEIDAERARRIILAAL
EEECCHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEECCEEEECCCHHHHHHHHHHHH
VNEARK
HHHHCC
>Mature Secondary Structure 
GVWIDTDMGFDDIAAILVVAQSDYEIDGVSLVFGNAPLPQVRSNAAGAASAFGWNFPIH
CEEEECCCCHHHHEEEEEEECCCCCCCCEEEEECCCCCCHHHCCCCCCHHHCCCCCCCC
TGRAMPVLGKLETAQAILGATGIPTIGKSLPEASALAESDAFAALCRWLDGQGQRRILAL
CCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GPLTNIAAVALARPDLAARISDLVWMGGGVTSGNHTASAEFNALADPEALSIVIAHGLPL
CCHHHHHHHHHCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCHHEEEEEECCCCE
RMVDLDLCRKVLARPEDAEPVRNAGGANAELIADLFAGYIRIGTSRGRPGMAIYDPSAAV
EEECHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHEECCCCCCCCEEEECCCCEE
AFVAPDIVGFRPARIDVELQGSLTRGRTVVETRATHATFNAQFAAEIDAERARRIILAAL
EEECCHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEECCEEEECCCHHHHHHHHHHHH
VNEARK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA