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The map label for this gene is pepP [C]

Identifier: 209550340

GI number: 209550340

Start: 2804421

End: 2805572

Strand: Direct

Name: pepP [C]

Synonym: Rleg2_2761

Alternate gene names: 209550340

Gene position: 2804421-2805572 (Clockwise)

Preceding gene: 209550339

Following gene: 209550341

Centisome position: 61.8

GC content: 64.06

Gene sequence:

>1152_bases
ATGGCACTGCACTTCGAAAAGGCCGAATTCGCAAGCCGGCTTGCGCGCCTCACCGAGAAGATGAAGGAGGAAAAGCTCGA
CGCCCTGCTGCTCTTCGCCCAGGAGAGCATGTACTGGCTGACCGGCTACGACACGTTCGGCTACTGCTTCTTCCAGACGC
TGGTCGTCAAGAGCGACGGCACCATGGCGTTGCTGACCCGCTCGGCCGATCTTCGCCAGGCTAGGCACACCTCGATCCTC
GAGGACATCCATATCTGGGTCGACCGGGTCAATGCCGATCCGACGCTCGACCTGAAGAACCTGCTGGTCGAGATGGATCT
GCTCGGCGCCCGCATCGGCGTCGAATATGACACGCACGGCATGACCGGCCGTATCTCGCGCCTGCTCGACGCGCAGTTGA
CCACCTTCGGCCAGATCACCGACGCTTCCTACCTCGTCAGTCGCCTGCGCCTTGTCAAAAGCCCGACCGAGGTTGCCTAT
GTCGAGCGCGCCGCCGTTCTCGCCGACGATGCGCTCGACGCCGCGATCCGGCTGACGAAGCCCGGCGCCGACGAGGCCGA
TATCCTCGCCGCCATGCAGGGCGCGATCTTTTCCGGCGGCGGCGACTATCCCGCCAACGAGTTCATCATCGGCTCCGGCG
CCGATGCCCTGCTTTGCCGTTACAAGGCCGGCCGCCGCAAGCTCGACGCCAGCGACCAGCTGACGCTCGAATGGGCCGGC
GCTTACGCGCATTACCACGCCGCCATGATGCGCACGATCGTCATCGGCGAGCCGATGCAACGCCACCGCGAACTCTATAA
TGCCTGCCGCGAAACCATCGAGGCGATCGAGACGGTGCTGAAGCCGGGCAACAGCTTCGGCGACGTCTTCGACATGCATG
CCAGGATCATGGACGAACGCGGCCTTGCCCGCCACCGGCTGAACGCCTGCGGTTATTCGCTCGGCGCCCGCTTCTCGCCC
TCCTGGATGGAGCATCAGATGTTCCATGTCGGCAATCCGCAGCCGATCGAGCCGAACATGTCGCTCTTCGTGCACATGAT
CATCGCCGATTCCGACACGGGCACGGCGATGACGCTCGGCCAGACCTATCTGACGACGGCGGATGCGCCGCGCGCGCTCT
CCCGCCATCCGCTCGATTTCATCGGTCTTTGA

Upstream 100 bases:

>100_bases
ACAGCGAGCGCTTCTTTTCCTACCGCAGGACGACACATCGCAAGGAGCCGGACTACGGCCGGCAGATTTCCGCGATATCA
ATCAGGGAGACGTGAGCAGA

Downstream 100 bases:

>100_bases
CCGATGAGAATCCGGAATTGACAGACATCAGCCGCCGCCCGCATAAATTCGGGCGGGGCTGATGCGAATGTGGGAAGGAC
GGATCAAGGGATGACGCGAG

Product: peptidase M24

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MALHFEKAEFASRLARLTEKMKEEKLDALLLFAQESMYWLTGYDTFGYCFFQTLVVKSDGTMALLTRSADLRQARHTSIL
EDIHIWVDRVNADPTLDLKNLLVEMDLLGARIGVEYDTHGMTGRISRLLDAQLTTFGQITDASYLVSRLRLVKSPTEVAY
VERAAVLADDALDAAIRLTKPGADEADILAAMQGAIFSGGGDYPANEFIIGSGADALLCRYKAGRRKLDASDQLTLEWAG
AYAHYHAAMMRTIVIGEPMQRHRELYNACRETIEAIETVLKPGNSFGDVFDMHARIMDERGLARHRLNACGYSLGARFSP
SWMEHQMFHVGNPQPIEPNMSLFVHMIIADSDTGTAMTLGQTYLTTADAPRALSRHPLDFIGL

Sequences:

>Translated_383_residues
MALHFEKAEFASRLARLTEKMKEEKLDALLLFAQESMYWLTGYDTFGYCFFQTLVVKSDGTMALLTRSADLRQARHTSIL
EDIHIWVDRVNADPTLDLKNLLVEMDLLGARIGVEYDTHGMTGRISRLLDAQLTTFGQITDASYLVSRLRLVKSPTEVAY
VERAAVLADDALDAAIRLTKPGADEADILAAMQGAIFSGGGDYPANEFIIGSGADALLCRYKAGRRKLDASDQLTLEWAG
AYAHYHAAMMRTIVIGEPMQRHRELYNACRETIEAIETVLKPGNSFGDVFDMHARIMDERGLARHRLNACGYSLGARFSP
SWMEHQMFHVGNPQPIEPNMSLFVHMIIADSDTGTAMTLGQTYLTTADAPRALSRHPLDFIGL
>Mature_382_residues
ALHFEKAEFASRLARLTEKMKEEKLDALLLFAQESMYWLTGYDTFGYCFFQTLVVKSDGTMALLTRSADLRQARHTSILE
DIHIWVDRVNADPTLDLKNLLVEMDLLGARIGVEYDTHGMTGRISRLLDAQLTTFGQITDASYLVSRLRLVKSPTEVAYV
ERAAVLADDALDAAIRLTKPGADEADILAAMQGAIFSGGGDYPANEFIIGSGADALLCRYKAGRRKLDASDQLTLEWAGA
YAHYHAAMMRTIVIGEPMQRHRELYNACRETIEAIETVLKPGNSFGDVFDMHARIMDERGLARHRLNACGYSLGARFSPS
WMEHQMFHVGNPQPIEPNMSLFVHMIIADSDTGTAMTLGQTYLTTADAPRALSRHPLDFIGL

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

Organism=Escherichia coli, GI1789275, Length=167, Percent_Identity=27.5449101796407, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 42629; Mature: 42498

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHFEKAEFASRLARLTEKMKEEKLDALLLFAQESMYWLTGYDTFGYCFFQTLVVKSDG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCHHHHHHHHHHHEECCC
TMALLTRSADLRQARHTSILEDIHIWVDRVNADPTLDLKNLLVEMDLLGARIGVEYDTHG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCC
MTGRISRLLDAQLTTFGQITDASYLVSRLRLVKSPTEVAYVERAAVLADDALDAAIRLTK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECC
PGADEADILAAMQGAIFSGGGDYPANEFIIGSGADALLCRYKAGRRKLDASDQLTLEWAG
CCCCHHHHHHHHHCCEECCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEHH
AYAHYHAAMMRTIVIGEPMQRHRELYNACRETIEAIETVLKPGNSFGDVFDMHARIMDER
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GLARHRLNACGYSLGARFSPSWMEHQMFHVGNPQPIEPNMSLFVHMIIADSDTGTAMTLG
CHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEC
QTYLTTADAPRALSRHPLDFIGL
CCEEECCCCCHHHHCCCCHHCCC
>Mature Secondary Structure 
ALHFEKAEFASRLARLTEKMKEEKLDALLLFAQESMYWLTGYDTFGYCFFQTLVVKSDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCHHHHHHHHHHHEECCC
TMALLTRSADLRQARHTSILEDIHIWVDRVNADPTLDLKNLLVEMDLLGARIGVEYDTHG
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCC
MTGRISRLLDAQLTTFGQITDASYLVSRLRLVKSPTEVAYVERAAVLADDALDAAIRLTK
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEECC
PGADEADILAAMQGAIFSGGGDYPANEFIIGSGADALLCRYKAGRRKLDASDQLTLEWAG
CCCCHHHHHHHHHCCEECCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEHH
AYAHYHAAMMRTIVIGEPMQRHRELYNACRETIEAIETVLKPGNSFGDVFDMHARIMDER
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GLARHRLNACGYSLGARFSPSWMEHQMFHVGNPQPIEPNMSLFVHMIIADSDTGTAMTLG
CHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEC
QTYLTTADAPRALSRHPLDFIGL
CCEEECCCCCHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]