Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 209550196

GI number: 209550196

Start: 2658905

End: 2660122

Strand: Direct

Name: mltB [H]

Synonym: Rleg2_2616

Alternate gene names: 209550196

Gene position: 2658905-2660122 (Clockwise)

Preceding gene: 209550195

Following gene: 209550197

Centisome position: 58.59

GC content: 65.68

Gene sequence:

>1218_bases
ATGCACCGCTCGCTCGCAAGCCGCTCTGCACTCGCCCTTCTGTTTGCCCTCGCGCTTGCCGGCGGCGCAGCCGCCCAGCA
GGCGCCCGACGCAGCCCCGGCCGCGCCCTGCGGCGGCGATCTGTCCGCTTTCCTTGAAGGTGTCAAGAAGGATGCAATTG
CCGCCGGCGCCAGTGCGGCAGCCGCCGACGCAGCCCTTGCCGGCGCCGAGATCGACCCTAAGGTTCTGAGCCGGGATCGA
GCCCAGGGCGTCTTCAAACAGACCTTCCTCGAATTCTCCCAGCGCACCGTCAGCCAGGCCCGCCTCGACATCGGCCGCCA
GAAGATGAAGCAATATGCCGACGTCTTTGCCCGCGCCGAGCAGGAATTCGGTGTTCCCGCAGGCGTGATCACCGCCTTCT
GGGCGATGGAAACCGATTTCGGCGCCGTCCAGGGCGATTTCAACACCCGCAATGCCCTGGTGACACTGTCGCATGACTGC
CGCCGCCCGGAGCTCTTCCGCCCGCAGCTGATCGCCCTCATCGAGATGGTCCAGCACGGCGATCTCGACCCCGCCGCCAA
TACCGGCGCCTGGGCCGGCGAGATCGGCCAGGTTCAGATGCTGCCGCGCGACATCATCGCCTATGGCATGGACGGCGACG
GCGACGGCCATATCCGCCTGAAGCAGAGCGGCCCGGACGCCATCCTGACGGCCGCGAAATTCATCCAGCATCTCGGCTTC
GAGCGCGGCCAGCCCTGGCTGCAGGAAGTCACCCTGCCCGATAACCTGCCCTGGGAGAAATCCGGCCTTGGCGGCACGAT
GACGGCAGGTGAATGGTTCACGCTCGGCGTCAAGCCGCGCGACGGCAACACCGCCTTCGGCAACCTGGAAGGCGATCTGG
TGCTGCCGCAGGGCCGCATGGGACCGGCCTTCATCGCCTACCCGAATTTCAAGATTTATCTCGAATGGAATAAGTCGTTC
ATCTACACGACCTCGGCCGCCTATTTCGGGACCCGACTTTCCGGCGCTCCGACCTATCTCAAGGGTGTGCCGGAACAGGG
GCTCGCCAACGACCAGATGAAGACGCTGCAGACCAAGCTGCAGTCGCTCGGTCATGATGTCGGCGAGATCGACGGTATTC
TCGGCTCCGGCACGCGTGTCGCGATCCAGAAGGAGCAGCAGCGCCTCGGCATGCCGGCCGACGGCTGGGCAACGCCGGCC
CTCCTCAACGCTCTCTGA

Upstream 100 bases:

>100_bases
CCAAGGATAGAAATTCTTAGTAACGTCTTATGATGTTTCTTGCTTCCGTCACATTCCTCTGTGAAAGCGACGGCAATCTG
TTTGATATAAGGACCGCATC

Downstream 100 bases:

>100_bases
TCGTCGCAGACACCTCACCGCCACCTGTTTCGCAAAAAACGGGTGGCGCGGTCAGCCGTATGGCCTAAGCATCAGTCCTG
GAAACCGCTCCCGGTTTTCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MHRSLASRSALALLFALALAGGAAAQQAPDAAPAAPCGGDLSAFLEGVKKDAIAAGASAAAADAALAGAEIDPKVLSRDR
AQGVFKQTFLEFSQRTVSQARLDIGRQKMKQYADVFARAEQEFGVPAGVITAFWAMETDFGAVQGDFNTRNALVTLSHDC
RRPELFRPQLIALIEMVQHGDLDPAANTGAWAGEIGQVQMLPRDIIAYGMDGDGDGHIRLKQSGPDAILTAAKFIQHLGF
ERGQPWLQEVTLPDNLPWEKSGLGGTMTAGEWFTLGVKPRDGNTAFGNLEGDLVLPQGRMGPAFIAYPNFKIYLEWNKSF
IYTTSAAYFGTRLSGAPTYLKGVPEQGLANDQMKTLQTKLQSLGHDVGEIDGILGSGTRVAIQKEQQRLGMPADGWATPA
LLNAL

Sequences:

>Translated_405_residues
MHRSLASRSALALLFALALAGGAAAQQAPDAAPAAPCGGDLSAFLEGVKKDAIAAGASAAAADAALAGAEIDPKVLSRDR
AQGVFKQTFLEFSQRTVSQARLDIGRQKMKQYADVFARAEQEFGVPAGVITAFWAMETDFGAVQGDFNTRNALVTLSHDC
RRPELFRPQLIALIEMVQHGDLDPAANTGAWAGEIGQVQMLPRDIIAYGMDGDGDGHIRLKQSGPDAILTAAKFIQHLGF
ERGQPWLQEVTLPDNLPWEKSGLGGTMTAGEWFTLGVKPRDGNTAFGNLEGDLVLPQGRMGPAFIAYPNFKIYLEWNKSF
IYTTSAAYFGTRLSGAPTYLKGVPEQGLANDQMKTLQTKLQSLGHDVGEIDGILGSGTRVAIQKEQQRLGMPADGWATPA
LLNAL
>Mature_405_residues
MHRSLASRSALALLFALALAGGAAAQQAPDAAPAAPCGGDLSAFLEGVKKDAIAAGASAAAADAALAGAEIDPKVLSRDR
AQGVFKQTFLEFSQRTVSQARLDIGRQKMKQYADVFARAEQEFGVPAGVITAFWAMETDFGAVQGDFNTRNALVTLSHDC
RRPELFRPQLIALIEMVQHGDLDPAANTGAWAGEIGQVQMLPRDIIAYGMDGDGDGHIRLKQSGPDAILTAAKFIQHLGF
ERGQPWLQEVTLPDNLPWEKSGLGGTMTAGEWFTLGVKPRDGNTAFGNLEGDLVLPQGRMGPAFIAYPNFKIYLEWNKSF
IYTTSAAYFGTRLSGAPTYLKGVPEQGLANDQMKTLQTKLQSLGHDVGEIDGILGSGTRVAIQKEQQRLGMPADGWATPA
LLNAL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=156, Percent_Identity=32.0512820512821, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43242; Mature: 43242

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRSLASRSALALLFALALAGGAAAQQAPDAAPAAPCGGDLSAFLEGVKKDAIAAGASAA
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
AADAALAGAEIDPKVLSRDRAQGVFKQTFLEFSQRTVSQARLDIGRQKMKQYADVFARAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFGVPAGVITAFWAMETDFGAVQGDFNTRNALVTLSHDCRRPELFRPQLIALIEMVQHG
HHHCCCHHHHHHHHHHCCCCCCEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
DLDPAANTGAWAGEIGQVQMLPRDIIAYGMDGDGDGHIRLKQSGPDAILTAAKFIQHLGF
CCCCCCCCCCCCCCCCCEEECCHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
ERGQPWLQEVTLPDNLPWEKSGLGGTMTAGEWFTLGVKPRDGNTAFGNLEGDLVLPQGRM
CCCCCHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCEECCCCCCEECCCCCC
GPAFIAYPNFKIYLEWNKSFIYTTSAAYFGTRLSGAPTYLKGVPEQGLANDQMKTLQTKL
CCEEEECCCEEEEEEECCEEEEEECHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHH
QSLGHDVGEIDGILGSGTRVAIQKEQQRLGMPADGWATPALLNAL
HHHCCCHHHHCCCCCCCCEEEEEEHHHHCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MHRSLASRSALALLFALALAGGAAAQQAPDAAPAAPCGGDLSAFLEGVKKDAIAAGASAA
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHH
AADAALAGAEIDPKVLSRDRAQGVFKQTFLEFSQRTVSQARLDIGRQKMKQYADVFARAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QEFGVPAGVITAFWAMETDFGAVQGDFNTRNALVTLSHDCRRPELFRPQLIALIEMVQHG
HHHCCCHHHHHHHHHHCCCCCCEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
DLDPAANTGAWAGEIGQVQMLPRDIIAYGMDGDGDGHIRLKQSGPDAILTAAKFIQHLGF
CCCCCCCCCCCCCCCCCEEECCHHHHEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCC
ERGQPWLQEVTLPDNLPWEKSGLGGTMTAGEWFTLGVKPRDGNTAFGNLEGDLVLPQGRM
CCCCCHHHHCCCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCEECCCCCCEECCCCCC
GPAFIAYPNFKIYLEWNKSFIYTTSAAYFGTRLSGAPTYLKGVPEQGLANDQMKTLQTKL
CCEEEECCCEEEEEEECCEEEEEECHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHH
QSLGHDVGEIDGILGSGTRVAIQKEQQRLGMPADGWATPALLNAL
HHHCCCHHHHCCCCCCCCEEEEEEHHHHCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]