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The map label for this gene is ubiE [H]

Identifier: 209549887

GI number: 209549887

Start: 2357808

End: 2358980

Strand: Direct

Name: ubiE [H]

Synonym: Rleg2_2303

Alternate gene names: 209549887

Gene position: 2357808-2358980 (Clockwise)

Preceding gene: 209549886

Following gene: 209549888

Centisome position: 51.96

GC content: 56.69

Gene sequence:

>1173_bases
ATGAACCTGCTCGCCCGCCCCTTCACCGTCCATTTCGATCCGACAAATCAGGAGCTGCTGCTGTCCGGCAAGATGCGGCC
CGAAAGTCCAAACGAGATTGCCGATGCTCTCAAGCTGGTGCGGCAAAGTTTGGAAAAATACAGCGGCGTCGTTTACCTGA
ATGTCAAACGTCTGACGCACATCAACATGACGGCCTTTAAGGCGCTCGCCGATATTCTGGCGGCACACTGCCGGACCAGG
CCGGAGCGTAGGATCGTCATCATTACGTCGAGCGTGGTGGCCTGGTCGACCTCATTGTTCCAGACCTTGGCGGCATCACA
ACCAAACCTGTCGGTTGAAGTCTACGATTCGGCGTTTTATCCGGGCCAGACCTTTGTCGAAGATGAGAGCTTCATTCCCA
TCCTGCGTACCCAAACGAAAATGACATGGAGGCACGAACGCGAGCTGCTGCGGCACCACGGCCTGCGCAACGGTCTTGTC
ATGGCGGATATCTGTTGCGGAATTGGTGATTTCGCCGCCCTCGTTCAACGGGAATTCAAACCGCAACGGCTTGTGGCGCT
CGATCATTCCGTACGCAGCCTCGACTATGCCCGCAGGGTCGCCGCCGACTTCGATCTGCAGGGGATAGAATATACCTATG
GCGACGCCTCTCAGATGCTGCTTCGGGATAATCAATTCGACTTTGTGACCTGCCGCCATTCGCTGCAGATTTTCAACCGT
CCCGAGATGCTTCTGCGTGAGCTTTACCGCATCTGCAAACCCGGCGGCCGTGTCTACATCACCAACGAGAAAAATTCGCA
TTGCCTGGGCGAGCCGCATGCGGAAACGATCAAATGGACGTACGAGGAAGTCGCCAAACTGTTCAGGTACTTCGACATGG
ATGTCGAGATGGGGCCGAAAGGTCGGCATCTGCTTGCCGATGCTGGCTTCGATGCCATCAAGGTCGATTGCTTTATGGTG
ACGAACCTCGACGGGGATCCTCAGGACTTCGCCGACATCATCGAGGCCTGGGAAAATGTCTACGCCGGTGAGATGGCGGT
CCGGCGCGGCGACTCGGTGGACTTTATCCGCAGATTCCGGCAAGGGTTCAAGGATCACGTCTTTGCCGCCCTTCACCCCA
AAGGTTATGCAGGCTGGCCGATCTGGGCGGCTTCCGGGCGAAAGCCACAATGA

Upstream 100 bases:

>100_bases
AAGATCTGGCCACCATATTCCGCGCCGCTATCCGAATTGAGCAAGACGGTGCTGACGGAATGCAGATCATCGCGGATTTC
CCCTCGGCCGAGGATTTCCA

Downstream 100 bases:

>100_bases
TGGGGGGGCGATCACTGAAAAGACCGGCAGGCATAGGCTTTCGGGCAAAGTTCATCCTGGTCGTCGGCGGAGCCGTCCTC
TTCGACCTGCTGGTCAGCGG

Product: type 11 methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNLLARPFTVHFDPTNQELLLSGKMRPESPNEIADALKLVRQSLEKYSGVVYLNVKRLTHINMTAFKALADILAAHCRTR
PERRIVIITSSVVAWSTSLFQTLAASQPNLSVEVYDSAFYPGQTFVEDESFIPILRTQTKMTWRHERELLRHHGLRNGLV
MADICCGIGDFAALVQREFKPQRLVALDHSVRSLDYARRVAADFDLQGIEYTYGDASQMLLRDNQFDFVTCRHSLQIFNR
PEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAETIKWTYEEVAKLFRYFDMDVEMGPKGRHLLADAGFDAIKVDCFMV
TNLDGDPQDFADIIEAWENVYAGEMAVRRGDSVDFIRRFRQGFKDHVFAALHPKGYAGWPIWAASGRKPQ

Sequences:

>Translated_390_residues
MNLLARPFTVHFDPTNQELLLSGKMRPESPNEIADALKLVRQSLEKYSGVVYLNVKRLTHINMTAFKALADILAAHCRTR
PERRIVIITSSVVAWSTSLFQTLAASQPNLSVEVYDSAFYPGQTFVEDESFIPILRTQTKMTWRHERELLRHHGLRNGLV
MADICCGIGDFAALVQREFKPQRLVALDHSVRSLDYARRVAADFDLQGIEYTYGDASQMLLRDNQFDFVTCRHSLQIFNR
PEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAETIKWTYEEVAKLFRYFDMDVEMGPKGRHLLADAGFDAIKVDCFMV
TNLDGDPQDFADIIEAWENVYAGEMAVRRGDSVDFIRRFRQGFKDHVFAALHPKGYAGWPIWAASGRKPQ
>Mature_390_residues
MNLLARPFTVHFDPTNQELLLSGKMRPESPNEIADALKLVRQSLEKYSGVVYLNVKRLTHINMTAFKALADILAAHCRTR
PERRIVIITSSVVAWSTSLFQTLAASQPNLSVEVYDSAFYPGQTFVEDESFIPILRTQTKMTWRHERELLRHHGLRNGLV
MADICCGIGDFAALVQREFKPQRLVALDHSVRSLDYARRVAADFDLQGIEYTYGDASQMLLRDNQFDFVTCRHSLQIFNR
PEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAETIKWTYEEVAKLFRYFDMDVEMGPKGRHLLADAGFDAIKVDCFMV
TNLDGDPQDFADIIEAWENVYAGEMAVRRGDSVDFIRRFRQGFKDHVFAALHPKGYAGWPIWAASGRKPQ

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014122
- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 44684; Mature: 44684

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLARPFTVHFDPTNQELLLSGKMRPESPNEIADALKLVRQSLEKYSGVVYLNVKRLTH
CCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHEE
INMTAFKALADILAAHCRTRPERRIVIITSSVVAWSTSLFQTLAASQPNLSVEVYDSAFY
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
PGQTFVEDESFIPILRTQTKMTWRHERELLRHHGLRNGLVMADICCGIGDFAALVQREFK
CCCCEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCC
PQRLVALDHSVRSLDYARRVAADFDLQGIEYTYGDASQMLLRDNQFDFVTCRHSLQIFNR
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCEEEEHHHHHHCCC
PEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAETIKWTYEEVAKLFRYFDMDVEMGPK
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHEECHHHHHHHHHHHCCCCCCCCC
GRHLLADAGFDAIKVDCFMVTNLDGDPQDFADIIEAWENVYAGEMAVRRGDSVDFIRRFR
CCEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QGFKDHVFAALHPKGYAGWPIWAASGRKPQ
HHHHHCEEEEECCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MNLLARPFTVHFDPTNQELLLSGKMRPESPNEIADALKLVRQSLEKYSGVVYLNVKRLTH
CCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHEE
INMTAFKALADILAAHCRTRPERRIVIITSSVVAWSTSLFQTLAASQPNLSVEVYDSAFY
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCCC
PGQTFVEDESFIPILRTQTKMTWRHERELLRHHGLRNGLVMADICCGIGDFAALVQREFK
CCCCEECCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHCC
PQRLVALDHSVRSLDYARRVAADFDLQGIEYTYGDASQMLLRDNQFDFVTCRHSLQIFNR
CCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHCCCCCCEEEEHHHHHHCCC
PEMLLRELYRICKPGGRVYITNEKNSHCLGEPHAETIKWTYEEVAKLFRYFDMDVEMGPK
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHEECHHHHHHHHHHHCCCCCCCCC
GRHLLADAGFDAIKVDCFMVTNLDGDPQDFADIIEAWENVYAGEMAVRRGDSVDFIRRFR
CCEEEECCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QGFKDHVFAALHPKGYAGWPIWAASGRKPQ
HHHHHCEEEEECCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]