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The map label for this gene is thcD [H]

Identifier: 209549883

GI number: 209549883

Start: 2353365

End: 2354570

Strand: Direct

Name: thcD [H]

Synonym: Rleg2_2299

Alternate gene names: 209549883

Gene position: 2353365-2354570 (Clockwise)

Preceding gene: 209549882

Following gene: 209549884

Centisome position: 51.86

GC content: 65.67

Gene sequence:

>1206_bases
ATGGACGGTATCGTCATCATCGGTGCGGGCGAAGCCGGCACCCGGGCAGCCTTTGCACTACGTGAAGCCGGTTATTCCAG
GCCCGTCACGCTGGTCGGAGCCGAGCCGCATTTACCCTATGAACGACCTCCCCTGTCGAAGACCGTGAACGATGCGGTGC
AGATGAAGCTGATCTGCGCGGCAGGCGCGCTGGAAGCGGCCGGCATTGCCTATCTCAAGGGCCTGTCGGCCGCCGAGCTG
GATGCCGGCGCCGGAACGGTTGGTTTGAGCGACGGACGGGTGCTGCGCTACGAAAAGCTGCTTCTGGCAACCGGCGCGCG
GCCGAGGCGGCTCACATGCCCCGGTGCGGCGCATGCACTCGACTTCCGCACCCACACTGAGGCCGAAGCGATCTTCTCCC
GTGTTGCGCCGGGCCGGAGCGTGGCGATCATCGGCGCCGGTCTGATCGGGATGGAACTTGCAGCGGTGCTCCGCGGAAAG
GACGTCGCGGTCAACGTGATCGAAGCCGCCCCGAAACCTTTGGGCCGCGCCGTCCCTGCCCGCTTTGCCGAAAAGCTGCA
CGTCAGACATGTTGCGGAAGGTGTGCGCTTTCACCTCGATCGAGGCGTGACGGCGATCGACGGCGACGGCGTTACCCTGA
CCGACGGCAGCCTGGTGCATGCCGATCTCGTCGTCAGCGCCATCGGTGTGGTACCCGACATCGCCTTGGCGGAAGCAGCG
GGACTGGCCACGGGCAACGGCATTCTGACGGACGCCTATCTTCGCACCAGCGCACCGAACGTCTTCGCCGCAGGCGATTG
CGCGGCGGTGGCTGAGCCCGGTGGCGGACATGTTCGCTATGAAAGCTGGCGGAACGCCAGGACGCAGGCCGAAACTGCGG
CGCGAAACATGGCGGGTGCCGCCGAAACCTTCGGCGCCCTTCCCTGGTTCTGGTCCGACCAGTACGATCTCGGCCTCCAG
GTGGCAGGATTGCCGCAGTCCGCCCATCGAACCGTCCAACGTTTCGCCGCGGAAGGCGAGCTCGAATTTTATCTCGATGA
CGAGCGCCTCGTGGCAGCCGCCGGCCTTGGCATGGGCAATGGCCTGGCCAGGGACATCAAGCTCGCCGAGATGCTGATTG
CCGCAGGCATCAGCCCCAGCCCCGCAGAACTGGCAGACCCGGGCGTAAACCTCAAAATGCTTCTGAAGAGCGCGCGGGCC
GCATGA

Upstream 100 bases:

>100_bases
GTGGCTCTGGCTTGATGCATGTCGCCCGGACCTGCGCAGCGCTTCAGGCGTTGGTTTTTATGTCCTCACGAGCGCCGGCC
GCTCTTTCCACGGAGACAAC

Downstream 100 bases:

>100_bases
CACAGAGGCTGCTGATCTTCCAATCGCTCTGGGCCATGGAGCGTCGGCACACGGATGGGCTGGAGCGCACACTCGACGAG
AACATCGCGATGATCTCCGA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDGIVIIGAGEAGTRAAFALREAGYSRPVTLVGAEPHLPYERPPLSKTVNDAVQMKLICAAGALEAAGIAYLKGLSAAEL
DAGAGTVGLSDGRVLRYEKLLLATGARPRRLTCPGAAHALDFRTHTEAEAIFSRVAPGRSVAIIGAGLIGMELAAVLRGK
DVAVNVIEAAPKPLGRAVPARFAEKLHVRHVAEGVRFHLDRGVTAIDGDGVTLTDGSLVHADLVVSAIGVVPDIALAEAA
GLATGNGILTDAYLRTSAPNVFAAGDCAAVAEPGGGHVRYESWRNARTQAETAARNMAGAAETFGALPWFWSDQYDLGLQ
VAGLPQSAHRTVQRFAAEGELEFYLDDERLVAAAGLGMGNGLARDIKLAEMLIAAGISPSPAELADPGVNLKMLLKSARA
A

Sequences:

>Translated_401_residues
MDGIVIIGAGEAGTRAAFALREAGYSRPVTLVGAEPHLPYERPPLSKTVNDAVQMKLICAAGALEAAGIAYLKGLSAAEL
DAGAGTVGLSDGRVLRYEKLLLATGARPRRLTCPGAAHALDFRTHTEAEAIFSRVAPGRSVAIIGAGLIGMELAAVLRGK
DVAVNVIEAAPKPLGRAVPARFAEKLHVRHVAEGVRFHLDRGVTAIDGDGVTLTDGSLVHADLVVSAIGVVPDIALAEAA
GLATGNGILTDAYLRTSAPNVFAAGDCAAVAEPGGGHVRYESWRNARTQAETAARNMAGAAETFGALPWFWSDQYDLGLQ
VAGLPQSAHRTVQRFAAEGELEFYLDDERLVAAAGLGMGNGLARDIKLAEMLIAAGISPSPAELADPGVNLKMLLKSARA
A
>Mature_401_residues
MDGIVIIGAGEAGTRAAFALREAGYSRPVTLVGAEPHLPYERPPLSKTVNDAVQMKLICAAGALEAAGIAYLKGLSAAEL
DAGAGTVGLSDGRVLRYEKLLLATGARPRRLTCPGAAHALDFRTHTEAEAIFSRVAPGRSVAIIGAGLIGMELAAVLRGK
DVAVNVIEAAPKPLGRAVPARFAEKLHVRHVAEGVRFHLDRGVTAIDGDGVTLTDGSLVHADLVVSAIGVVPDIALAEAA
GLATGNGILTDAYLRTSAPNVFAAGDCAAVAEPGGGHVRYESWRNARTQAETAARNMAGAAETFGALPWFWSDQYDLGLQ
VAGLPQSAHRTVQRFAAEGELEFYLDDERLVAAAGLGMGNGLARDIKLAEMLIAAGISPSPAELADPGVNLKMLLKSARA
A

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=387, Percent_Identity=29.9741602067183, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI65787454, Length=372, Percent_Identity=30.1075268817204, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI21389617, Length=387, Percent_Identity=29.9741602067183, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI4757732, Length=358, Percent_Identity=30.1675977653631, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI22202629, Length=358, Percent_Identity=30.1675977653631, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1788892, Length=400, Percent_Identity=34.75, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=31.5217391304348, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789765, Length=279, Percent_Identity=29.0322580645161, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787352, Length=239, Percent_Identity=29.7071129707113, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789915, Length=191, Percent_Identity=29.8429319371728, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=373, Percent_Identity=27.0777479892761, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564386, Length=360, Percent_Identity=29.4444444444444, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24639257, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281359715, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281359713, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24639250, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18543267, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24639252, Length=405, Percent_Identity=24.6913580246914, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585130, Length=405, Percent_Identity=23.2098765432099, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24581020, Length=385, Percent_Identity=25.974025974026, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28573993, Length=385, Percent_Identity=25.974025974026, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 41470; Mature: 41470

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIVIIGAGEAGTRAAFALREAGYSRPVTLVGAEPHLPYERPPLSKTVNDAVQMKLICA
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEC
AGALEAAGIAYLKGLSAAELDAGAGTVGLSDGRVLRYEKLLLATGARPRRLTCPGAAHAL
CCCHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEEHHHHHHHCCCCCCEEECCCCHHEE
DFRTHTEAEAIFSRVAPGRSVAIIGAGLIGMELAAVLRGKDVAVNVIEAAPKPLGRAVPA
ECCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEHHCCCCCCHHHHH
RFAEKLHVRHVAEGVRFHLDRGVTAIDGDGVTLTDGSLVHADLVVSAIGVVPDIALAEAA
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCEEHHHHHHHHHHHCCCHHHHHHC
GLATGNGILTDAYLRTSAPNVFAAGDCAAVAEPGGGHVRYESWRNARTQAETAARNMAGA
CCCCCCCCEEEHHHHCCCCCEEECCCCCEEECCCCCCEEEHHHHCHHHHHHHHHHHHCCH
AETFGALPWFWSDQYDLGLQVAGLPQSAHRTVQRFAAEGELEFYLDDERLVAAAGLGMGN
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCC
GLARDIKLAEMLIAAGISPSPAELADPGVNLKMLLKSARAA
CHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MDGIVIIGAGEAGTRAAFALREAGYSRPVTLVGAEPHLPYERPPLSKTVNDAVQMKLICA
CCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEC
AGALEAAGIAYLKGLSAAELDAGAGTVGLSDGRVLRYEKLLLATGARPRRLTCPGAAHAL
CCCHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEEHHHHHHHCCCCCCEEECCCCHHEE
DFRTHTEAEAIFSRVAPGRSVAIIGAGLIGMELAAVLRGKDVAVNVIEAAPKPLGRAVPA
ECCCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEHHCCCCCCHHHHH
RFAEKLHVRHVAEGVRFHLDRGVTAIDGDGVTLTDGSLVHADLVVSAIGVVPDIALAEAA
HHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCEEHHHHHHHHHHHCCCHHHHHHC
GLATGNGILTDAYLRTSAPNVFAAGDCAAVAEPGGGHVRYESWRNARTQAETAARNMAGA
CCCCCCCCEEEHHHHCCCCCEEECCCCCEEECCCCCCEEEHHHHCHHHHHHHHHHHHCCH
AETFGALPWFWSDQYDLGLQVAGLPQSAHRTVQRFAAEGELEFYLDDERLVAAAGLGMGN
HHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCC
GLARDIKLAEMLIAAGISPSPAELADPGVNLKMLLKSARAA
CHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]