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The map label for this gene is pyrG [H]

Identifier: 209549426

GI number: 209549426

Start: 1879947

End: 1881575

Strand: Reverse

Name: pyrG [H]

Synonym: Rleg2_1832

Alternate gene names: 209549426

Gene position: 1881575-1879947 (Counterclockwise)

Preceding gene: 209549427

Following gene: 209549425

Centisome position: 41.46

GC content: 62.86

Gene sequence:

>1629_bases
ATGGCGCGATACGTATTCATCACTGGCGGCGTGGTTTCCTCTCTCGGAAAAGGAATTGCGGCCGCGGCTCTCGGAGCGTT
GCTGCAGGCCCGTGGATATCGGGTACGGCTTCGCAAGCTCGACCCCTATCTGAACGTGGACCCGGGCACGATGAGCCCGA
CCCAGCACGGCGAGGTCTTCGTCACCGACGACGGCGCGGAAACCGATCTCGATCTCGGCCACTATGAACGCTTCACGGGG
CGTTCGGCGACCAAGACCGACAACATCACCACCGGCCGCATCTACAAGAACATCATCGACAAGGAACGGCGCGGCGACTA
TCTCGGCGCGACGGTGCAGGTCATTCCGCACGTCACCAACGAGATCAAGGATTTCGTCATCGAGGGCAATGACGACTACG
ACTTCGTCATCTGCGAGATCGGCGGCACGGTCGGCGACATCGAGGCGATGCCCTTCATGGAGGCGATCCGCCAGCTCGGC
AACGACCTGCCGCGCGGCACCGCGGTCTACGTCCACCTGACGCTGATGCCCTATATTCCGGCCGCCGGCGAGCTCAAGAC
CAAGCCGACCCAGCATTCGGTCAAGGAACTGCAGGCGCTCGGCATTCATCCCGATATTCTGCTGGTGCGCGCCGACCGCG
AAATTCCGGAAGCCGAGCGCCGCAAGCTTTCGCTGTTCTGCAACGTGCGCCCGTCCGCCGTCATCCAGGCGCTCGACGTC
GCCAACATCTACGATGTGCCGATTGCCTACCACAAGGAAGGCCTTGATGACGAAGTGCTGGCAGCCTTCGGCATCGAGCC
GGCGCCGAAGCCGCGTCTCGACCCGTGGGAAGAGGTCTGCAACCGTATCCGCACGCCTGAGGGCGAGGTGACGATCGCGA
TCGTCGGCAAATATACCGGCCTCAAGGATGCCTATAAATCGCTGATCGAGGCGCTGCACCACGGCGGCATCGCCAATCGC
GTCAAGGTCAAGCTCGAATGGATCGAGTCCGAGGTCTTCGAAAAGGAAGATCCGGCGCCGTACCTCGAAAAGGTGCACGG
CATCCTCGTGCCGGGCGGCTTCGGCGAACGCGGTTCGGAAGGCAAGATCCACGCAGCGCGTTTCGCCCGCGAGCGCAAGG
TGCCGTATTTCGGCATCTGCTTCGGCATGCAGATGGCCGTCATCGAGGCGGCGCGCAATCTCGCCGACGTATCCGGCGCC
TCCTCGACCGAATTCGGCCCGACCAAGGAGCCGGTGGTCGGCCTGATGACCGAATGGGTCAAGGGCAACGAGTTGCAGAA
GCGCACGGCAGCCGGCGATCTCGGCGGCACGATGCGCCTCGGCGCCTATAAGGCGGCGCTGAAGAAGGGCACGAAGATCT
CGGATATCTACGGTTCCACCGATATTTCCGAGCGGCATCGCCACCGCTACGAGGTCAATGTCGACTACAAGGACCGGCTC
GAGAGCTGCGGCCTCGTCTTCTCCGGCATGTCGCCGGATGGTGTGCTGCCGGAGACGATCGAATATCCGGATCACCCGTG
GTTCATCGGCGTGCAGTATCACCCGGAACTGAAGAGCCGGCCGCTCGACCCGCATCCGCTGTTTGCCAGCTTCATCGAAG
CGGCGACGGAACAGAGCCGCCTCGTCTAA

Upstream 100 bases:

>100_bases
GTTTTCTTTTGTTCAGATTCCTTCGCCACGCTCCGAAAATTCCGGAAAAGAGTTTTTGGGGCTGGTGGAATCGTTTTTGA
AAAGGTATCCGGTGAATCCC

Downstream 100 bases:

>100_bases
TCACGATGAAGATCCAGATCGCGCGACGTCAAGCGCGATCTGGGCAAGTGCTTGTGGTGCTCTATATCGCCGGCATGGCC
ATCCGACGCAGCACGACCGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
RSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKDFVIEGNDDYDFVICEIGGTVGDIEAMPFMEAIRQLG
NDLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRPSAVIQALDV
ANIYDVPIAYHKEGLDDEVLAAFGIEPAPKPRLDPWEEVCNRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALHHGGIANR
VKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKIHAARFARERKVPYFGICFGMQMAVIEAARNLADVSGA
SSTEFGPTKEPVVGLMTEWVKGNELQKRTAAGDLGGTMRLGAYKAALKKGTKISDIYGSTDISERHRHRYEVNVDYKDRL
ESCGLVFSGMSPDGVLPETIEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAATEQSRLV

Sequences:

>Translated_542_residues
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTG
RSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKDFVIEGNDDYDFVICEIGGTVGDIEAMPFMEAIRQLG
NDLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRPSAVIQALDV
ANIYDVPIAYHKEGLDDEVLAAFGIEPAPKPRLDPWEEVCNRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALHHGGIANR
VKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKIHAARFARERKVPYFGICFGMQMAVIEAARNLADVSGA
SSTEFGPTKEPVVGLMTEWVKGNELQKRTAAGDLGGTMRLGAYKAALKKGTKISDIYGSTDISERHRHRYEVNVDYKDRL
ESCGLVFSGMSPDGVLPETIEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAATEQSRLV
>Mature_541_residues
ARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVFVTDDGAETDLDLGHYERFTGR
SATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTNEIKDFVIEGNDDYDFVICEIGGTVGDIEAMPFMEAIRQLGN
DLPRGTAVYVHLTLMPYIPAAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRPSAVIQALDVA
NIYDVPIAYHKEGLDDEVLAAFGIEPAPKPRLDPWEEVCNRIRTPEGEVTIAIVGKYTGLKDAYKSLIEALHHGGIANRV
KVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSEGKIHAARFARERKVPYFGICFGMQMAVIEAARNLADVSGAS
STEFGPTKEPVVGLMTEWVKGNELQKRTAAGDLGGTMRLGAYKAALKKGTKISDIYGSTDISERHRHRYEVNVDYKDRLE
SCGLVFSGMSPDGVLPETIEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAATEQSRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=557, Percent_Identity=44.165170556553, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=563, Percent_Identity=43.6944937833037, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI28559083, Length=563, Percent_Identity=43.6944937833037, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI221316689, Length=563, Percent_Identity=43.6944937833037, Blast_Score=455, Evalue=1e-128,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=56.1567164179104, Blast_Score=589, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=36.7816091954023, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40.8771929824561, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=41.0934744268078, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=559, Percent_Identity=43.649373881932, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21357815, Length=505, Percent_Identity=42.970297029703, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59853; Mature: 59722

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EIKDFVIEGNDDYDFVICEIGGTVGDIEAMPFMEAIRQLGNDLPRGTAVYVHLTLMPYIP
HHHHEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
AAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRPSAVIQALDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEEEEEEECCHHHHHHHHHH
ANIYDVPIAYHKEGLDDEVLAAFGIEPAPKPRLDPWEEVCNRIRTPEGEVTIAIVGKYTG
CCEECCCHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALHHGGIANRVKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHHCEEEECCCCCCCCCC
GKIHAARFARERKVPYFGICFGMQMAVIEAARNLADVSGASSTEFGPTKEPVVGLMTEWV
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
KGNELQKRTAAGDLGGTMRLGAYKAALKKGTKISDIYGSTDISERHRHRYEVNVDYKDRL
CCCHHHHHHHCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCHHHHHCCEEEEECCHHHHH
ESCGLVFSGMSPDGVLPETIEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAATEQSR
HHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC
>Mature Secondary Structure 
ARYVFITGGVVSSLGKGIAAAALGALLQARGYRVRLRKLDPYLNVDPGTMSPTQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGRSATKTDNITTGRIYKNIIDKERRGDYLGATVQVIPHVTN
EECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EIKDFVIEGNDDYDFVICEIGGTVGDIEAMPFMEAIRQLGNDLPRGTAVYVHLTLMPYIP
HHHHEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCC
AAGELKTKPTQHSVKELQALGIHPDILLVRADREIPEAERRKLSLFCNVRPSAVIQALDV
CCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCEEEEEEECCHHHHHHHHHH
ANIYDVPIAYHKEGLDDEVLAAFGIEPAPKPRLDPWEEVCNRIRTPEGEVTIAIVGKYTG
CCEECCCHHHHCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCC
LKDAYKSLIEALHHGGIANRVKVKLEWIESEVFEKEDPAPYLEKVHGILVPGGFGERGSE
HHHHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHCCCCCCHHHHHHCEEEECCCCCCCCCC
GKIHAARFARERKVPYFGICFGMQMAVIEAARNLADVSGASSTEFGPTKEPVVGLMTEWV
CCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
KGNELQKRTAAGDLGGTMRLGAYKAALKKGTKISDIYGSTDISERHRHRYEVNVDYKDRL
CCCHHHHHHHCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCHHHHHCCEEEEECCHHHHH
ESCGLVFSGMSPDGVLPETIEYPDHPWFIGVQYHPELKSRPLDPHPLFASFIEAATEQSR
HHCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHCCCCCCCHHHHHHHHHHHHHHC
LV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]