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The map label for this gene is citZ [H]

Identifier: 209549423

GI number: 209549423

Start: 1877042

End: 1878187

Strand: Reverse

Name: citZ [H]

Synonym: Rleg2_1829

Alternate gene names: 209549423

Gene position: 1878187-1877042 (Counterclockwise)

Preceding gene: 209549425

Following gene: 209549422

Centisome position: 41.39

GC content: 67.8

Gene sequence:

>1146_bases
ATGCCGTGGCTGACCGCCGAGGAGGCGCTGCAGGCGCTGAAGACCAAGCCGCAGACGCTCTACGCCAATGTCAGCCGCGG
GCGCATCCGCGCCAAGCCCGATCCTGCCGACCCGCGACGCAGCCTCTACCAGGCTGCCGACGTGCAGCGGCTGGCCGAAC
GCCATGCCGGCCGGCGTAAAGCCGAAACGGTTGCCACCGAGACGATCCGTTGGGGCGACCCGGTGCTCTCCTCGGCGATC
TCCACCATCATCGGCGGACGGCTTTTCTATCGCGGACGGGATGCCGCCGATTTCGCCGACACGGCGACGCTCGAGCAAAC
GGCGGCATTGCTCTGGAACGGTGCGGAAGCGCTTGCTTCCCGCACTGCGGCGGGACATGCGGCTCCGTCGCTTCAGGCTG
CATTCCTGGCGCTGGCAGGGCGGGTGACATCGGATCTGCCGTCGCTCGGTCGCTCGCAGGCAGCCCTTCGCCGGGAAGCA
GGCGGAGTGCTCTGCACGGTCGCCGATGCGCTGGCGCCGGGGTCTTCGGATAGGCCGCTGCATCTTCGGCTTGCGGAAAG
CTGGCAGCGGCCGCATGCCGCCGATTGCCTGCGCCGGGCGCTGGTGTTGCTTGCCGATCACGAACTCAACGCCTCGACCT
TTGCGGCACGGGTGACGGCATCGGCGGGTACTACCCTCTCGGCCTCGGTGCTTTCCGGCCTGGCGACGCTGACCGGGCCG
CTGCATGGTGCGGCCTGGCAGGGCGTCGACGCGCTGATCGAGGCGGCTTCGGCTGTTGGCGCGGAGCAGGCGATTCGCCG
CACGCTTGCCCAGGGCCATCGCCTGTCGGCCTTTGGCCATCCGCTCTATCCGGATGGTGATATCAGGGCTCTGGCGTTGC
TTTCGCACTTTCCCCTGCCGCAGCCGTTTGCCGAGGTGCGGGAAACCGGCGAAGACATGGTCGGCGAGAAGGTTAACGTC
GATTTCGCGCTTGCGGCGATGGCCGCCGCCTTCGATCTGCCGCGGGAGGCGCCGATCATCATGTTCTCGCTTGCCCGTTC
CACCGGCTGGCTGGCGCATGCAATGGAGCAGATCGACAGCGGCGAATTGATCCGGCCGCGGGCCCGTTATACCGGGCCGT
CGCCCGAAACTACCATCAACCCTTAA

Upstream 100 bases:

>100_bases
TCCAAGCCATTTTTCATATTGTTTCTCCTTTACCGCCCGGATGATCCGGCCTAGTTTCATTGACGTCAATCTTGATGCTA
TTGATCAATATGAGGCTCCG

Downstream 100 bases:

>100_bases
GAATTGGTTAGGGAAATATCCGAATTCGGCTATCATGCCCGACATGGAAGAAGACGGTTGGCGGGCGCTTTCGAAAAGCT
GTTCGCACGGTCATGGGGGC

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MPWLTAEEALQALKTKPQTLYANVSRGRIRAKPDPADPRRSLYQAADVQRLAERHAGRRKAETVATETIRWGDPVLSSAI
STIIGGRLFYRGRDAADFADTATLEQTAALLWNGAEALASRTAAGHAAPSLQAAFLALAGRVTSDLPSLGRSQAALRREA
GGVLCTVADALAPGSSDRPLHLRLAESWQRPHAADCLRRALVLLADHELNASTFAARVTASAGTTLSASVLSGLATLTGP
LHGAAWQGVDALIEAASAVGAEQAIRRTLAQGHRLSAFGHPLYPDGDIRALALLSHFPLPQPFAEVRETGEDMVGEKVNV
DFALAAMAAAFDLPREAPIIMFSLARSTGWLAHAMEQIDSGELIRPRARYTGPSPETTINP

Sequences:

>Translated_381_residues
MPWLTAEEALQALKTKPQTLYANVSRGRIRAKPDPADPRRSLYQAADVQRLAERHAGRRKAETVATETIRWGDPVLSSAI
STIIGGRLFYRGRDAADFADTATLEQTAALLWNGAEALASRTAAGHAAPSLQAAFLALAGRVTSDLPSLGRSQAALRREA
GGVLCTVADALAPGSSDRPLHLRLAESWQRPHAADCLRRALVLLADHELNASTFAARVTASAGTTLSASVLSGLATLTGP
LHGAAWQGVDALIEAASAVGAEQAIRRTLAQGHRLSAFGHPLYPDGDIRALALLSHFPLPQPFAEVRETGEDMVGEKVNV
DFALAAMAAAFDLPREAPIIMFSLARSTGWLAHAMEQIDSGELIRPRARYTGPSPETTINP
>Mature_380_residues
PWLTAEEALQALKTKPQTLYANVSRGRIRAKPDPADPRRSLYQAADVQRLAERHAGRRKAETVATETIRWGDPVLSSAIS
TIIGGRLFYRGRDAADFADTATLEQTAALLWNGAEALASRTAAGHAAPSLQAAFLALAGRVTSDLPSLGRSQAALRREAG
GVLCTVADALAPGSSDRPLHLRLAESWQRPHAADCLRRALVLLADHELNASTFAARVTASAGTTLSASVLSGLATLTGPL
HGAAWQGVDALIEAASAVGAEQAIRRTLAQGHRLSAFGHPLYPDGDIRALALLSHFPLPQPFAEVRETGEDMVGEKVNVD
FALAAMAAAFDLPREAPIIMFSLARSTGWLAHAMEQIDSGELIRPRARYTGPSPETTINP

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=362, Percent_Identity=25.414364640884, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786939, Length=196, Percent_Identity=32.1428571428571, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40472; Mature: 40341

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWLTAEEALQALKTKPQTLYANVSRGRIRAKPDPADPRRSLYQAADVQRLAERHAGRRK
CCCCCHHHHHHHHHCCCHHEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHH
AETVATETIRWGDPVLSSAISTIIGGRLFYRGRDAADFADTATLEQTAALLWNGAEALAS
HHHHHHHHHHCCCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
RTAAGHAAPSLQAAFLALAGRVTSDLPSLGRSQAALRREAGGVLCTVADALAPGSSDRPL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCE
HLRLAESWQRPHAADCLRRALVLLADHELNASTFAARVTASAGTTLSASVLSGLATLTGP
EEEEHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LHGAAWQGVDALIEAASAVGAEQAIRRTLAQGHRLSAFGHPLYPDGDIRALALLSHFPLP
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
QPFAEVRETGEDMVGEKVNVDFALAAMAAAFDLPREAPIIMFSLARSTGWLAHAMEQIDS
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
GELIRPRARYTGPSPETTINP
CCEECCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PWLTAEEALQALKTKPQTLYANVSRGRIRAKPDPADPRRSLYQAADVQRLAERHAGRRK
CCCCHHHHHHHHHCCCHHEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHH
AETVATETIRWGDPVLSSAISTIIGGRLFYRGRDAADFADTATLEQTAALLWNGAEALAS
HHHHHHHHHHCCCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
RTAAGHAAPSLQAAFLALAGRVTSDLPSLGRSQAALRREAGGVLCTVADALAPGSSDRPL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCE
HLRLAESWQRPHAADCLRRALVLLADHELNASTFAARVTASAGTTLSASVLSGLATLTGP
EEEEHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
LHGAAWQGVDALIEAASAVGAEQAIRRTLAQGHRLSAFGHPLYPDGDIRALALLSHFPLP
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
QPFAEVRETGEDMVGEKVNVDFALAAMAAAFDLPREAPIIMFSLARSTGWLAHAMEQIDS
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCC
GELIRPRARYTGPSPETTINP
CCEECCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]