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The map label for this gene is glnA [H]

Identifier: 209549332

GI number: 209549332

Start: 1776636

End: 1778045

Strand: Reverse

Name: glnA [H]

Synonym: Rleg2_1736

Alternate gene names: 209549332

Gene position: 1778045-1776636 (Counterclockwise)

Preceding gene: 209549333

Following gene: 209549331

Centisome position: 39.18

GC content: 59.29

Gene sequence:

>1410_bases
ATGGCGACCGCAAGCGAAATTCTGAAGCAGATCAAGGAGAACGACGTAAAGTTCGTCGATCTGCGCTTCACCGACCCGAA
GGGCAAGCTGCAGCATGTGACGATGGACGTTGTCTGCGTCGACGAAGACATGTTCGCTGACGGCGTGATGTTCGACGGCT
CCTCGATCGGCGGCTGGAAGGCCATCAACGAGTCCGACATGGTGCTGATGCCCGATACCGAGACGGTGCATATGGACCCG
TTCTTCGCTCAGTCGACTATGGTCATCGTCTGCGATATTCTCGATCCGGTTTCCGGCGAAGCTTATAACCGCGATCCGCG
CGGCACCGCCAAGAAGGCCGAAGCCTATCTCAAGGCATCGGGCATCGGCGACACGATTTTCGTCGGCCCGGAAGCCGAAT
TCTTCGTCTTCGACGACGTCAAGTACAAGGCTGATCCCTACAATACCGGCTTCAAGCTCGATTCGACCGAACTGCCGTCG
AATGACGACACCGATTACGAAACCGGCAACCTCGGCCATCGCCCGCGTGTCAAGGGCGGCTATTTCCCGGTTCCGCCGGT
CGACAGCGCCCAGGACATGCGTTCGGAAATGCTGACGGTTCTCTCCGAAATGGGCGTCGTCGTCGAAAAGCATCACCATG
AAGTCGCCGCCGCCCAGCACGAACTCGGTATCAAGTTCGATACGCTGGTGCGCAACGCCGACAAGATGCAGATCTACAAA
TACGTCGTGCACCAGGTGGCCAATGCCTATGGCAAGACGGCGACCTTCATGCCGAAGCCGATCTTCGGCGACAACGGCTC
GGGCATGCACGTGCATCAGTCGATCTGGAAGGGCGGCAAGCCGACCTTCGCCGGCGACGAATATGCCGGTCTCTCCGAGA
GCTGCCTCTACTACATCGGCGGCATCATCAAGCATGCCAAGGCGATCAACGCCTTCACCAATCCGTCGACGAACTCCTAC
AAGCGTCTCGTCCCGGGCTATGAGGCACCGGTTCTGCTGGCTTACTCCGCCCGCAACCGCTCGGCCTCCTGCCGCATTCC
GTTCGGCACCAACCCGAAGGCAAAGCGCGTCGAAGTCCGTTTCCCGGATCCGACCGCCAACCCCTATCTCGCCTTCGCCG
CCATGCTGATGGCCGGCCTCGACGGCATCAAGAACAAGATCCATCCCGGCAAGGCCATGGACAAGGATCTCTACGACCTG
CCGCCGAAGGAATTGAAGAAGATCCCGACCGTCTGCGGCTCGCTGCGCGAAGCCCTTGAAAGCCTCGACAAGGACCGCAA
GTTCCTGACGGCCGGCGGCGTGTTCGACGACGACCAGATCGATGCCTTCATCGAACTGAAGATGGCAGAGGTGATGCGTT
TCGAAATGACGCCGCACCCTGTCGAATACGACATGTACTATTCGGCCTGA

Upstream 100 bases:

>100_bases
ACCGGCATCGACGCCATCTGACCCACTTTGCCAAGCGCGCAAAGTTCGATACGGTCAGCGCAGCTATGTATTGTCATCGT
AAATCGGAGGAAGGTATTTA

Downstream 100 bases:

>100_bases
TTGGCCTGAACACGGAAAGTCCCGGTCTGCCGGGGCTTTCCCCTCCCAGGGTCATGGACGCCAGCAGGAGGATATGACGT
TCATGTGACGCGCTTGCGCA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MATASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDTETVHMDP
FFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPS
NDDTDYETGNLGHRPRVKGGYFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAAQHELGIKFDTLVRNADKMQIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLYYIGGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGTNPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDL
PPKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYSA

Sequences:

>Translated_469_residues
MATASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDTETVHMDP
FFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPS
NDDTDYETGNLGHRPRVKGGYFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAAQHELGIKFDTLVRNADKMQIYK
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLYYIGGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPFGTNPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDL
PPKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYSA
>Mature_468_residues
ATASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWKAINESDMVLMPDTETVHMDPF
FAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKASGIGDTIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSN
DDTDYETGNLGHRPRVKGGYFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAAQHELGIKFDTLVRNADKMQIYKY
VVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLYYIGGIIKHAKAINAFTNPSTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPFGTNPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDLP
PKELKKIPTVCGSLREALESLDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYSA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=416, Percent_Identity=24.5192307692308, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=65.7387580299786, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=315, Percent_Identity=26.3492063492064, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52006; Mature: 51875

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWK
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCE
AINESDMVLMPDTETVHMDPFFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKAS
ECCCCCEEEECCCCEEEECCHHHCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
GIGDTIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAAQHELGIKFDTLVRNADKMQIYK
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGTNPKAKRVEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDLPPKELKKIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYSA
HHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECEEECC
>Mature Secondary Structure 
ATASEILKQIKENDVKFVDLRFTDPKGKLQHVTMDVVCVDEDMFADGVMFDGSSIGGWK
CCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEEECCCCCCCCEEECCCCCCCCE
AINESDMVLMPDTETVHMDPFFAQSTMVIVCDILDPVSGEAYNRDPRGTAKKAEAYLKAS
ECCCCCEEEECCCCEEEECCHHHCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
GIGDTIFVGPEAEFFVFDDVKYKADPYNTGFKLDSTELPSNDDTDYETGNLGHRPRVKGG
CCCCEEEECCCCCEEEEECCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVPPVDSAQDMRSEMLTVLSEMGVVVEKHHHEVAAAQHELGIKFDTLVRNADKMQIYK
CCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
YVVHQVANAYGKTATFMPKPIFGDNGSGMHVHQSIWKGGKPTFAGDEYAGLSESCLYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPFGTNPKAKRVEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCEEEEE
FPDPTANPYLAFAAMLMAGLDGIKNKIHPGKAMDKDLYDLPPKELKKIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
SLDKDRKFLTAGGVFDDDQIDAFIELKMAEVMRFEMTPHPVEYDMYYSA
HHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2882467 [H]