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The map label for this gene is reg1 [H]

Identifier: 209549294

GI number: 209549294

Start: 1727589

End: 1728644

Strand: Reverse

Name: reg1 [H]

Synonym: Rleg2_1697

Alternate gene names: 209549294

Gene position: 1728644-1727589 (Counterclockwise)

Preceding gene: 209549300

Following gene: 209549288

Centisome position: 38.09

GC content: 66.1

Gene sequence:

>1056_bases
ATGACAGCGGAGGAGAAAAAAGGCAGGCGCACGCGTCTTGACGACATCGCCGCCCGGTGCGGCGTCTCGATCAGCACGGT
CTCACGCGCCTTGGCCGGCGAAAAGGGCGTCCGGCCGGAGATTCGCAAGCTGGTGCTGGAAACGGCAAGTGCCGTCAGCT
ACGCCTTGCCGGCGAGCGTCGCCGGCAAGAAGGTCATGCTCGTCGCCTCGGGCGCTGCAATGATCGACTATGTCCGCAAC
CAGTTCACGCTCCATGTGCTCGAAGGCCTGAATGCCCGCGCGGCCGCCCTCGGCATCGAACTCGCCATGCGCCCGGTCGC
CGACAAAGCCGATGAAGCGCGCGTCGTCGCCGAGATGCGGGACGATCCGAGTTTCGGCGGCATGCTGATCCTGACCGTCG
ACGATGAGGATATGCTGGCGGCAGCCGCCGGTCTCGGAAAGCCCGTCGTGCTCGTCAACAGCGACGATCCCTATATGCGG
CTTTCGAGTGTGACGCCCTGCAACCGCTCGGCCGCCTTCATCGCCGCCGAGCACCTGATCAAGGCGGGGCATGAACGCGT
CCTGTTCATGCTGCGACCGGGACGTCGAACGATCGAGCGGCGCCTGGAGGGCTGGCGCGATGCCCTGCAGCATCACGGGC
TGCAGGCCGATGCCGATCTGGTGCTCGAAGTCGATGACTGGCTGCCGGAATTGGGCGCCGAGGCGATCACCCGCCTCGTC
CGCGACAAGGGCCTTTGCTTCACCGCCATCCTGACAGCGGGCGACAGCCTTGCCAACGGCGCCGTGCTCGGATTGCAGGC
GATGGGTTACGCCGTGCCGCAGGACATCTCCGTCATGGGCATCGACGACCTGCCGCAATCGGCCTTTCTCAATCCGCCGC
TTTCGACGGTGCATATCCCGATGCGCGAACTCGGCGCCACCGCGCTCGATCTGTTGCGCGACATGATGCTCGGCCTTGCG
ACGCCGCGGCGGCGTGTCGAGCTTTCCTGCCATCTCGTCGAAAGAGGCAGCGTCGCAGCTGTCAGCGGTGCAGATGCGGA
ACCCCGGGGGCGCTGA

Upstream 100 bases:

>100_bases
CCGCTTGGTCGCGGACTCCTCATGGGCAATTGTGCATAGGCATTCCGTTTGCGCAATCTGAAAGATTTTTGCATAAAGGC
ATAGGAGCGAGGAGAGCGAC

Downstream 100 bases:

>100_bases
GGCCACTGCTGTCCCGGTTGGAGATCGGCGAGAGCCGCCCCTCATCCGGCTGCCGCCACCTTCTCCCCGTAAACGGGGCG
AAGGGGATATGCCGCAAGCT

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTAEEKKGRRTRLDDIAARCGVSISTVSRALAGEKGVRPEIRKLVLETASAVSYALPASVAGKKVMLVASGAAMIDYVRN
QFTLHVLEGLNARAAALGIELAMRPVADKADEARVVAEMRDDPSFGGMLILTVDDEDMLAAAAGLGKPVVLVNSDDPYMR
LSSVTPCNRSAAFIAAEHLIKAGHERVLFMLRPGRRTIERRLEGWRDALQHHGLQADADLVLEVDDWLPELGAEAITRLV
RDKGLCFTAILTAGDSLANGAVLGLQAMGYAVPQDISVMGIDDLPQSAFLNPPLSTVHIPMRELGATALDLLRDMMLGLA
TPRRRVELSCHLVERGSVAAVSGADAEPRGR

Sequences:

>Translated_351_residues
MTAEEKKGRRTRLDDIAARCGVSISTVSRALAGEKGVRPEIRKLVLETASAVSYALPASVAGKKVMLVASGAAMIDYVRN
QFTLHVLEGLNARAAALGIELAMRPVADKADEARVVAEMRDDPSFGGMLILTVDDEDMLAAAAGLGKPVVLVNSDDPYMR
LSSVTPCNRSAAFIAAEHLIKAGHERVLFMLRPGRRTIERRLEGWRDALQHHGLQADADLVLEVDDWLPELGAEAITRLV
RDKGLCFTAILTAGDSLANGAVLGLQAMGYAVPQDISVMGIDDLPQSAFLNPPLSTVHIPMRELGATALDLLRDMMLGLA
TPRRRVELSCHLVERGSVAAVSGADAEPRGR
>Mature_350_residues
TAEEKKGRRTRLDDIAARCGVSISTVSRALAGEKGVRPEIRKLVLETASAVSYALPASVAGKKVMLVASGAAMIDYVRNQ
FTLHVLEGLNARAAALGIELAMRPVADKADEARVVAEMRDDPSFGGMLILTVDDEDMLAAAAGLGKPVVLVNSDDPYMRL
SSVTPCNRSAAFIAAEHLIKAGHERVLFMLRPGRRTIERRLEGWRDALQHHGLQADADLVLEVDDWLPELGAEAITRLVR
DKGLCFTAILTAGDSLANGAVLGLQAMGYAVPQDISVMGIDDLPQSAFLNPPLSTVHIPMRELGATALDLLRDMMLGLAT
PRRRVELSCHLVERGSVAAVSGADAEPRGR

Specific function: Transcription repressor involved in control of expression of alpha-amylase and chitinase genes and of actinorhodin production [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=348, Percent_Identity=27.2988505747126, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789068, Length=344, Percent_Identity=28.4883720930233, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=24.1691842900302, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786540, Length=345, Percent_Identity=27.536231884058, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=27.8787878787879, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=24.0963855421687, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788474, Length=341, Percent_Identity=24.9266862170088, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789202, Length=156, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789456, Length=320, Percent_Identity=24.375, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37614; Mature: 37483

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEEKKGRRTRLDDIAARCGVSISTVSRALAGEKGVRPEIRKLVLETASAVSYALPASV
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHC
AGKKVMLVASGAAMIDYVRNQFTLHVLEGLNARAAALGIELAMRPVADKADEARVVAEMR
CCCEEEEEECCHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DDPSFGGMLILTVDDEDMLAAAAGLGKPVVLVNSDDPYMRLSSVTPCNRSAAFIAAEHLI
CCCCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHH
KAGHERVLFMLRPGRRTIERRLEGWRDALQHHGLQADADLVLEVDDWLPELGAEAITRLV
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
RDKGLCFTAILTAGDSLANGAVLGLQAMGYAVPQDISVMGIDDLPQSAFLNPPLSTVHIP
HHCCCEEEEEECCCCCCCCCCEEHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCEEECC
MRELGATALDLLRDMMLGLATPRRRVELSCHLVERGSVAAVSGADAEPRGR
HHHHCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure 
TAEEKKGRRTRLDDIAARCGVSISTVSRALAGEKGVRPEIRKLVLETASAVSYALPASV
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHC
AGKKVMLVASGAAMIDYVRNQFTLHVLEGLNARAAALGIELAMRPVADKADEARVVAEMR
CCCEEEEEECCHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DDPSFGGMLILTVDDEDMLAAAAGLGKPVVLVNSDDPYMRLSSVTPCNRSAAFIAAEHLI
CCCCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHHHHHH
KAGHERVLFMLRPGRRTIERRLEGWRDALQHHGLQADADLVLEVDDWLPELGAEAITRLV
HCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
RDKGLCFTAILTAGDSLANGAVLGLQAMGYAVPQDISVMGIDDLPQSAFLNPPLSTVHIP
HHCCCEEEEEECCCCCCCCCCEEHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCEEECC
MRELGATALDLLRDMMLGLATPRRRVELSCHLVERGSVAAVSGADAEPRGR
HHHHCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9335287 [H]