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The map label for this gene is yhjG [H]

Identifier: 209549255

GI number: 209549255

Start: 1684694

End: 1686220

Strand: Reverse

Name: yhjG [H]

Synonym: Rleg2_1656

Alternate gene names: 209549255

Gene position: 1686220-1684694 (Counterclockwise)

Preceding gene: 209549257

Following gene: 209549251

Centisome position: 37.16

GC content: 66.8

Gene sequence:

>1527_bases
ATGTCACAGAATTCCACAGTCGACGTGCTGATATCCGGCGCCGGTGCAGCCGGGCTGACGCTGGCGATCGAGCTGGCGCG
GCGCGGCGTCAGCTTCCGCCTGATCGAAAAATTGAACGACCCCTTCCGCGGCTCCCGCGGCAAGGGCATCCAGCCCCGCA
GCCAGGAAGTCTTCGAAGATCTCGGCATCCTCGACCGGATCGTTTCGCTCGGCGGTGCCTATCCCCGGCAGCGGGACTAT
CGCGACGATGGAAGCGTCGGCGAATCCGAGGTCGTGCTGCATGAGCCGCCGACGCCTGCCGAACCCTACCATCTGCCGCT
GATGGTGCCGCAGGTCCTGACCGAGGGCGTGATGCGCGAGCGCCTGCTCGAGCTCGGGCACCACCCGGAATTCGGATGTG
AACTCATCGGCTTCGAACAGGACGATGCCGGGGTAACGGCGCGGCTCAACGGCCAGTCCGGCGAAGAGACCATCCGCGTG
CGCTGGCTTGTCGGCGCCGATGGTGGGCGCAGCTTCGTGCGCCACGCGCTCGATATCGGCTTTCCCGGCAAGACGCTCGG
CGTGCGCGCCATCGTCGCCGATGTCGTGCTGACGGGGCTGGATCGCGACGCCTGGCATCGTTTCGGCGAAGGCGACATGC
AGCGGCAGATTGCGCTTTGCCCGCTCGCCGGAACGGAGTTGTTCCAGATCCAGGGACCGATCCCGCTCGAAGGCGAGATC
GACCTGTCGGCGGCCGGCTTGACCGCGCTGGTGGGCGAGCGCACCGGCCGCGACGATATTCACGTCCGATCGGTCTCCTG
GGCCTCGGCCTTCAACATGAACGCCCGGCTTGCCGATCGCTACCGGCTGGGCCGCGTATTCCTGGCCGGCGATGCCGCCC
ATACGCATCCGCCGACCGGCGGCCAGGGTCTCAATACCAGCGTGCAGGACGCCTATAATCTCGGATGGAAACTGGCGGCA
GTCGCTGCCGGCGCGCCGGATGCGCTGCTCGACAGCTATGAGGAAGAGCGCCGCCCGATCGCAGCGGCGATGCTGGGCCT
TGCCACTAATCTTCTCGATGCGCTGAAGCGCGGCGATCTCAGGCGCGGCCGCGAGGTCCATCAGCTCGATATCGGCTATT
CCGAGTCTTCTCTCGCGCTGGAAAAGCCGGAGCGGCGCGGCGGCCTGCTGGCCGGCGACCGTGCGCCCGATGCACCGCTC
ATTGGTGCGGCCGGCCAGCCGCGGCGTCTGTTCGAACTGTTCAAGGGCACGCACTGGACGTTACTTGGCTACGAGATCGA
ACAGGCAGCCGTACCGGCGCGCCGCGGATTGCACATCCACAGCATCGGCCGGCGCGGCGATCTCATCGACCGAGGCGGAC
ACTTCCTTGACGCCTACACCCTGACGCCGGGCGACTTCGTGCTGGTTCGTCCAGACGGTTATATCGGCGCCATCGTCGCG
TCCGGCGATAGTTGGGCGCTGAAAGCCTATCTCGCCAAGGTCGGCCTTGGCCGGGGGCAAGAGAGCCTTCATGCGGCGAC
ACGCTGA

Upstream 100 bases:

>100_bases
AGTTCACGCAGTTTTTCGGATATATGTGTATTTTGCACGCTTTTTCTTGACGGCATGTCGATTCTCCTATTATGTGCATA
ATACACATATGGAGATGACA

Downstream 100 bases:

>100_bases
TCGGCGCATTCCCCACTCATAATCGCTCATTCCGAAACAACCACCCTGCTGTGATCGCCTCGACCGCCTTTCATTGTGGG
TCGACCGTGGCTAGGCGGAG

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSQNSTVDVLISGAGAAGLTLAIELARRGVSFRLIEKLNDPFRGSRGKGIQPRSQEVFEDLGILDRIVSLGGAYPRQRDY
RDDGSVGESEVVLHEPPTPAEPYHLPLMVPQVLTEGVMRERLLELGHHPEFGCELIGFEQDDAGVTARLNGQSGEETIRV
RWLVGADGGRSFVRHALDIGFPGKTLGVRAIVADVVLTGLDRDAWHRFGEGDMQRQIALCPLAGTELFQIQGPIPLEGEI
DLSAAGLTALVGERTGRDDIHVRSVSWASAFNMNARLADRYRLGRVFLAGDAAHTHPPTGGQGLNTSVQDAYNLGWKLAA
VAAGAPDALLDSYEEERRPIAAAMLGLATNLLDALKRGDLRRGREVHQLDIGYSESSLALEKPERRGGLLAGDRAPDAPL
IGAAGQPRRLFELFKGTHWTLLGYEIEQAAVPARRGLHIHSIGRRGDLIDRGGHFLDAYTLTPGDFVLVRPDGYIGAIVA
SGDSWALKAYLAKVGLGRGQESLHAATR

Sequences:

>Translated_508_residues
MSQNSTVDVLISGAGAAGLTLAIELARRGVSFRLIEKLNDPFRGSRGKGIQPRSQEVFEDLGILDRIVSLGGAYPRQRDY
RDDGSVGESEVVLHEPPTPAEPYHLPLMVPQVLTEGVMRERLLELGHHPEFGCELIGFEQDDAGVTARLNGQSGEETIRV
RWLVGADGGRSFVRHALDIGFPGKTLGVRAIVADVVLTGLDRDAWHRFGEGDMQRQIALCPLAGTELFQIQGPIPLEGEI
DLSAAGLTALVGERTGRDDIHVRSVSWASAFNMNARLADRYRLGRVFLAGDAAHTHPPTGGQGLNTSVQDAYNLGWKLAA
VAAGAPDALLDSYEEERRPIAAAMLGLATNLLDALKRGDLRRGREVHQLDIGYSESSLALEKPERRGGLLAGDRAPDAPL
IGAAGQPRRLFELFKGTHWTLLGYEIEQAAVPARRGLHIHSIGRRGDLIDRGGHFLDAYTLTPGDFVLVRPDGYIGAIVA
SGDSWALKAYLAKVGLGRGQESLHAATR
>Mature_507_residues
SQNSTVDVLISGAGAAGLTLAIELARRGVSFRLIEKLNDPFRGSRGKGIQPRSQEVFEDLGILDRIVSLGGAYPRQRDYR
DDGSVGESEVVLHEPPTPAEPYHLPLMVPQVLTEGVMRERLLELGHHPEFGCELIGFEQDDAGVTARLNGQSGEETIRVR
WLVGADGGRSFVRHALDIGFPGKTLGVRAIVADVVLTGLDRDAWHRFGEGDMQRQIALCPLAGTELFQIQGPIPLEGEID
LSAAGLTALVGERTGRDDIHVRSVSWASAFNMNARLADRYRLGRVFLAGDAAHTHPPTGGQGLNTSVQDAYNLGWKLAAV
AAGAPDALLDSYEEERRPIAAAMLGLATNLLDALKRGDLRRGREVHQLDIGYSESSLALEKPERRGGLLAGDRAPDAPLI
GAAGQPRRLFELFKGTHWTLLGYEIEQAAVPARRGLHIHSIGRRGDLIDRGGHFLDAYTLTPGDFVLVRPDGYIGAIVAS
GDSWALKAYLAKVGLGRGQESLHAATR

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=374, Percent_Identity=33.6898395721925, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54838; Mature: 54707

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNSTVDVLISGAGAAGLTLAIELARRGVSFRLIEKLNDPFRGSRGKGIQPRSQEVFED
CCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCCCCHHHHHHHH
LGILDRIVSLGGAYPRQRDYRDDGSVGESEVVLHEPPTPAEPYHLPLMVPQVLTEGVMRE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCEEHHHHHHHHHHHH
RLLELGHHPEFGCELIGFEQDDAGVTARLNGQSGEETIRVRWLVGADGGRSFVRHALDIG
HHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHCC
FPGKTLGVRAIVADVVLTGLDRDAWHRFGEGDMQRQIALCPLAGTELFQIQGPIPLEGEI
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHCEEEEEECCCCEEEEEECCCCCCCCC
DLSAAGLTALVGERTGRDDIHVRSVSWASAFNMNARLADRYRLGRVFLAGDAAHTHPPTG
CCCCCCHHHHHCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHEECEEEEECCCCCCCCCCC
GQGLNTSVQDAYNLGWKLAAVAAGAPDALLDSYEEERRPIAAAMLGLATNLLDALKRGDL
CCCCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCH
RRGREVHQLDIGYSESSLALEKPERRGGLLAGDRAPDAPLIGAAGQPRRLFELFKGTHWT
HCCCCEEEEECCCCCCCCEECCCHHCCCEEECCCCCCCCEECCCCCHHHHHHHHCCCCEE
LLGYEIEQAAVPARRGLHIHSIGRRGDLIDRGGHFLDAYTLTPGDFVLVRPDGYIGAIVA
EEEEEHHHHHCHHHCCEEEEECCCCCCCHHCCCCEEEEEEECCCCEEEECCCCEEEEEEE
SGDSWALKAYLAKVGLGRGQESLHAATR
CCCCHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
SQNSTVDVLISGAGAAGLTLAIELARRGVSFRLIEKLNDPFRGSRGKGIQPRSQEVFED
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCCCCHHHHHHHH
LGILDRIVSLGGAYPRQRDYRDDGSVGESEVVLHEPPTPAEPYHLPLMVPQVLTEGVMRE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECCEEHHHHHHHHHHHH
RLLELGHHPEFGCELIGFEQDDAGVTARLNGQSGEETIRVRWLVGADGGRSFVRHALDIG
HHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHCC
FPGKTLGVRAIVADVVLTGLDRDAWHRFGEGDMQRQIALCPLAGTELFQIQGPIPLEGEI
CCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHCEEEEEECCCCEEEEEECCCCCCCCC
DLSAAGLTALVGERTGRDDIHVRSVSWASAFNMNARLADRYRLGRVFLAGDAAHTHPPTG
CCCCCCHHHHHCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHEECEEEEECCCCCCCCCCC
GQGLNTSVQDAYNLGWKLAAVAAGAPDALLDSYEEERRPIAAAMLGLATNLLDALKRGDL
CCCCCCCHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCH
RRGREVHQLDIGYSESSLALEKPERRGGLLAGDRAPDAPLIGAAGQPRRLFELFKGTHWT
HCCCCEEEEECCCCCCCCEECCCHHCCCEEECCCCCCCCEECCCCCHHHHHHHHCCCCEE
LLGYEIEQAAVPARRGLHIHSIGRRGDLIDRGGHFLDAYTLTPGDFVLVRPDGYIGAIVA
EEEEEHHHHHCHHHCCEEEEECCCCCCCHHCCCCEEEEEEECCCCEEEECCCCEEEEEEE
SGDSWALKAYLAKVGLGRGQESLHAATR
CCCCHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]