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The map label for this gene is gltA [H]

Identifier: 209549195

GI number: 209549195

Start: 1621453

End: 1622742

Strand: Reverse

Name: gltA [H]

Synonym: Rleg2_1596

Alternate gene names: 209549195

Gene position: 1622742-1621453 (Counterclockwise)

Preceding gene: 209549197

Following gene: 209549176

Centisome position: 35.76

GC content: 59.84

Gene sequence:

>1290_bases
ATGACGGATCAAAGCGCTACAATAAAAATCGGTGACAAATCTGTCGACCTAGCCGTCAAGAGCGGCACGATCGGCCCGAG
CGTCATCGATATCGGTGCCCTCTACAAGAACACGGCTTCCTTCACCTACGATCCGGGTTTCACCTCGACCGCATCCTGTG
AATCGAAAATCACCTACATCGACGGCGACGAAGGTGTGCTGCTGCACCGCGGCTATCCGATCGAGCAGCTGGCCGAGCAT
GGCGATTTCCTCGAAGTCTGCTACCTGCTTCTCTACGGTGAACTGCCGACCACCGCACAGAAGAAGGATTTCGATTATCG
CGTCACGCACCACACCATGGTGCACGAGCAGATGAGCCGCTTCTTCACCGGCTTCCGCCGCGATGCGCATCCGATGGCCG
TCATGTGCGGCTGCGTCGGCGCGCTGTCGGCTTTCTATCACGACTCCACCGACATCACCGATCCGCACCAGCGCATGGTC
GCCAGCCTGCGTATGATCGCCAAGATGCCGACGCTTGCCGCCATGGCCTACAAGTACCATATCGGCCAGCCCTTCGTTTA
TCCGAAGAACGATCTCGACTACGCCTCGAACTTCCTGCGCATGTGCTTTGCCGTGCCTTGCGAAGAATATGTGGTCAATC
CGGTGCTTGCCCGGGCCATGGACCGCATCTTCATCCTGCATGCCGATCACGAGCAGAACGCCTCGACCTCGACCGTCCGT
CTCGCCGGTTCGTCGGGCGCCAACCCGTTTGCCTGCATCGCAGCCGGCATCGCCTGCCTCTGGGGCCCCGCCCATGGCGG
CGCGAACGAAGCAGCGCTCAACATGCTGCATGAGATCGGCTCCGTCGATCACATTCCGGAATATATCGCCCGCGCCAAGG
ACAAGAACGATCCGTTCCGCCTGATGGGCTTCGGTCACCGCGTCTACAAGAACTACGATCCGCGCGCCAAGATCATGCAG
AAGACGACGCATGAAGTGCTCGGCGAGCTCGGCATCAAGGACGATCCGTTGCTCGAAGTGGCGATGGAGCTGGAGCGCAT
CGCGCTCACCGACGAATATTTCATCGAAAAGAAGCTCTATCCGAACATCGACTTCTATTCCGGCATCACGCTGAAGGCGC
TGGGCTTCCCCACGACCATGTTCACCGTGCTCTTCGCGCTTGCCCGCACCGTCGGCTGGATCGCCCAGTGGAACGAGATG
ATCGAGGATCCGGAGCAGCGCATCGGCCGTCCGCGCCAGCTCTATATCGGCGAACCGAAGCGCGACTACATCCCGGTTTC
GAAGCGCTGA

Upstream 100 bases:

>100_bases
GCGGTAACTTGGCATTAGCTTCGCGATCATATAGCTATCGGTGAGGACGCTTAACCCCTATGGCGCTTTTATGGCGCCAC
CGCCATTTCGGAGGATCAGC

Downstream 100 bases:

>100_bases
TCGCCTCGGCCAATATATCGGCTAGAGAAGAAGAAAACCCGGTCAGAGACGACCGGGTTTTTTCTTGTTCAGCACCTCGA
GCAGGATTTCGGCGGCATGT

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTDQSATIKIGDKSVDLAVKSGTIGPSVIDIGALYKNTASFTYDPGFTSTASCESKITYIDGDEGVLLHRGYPIEQLAEH
GDFLEVCYLLLYGELPTTAQKKDFDYRVTHHTMVHEQMSRFFTGFRRDAHPMAVMCGCVGALSAFYHDSTDITDPHQRMV
ASLRMIAKMPTLAAMAYKYHIGQPFVYPKNDLDYASNFLRMCFAVPCEEYVVNPVLARAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLHEIGSVDHIPEYIARAKDKNDPFRLMGFGHRVYKNYDPRAKIMQ
KTTHEVLGELGIKDDPLLEVAMELERIALTDEYFIEKKLYPNIDFYSGITLKALGFPTTMFTVLFALARTVGWIAQWNEM
IEDPEQRIGRPRQLYIGEPKRDYIPVSKR

Sequences:

>Translated_429_residues
MTDQSATIKIGDKSVDLAVKSGTIGPSVIDIGALYKNTASFTYDPGFTSTASCESKITYIDGDEGVLLHRGYPIEQLAEH
GDFLEVCYLLLYGELPTTAQKKDFDYRVTHHTMVHEQMSRFFTGFRRDAHPMAVMCGCVGALSAFYHDSTDITDPHQRMV
ASLRMIAKMPTLAAMAYKYHIGQPFVYPKNDLDYASNFLRMCFAVPCEEYVVNPVLARAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLHEIGSVDHIPEYIARAKDKNDPFRLMGFGHRVYKNYDPRAKIMQ
KTTHEVLGELGIKDDPLLEVAMELERIALTDEYFIEKKLYPNIDFYSGITLKALGFPTTMFTVLFALARTVGWIAQWNEM
IEDPEQRIGRPRQLYIGEPKRDYIPVSKR
>Mature_428_residues
TDQSATIKIGDKSVDLAVKSGTIGPSVIDIGALYKNTASFTYDPGFTSTASCESKITYIDGDEGVLLHRGYPIEQLAEHG
DFLEVCYLLLYGELPTTAQKKDFDYRVTHHTMVHEQMSRFFTGFRRDAHPMAVMCGCVGALSAFYHDSTDITDPHQRMVA
SLRMIAKMPTLAAMAYKYHIGQPFVYPKNDLDYASNFLRMCFAVPCEEYVVNPVLARAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLHEIGSVDHIPEYIARAKDKNDPFRLMGFGHRVYKNYDPRAKIMQK
TTHEVLGELGIKDDPLLEVAMELERIALTDEYFIEKKLYPNIDFYSGITLKALGFPTTMFTVLFALARTVGWIAQWNEMI
EDPEQRIGRPRQLYIGEPKRDYIPVSKR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=384, Percent_Identity=26.0416666666667, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786939, Length=430, Percent_Identity=69.5348837209302, Blast_Score=626, Evalue=0.0,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=29.7297297297297, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17555174, Length=400, Percent_Identity=26, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=332, Percent_Identity=28.0120481927711, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=338, Percent_Identity=25.7396449704142, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=452, Percent_Identity=24.3362831858407, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640126, Length=430, Percent_Identity=26.2790697674419, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24640124, Length=430, Percent_Identity=26.2790697674419, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21356863, Length=348, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48064; Mature: 47932

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQSATIKIGDKSVDLAVKSGTIGPSVIDIGALYKNTASFTYDPGFTSTASCESKITYI
CCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEE
DGDEGVLLHRGYPIEQLAEHGDFLEVCYLLLYGELPTTAQKKDFDYRVTHHTMVHEQMSR
ECCCCEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHH
FFTGFRRDAHPMAVMCGCVGALSAFYHDSTDITDPHQRMVASLRMIAKMPTLAAMAYKYH
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHC
IGQPFVYPKNDLDYASNFLRMCFAVPCEEYVVNPVLARAMDRIFILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLHEIGSVDHIPEYIARAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNYDPRAKIMQKTTHEVLGELGIKDDPLLEVAMELERIALTDEYFIEKKLY
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNIDFYSGITLKALGFPTTMFTVLFALARTVGWIAQWNEMIEDPEQRIGRPRQLYIGEPK
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCC
RDYIPVSKR
CCCCCCCCC
>Mature Secondary Structure 
TDQSATIKIGDKSVDLAVKSGTIGPSVIDIGALYKNTASFTYDPGFTSTASCESKITYI
CCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEE
DGDEGVLLHRGYPIEQLAEHGDFLEVCYLLLYGELPTTAQKKDFDYRVTHHTMVHEQMSR
ECCCCEEEECCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHH
FFTGFRRDAHPMAVMCGCVGALSAFYHDSTDITDPHQRMVASLRMIAKMPTLAAMAYKYH
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHC
IGQPFVYPKNDLDYASNFLRMCFAVPCEEYVVNPVLARAMDRIFILHADHEQNASTSTVR
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLHEIGSVDHIPEYIARAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEE
LMGFGHRVYKNYDPRAKIMQKTTHEVLGELGIKDDPLLEVAMELERIALTDEYFIEKKLY
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PNIDFYSGITLKALGFPTTMFTVLFALARTVGWIAQWNEMIEDPEQRIGRPRQLYIGEPK
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCC
RDYIPVSKR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]