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The map label for this gene is gatA [H]

Identifier: 209549121

GI number: 209549121

Start: 1549444

End: 1550925

Strand: Direct

Name: gatA [H]

Synonym: Rleg2_1521

Alternate gene names: 209549121

Gene position: 1549444-1550925 (Clockwise)

Preceding gene: 209549120

Following gene: 209549122

Centisome position: 34.14

GC content: 63.16

Gene sequence:

>1482_bases
ATGAGCGAACTCACCAGCCTGACCATTGCCGAAGCCCGCCGGAAGCTGCGCGCCAAGGAAATCACCGCACTTGAGCTGAC
CGAGGCCTATATCTCGGCGATCGATGCGGCAAATGGTCATCTGAATGCCTATATCAAGGTGACGCCGGATCTCGCCCGCC
TGATGGCGAAGAATTCCGACGAGCGTATCGCTGCCGGCAAGGCCGGTGATCTCGAAGGCATTCCGCTCGGCATCAAGGAT
CTTTTTGCCACCGTCGGTGTGCACACACAGGCCTGCAGCCACATTCTCGACGGTTTCGAGCCGCGTTATGAATCAACTGT
CACGCAGAACCTCTGGGATGACGGCGCCGTCATGCTCGGCAAGCTGAACATGGATGAATTCGCCATGGGCTCGTCCAACG
AGACCTCGCACTACGGTGCGGTGATCAATCCCTGGCGTGCGGCAGGGTCCAACCAGCAGCTCGTTCCCGGCGGTTCCTCC
GGCGGCTCGGCCGCAGCTGTTGCCGCGCATCTTTGCGCCGGCGCCACCGCAACCGATACCGGCGGCTCGATCCGCCAGCC
GGCCGCCTTCACCGGCACCGTCGGCATCAAGCCGACCTATGGACGCTGCTCGCGCTGGGGCACTGTCGCCTTCGCCTCCT
CACTCGACCAGGCAGGCCCGATCGCCCGCGACGTGCGTGATGCCGCAATCCTTCTGAAGTCGATGGCAAGCGTCGACGCC
AAGGACACGACCTCGGTCGATCTGCCCGTGCCGGATTATGAGGCAGCGCTTGGCCAGTCGCTGAAGGGCATGAAGATCGG
CATTCCAAGCGAATACCGTGTCGATGGCATGCCTGAGGAGATCGAGACCCTCTGGCGCCAGGGCATCGCTTGGCTGAAGG
ATGCCGGCGCCGAAATCGTCGACATCTCGCTGCCGCACACCAAATACGCTCTTCCGGCCTATTACATCGTCGCTCCAGCC
GAGGCATCCTCGAACCTGGCGCGTTACGACGGCGTGCGCTACGGCCTGCGCGTCGACGGCAAGGATATCGTCGACATGTA
CGAGAAGACGCGCGCCGCCGGCTTCGGCAAGGAAGTCAAGCGCCGCATCATGATCGGCACCTATGTGCTGTCGGCCGGTT
ATTACGATGCCTATTACATCCGCGCCCAGAAGGTCCGGACGCTGATCAAGCGCGATTTCGAACTCGCCTTCAACGCCGGC
GTCGACGCCATCCTGACGCCGGCGACCCCGTCTTCCGCCTTCGGCGTCGCCGATGAGAACCTCGCTTCCGACCCGGTGAA
GATGTATCTGAACGACATCTTCACGGTCACGGTCAACATGGCGGGCCTGCCTGGCATCGCCGTGCCCGCCGGCCTCGATC
AGAAGGGCCTGCCCCTCGGCCTGCAGCTGATCGGCAAGCCCTTCGACGAGGAAACCCTCTTCAAGACGGCTCACGTGATC
GAGCAGGCAGCCGGCCGGTTCACGCCGGCCAGGTGGTGGTAA

Upstream 100 bases:

>100_bases
TCACCGATCACAATTTTTTCCTGGTGCCGAAAGTCGTCGAATAGGGCTTCAAGCCTCGTTCCGACCCTGTTGCCATTTTC
AGATCTGAAGCGAAAACATC

Downstream 100 bases:

>100_bases
CGGATCTAACGATCCGAAAGTTTGGGGCCGCCAACCACGAAGCGGTCGGCGCCATCGCCTTCGCCGCATGGGCCGCAGGC
GAGGCTTTCGAGGATTGCTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSELTSLTIAEARRKLRAKEITALELTEAYISAIDAANGHLNAYIKVTPDLARLMAKNSDERIAAGKAGDLEGIPLGIKD
LFATVGVHTQACSHILDGFEPRYESTVTQNLWDDGAVMLGKLNMDEFAMGSSNETSHYGAVINPWRAAGSNQQLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDA
KDTTSVDLPVPDYEAALGQSLKGMKIGIPSEYRVDGMPEEIETLWRQGIAWLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFNAG
VDAILTPATPSSAFGVADENLASDPVKMYLNDIFTVTVNMAGLPGIAVPAGLDQKGLPLGLQLIGKPFDEETLFKTAHVI
EQAAGRFTPARWW

Sequences:

>Translated_493_residues
MSELTSLTIAEARRKLRAKEITALELTEAYISAIDAANGHLNAYIKVTPDLARLMAKNSDERIAAGKAGDLEGIPLGIKD
LFATVGVHTQACSHILDGFEPRYESTVTQNLWDDGAVMLGKLNMDEFAMGSSNETSHYGAVINPWRAAGSNQQLVPGGSS
GGSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDA
KDTTSVDLPVPDYEAALGQSLKGMKIGIPSEYRVDGMPEEIETLWRQGIAWLKDAGAEIVDISLPHTKYALPAYYIVAPA
EASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFNAG
VDAILTPATPSSAFGVADENLASDPVKMYLNDIFTVTVNMAGLPGIAVPAGLDQKGLPLGLQLIGKPFDEETLFKTAHVI
EQAAGRFTPARWW
>Mature_492_residues
SELTSLTIAEARRKLRAKEITALELTEAYISAIDAANGHLNAYIKVTPDLARLMAKNSDERIAAGKAGDLEGIPLGIKDL
FATVGVHTQACSHILDGFEPRYESTVTQNLWDDGAVMLGKLNMDEFAMGSSNETSHYGAVINPWRAAGSNQQLVPGGSSG
GSAAAVAAHLCAGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDAK
DTTSVDLPVPDYEAALGQSLKGMKIGIPSEYRVDGMPEEIETLWRQGIAWLKDAGAEIVDISLPHTKYALPAYYIVAPAE
ASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGKEVKRRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFNAGV
DAILTPATPSSAFGVADENLASDPVKMYLNDIFTVTVNMAGLPGIAVPAGLDQKGLPLGLQLIGKPFDEETLFKTAHVIE
QAAGRFTPARWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=40.4858299595142, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=25.3937007874016, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=37.6975169300226, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=515, Percent_Identity=25.4368932038835, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17538252, Length=540, Percent_Identity=22.5925925925926, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=326, Percent_Identity=26.0736196319018, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=425, Percent_Identity=35.2941176470588, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=411, Percent_Identity=28.7104622871046, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320448, Length=247, Percent_Identity=28.3400809716599, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=39.2461197339246, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=24.2544731610338, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=517, Percent_Identity=25.3384912959381, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=25.4980079681275, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=25.4980079681275, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=25.4980079681275, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=25.4980079681275, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356731, Length=524, Percent_Identity=24.0458015267176, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550774, Length=262, Percent_Identity=27.8625954198473, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648435, Length=262, Percent_Identity=27.8625954198473, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648437, Length=262, Percent_Identity=27.8625954198473, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52748; Mature: 52617

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTSLTIAEARRKLRAKEITALELTEAYISAIDAANGHLNAYIKVTPDLARLMAKNSD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCC
ERIAAGKAGDLEGIPLGIKDLFATVGVHTQACSHILDGFEPRYESTVTQNLWDDGAVMLG
CEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSSNETSHYGAVINPWRAAGSNQQLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCCCCCEECCHHCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDA
CCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEAALGQSLKGMKIGIPSEYRVDGMPEEIETLWRQGIAWLKDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFNAGVDAILTPATPSSAFGVADEN
HHHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
LASDPVKMYLNDIFTVTVNMAGLPGIAVPAGLDQKGLPLGLQLIGKPFDEETLFKTAHVI
CCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
EQAAGRFTPARWW
HHHHCCCCCCCCC
>Mature Secondary Structure 
SELTSLTIAEARRKLRAKEITALELTEAYISAIDAANGHLNAYIKVTPDLARLMAKNSD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCC
ERIAAGKAGDLEGIPLGIKDLFATVGVHTQACSHILDGFEPRYESTVTQNLWDDGAVMLG
CEEECCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEE
KLNMDEFAMGSSNETSHYGAVINPWRAAGSNQQLVPGGSSGGSAAAVAAHLCAGATATDT
ECCHHHHCCCCCCCCCCCCCEECCHHCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCC
GGSIRQPAAFTGTVGIKPTYGRCSRWGTVAFASSLDQAGPIARDVRDAAILLKSMASVDA
CCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
KDTTSVDLPVPDYEAALGQSLKGMKIGIPSEYRVDGMPEEIETLWRQGIAWLKDAGAEIV
CCCCEEECCCCCHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEE
DISLPHTKYALPAYYIVAPAEASSNLARYDGVRYGLRVDGKDIVDMYEKTRAAGFGKEVK
EEECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
RRIMIGTYVLSAGYYDAYYIRAQKVRTLIKRDFELAFNAGVDAILTPATPSSAFGVADEN
HHHHHHHHEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCC
LASDPVKMYLNDIFTVTVNMAGLPGIAVPAGLDQKGLPLGLQLIGKPFDEETLFKTAHVI
CCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHH
EQAAGRFTPARWW
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA