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The map label for this gene is minD

Identifier: 209400798

GI number: 209400798

Start: 1602707

End: 1603519

Strand: Reverse

Name: minD

Synonym: ECH74115_1660

Alternate gene names: 209400798

Gene position: 1603519-1602707 (Counterclockwise)

Preceding gene: 209395883

Following gene: 209399824

Centisome position: 28.78

GC content: 49.69

Gene sequence:

>813_bases
ATGGCACGCATTATTGTTGTTACTTCGGGCAAAGGGGGTGTTGGTAAGACAACCTCCAGCGCGGCCATCGCCACTGGTTT
GGCCCAGAAGGGAAAGAAAACTGTCGTGATAGATTTTGATATCGGCCTGCGTAATCTCGACCTGATTATGGGTTGTGAAC
GCCGGGTCGTTTACGATTTCGTCAACGTCATTCAGGGCGATGCAACGCTAAATCAGGCGTTAATTAAAGATAAGCGTACT
GAAAATCTCTATATTCTGCCGGCATCGCAAACACGCGATAAAGATGCCCTCACCCGTGAAGGGGTCGCTAAAGTTCTTGA
TGATCTGAAAGCGATGGATTTTGAATTTATCGTTTGTGACTCCCCGGCAGGGATTGAAACCGGTGCGTTAATGGCACTCT
ATTTTGCAGACGAAGCCATTATTACCACCAACCCGGAAGTCTCCTCAGTACGCGACTCTGACCGTATTTTAGGCATTCTG
GCGTCAAAATCACGCCGCGCAGAAAATGGCGAAGAGCCTATTAAAGAGCACCTGCTGTTAACGCGCTATAACCCAGGCCG
CGTAAGCAGAGGTGACATGCTGAGCATGGAAGATGTGCTGGAGATCCTGCGCATCAAACTCGTCGGCGTGATCCCAGAGG
ATCAATCAGTATTGCGCGCCTCTAACCAGGGTGAACCGGTCATTCTCGACATTAACGCCGATGCGGGTAAAGCCTACGCA
GATACCGTAGAACGTCTGTTGGGAGAAGAACGTCCTTTCCGCTTCATTGAAGAAGAGAAGAAAGGCTTCCTCAAACGCTT
GTTCGGAGGATAA

Upstream 100 bases:

>100_bases
TCCCAGCAGAATTTTATGGCAAAGCGGCGCGACTGCAGTTAGTCGAAAACGCTTTGACCGTTCAACCGTTAAATTGATCC
CTTTTTAACAAGGAATTTCT

Downstream 100 bases:

>100_bases
GTTATGGCATTACTCGATTTCTTTCTCTCGCGGAAGAAAAATACAGCCAACATTGCAAAAGAACGGCTGCAGATTATTGT
TGCTGAACGCCGTCGCAGCG

Product: cell division inhibitor MinD

Products: NA

Alternate protein names: Cell division inhibitor minD

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRT
ENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYA
DTVERLLGEERPFRFIEEEKKGFLKRLFGG

Sequences:

>Translated_270_residues
MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRT
ENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYA
DTVERLLGEERPFRFIEEEKKGFLKRLFGG
>Mature_269_residues
ARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTE
NLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILA
SKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYAD
TVERLLGEERPFRFIEEEKKGFLKRLFGG

Specific function: ATPase required for the correct placement of the division site. Cell division inhibitors minC and minD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to desta

COG id: COG2894

COG function: function code D; Septum formation inhibitor-activating ATPase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the parA family. MinD subfamily

Homologues:

Organism=Escherichia coli, GI1787423, Length=270, Percent_Identity=100, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): MIND_ECO57 (P0AEZ5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E90837
- PIR:   F85695
- RefSeq:   NP_287414.1
- RefSeq:   NP_309696.1
- ProteinModelPortal:   P0AEZ5
- SMR:   P0AEZ5
- MINT:   MINT-137071
- EnsemblBacteria:   EBESCT00000025876
- EnsemblBacteria:   EBESCT00000056922
- GeneID:   913204
- GeneID:   959888
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1937
- KEGG:   ecs:ECs1669
- GeneTree:   EBGT00050000010759
- HOGENOM:   HBG691818
- OMA:   RITRRIL
- ProtClustDB:   PRK10818
- BioCyc:   ECOL83334:ECS1669-MONOMER
- InterPro:   IPR002586
- InterPro:   IPR010223
- TIGRFAMs:   TIGR01968

Pfam domain/function: PF01656 CbiA

EC number: NA

Molecular weight: Translated: 29614; Mature: 29483

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDF
CEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCHHHHHHH
VNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCD
HHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEC
SPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLL
CCCCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCEEE
TRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYA
EECCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEEECCCCCCHHH
DTVERLLGEERPFRFIEEEKKGFLKRLFGG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLRNLDLIMGCERRVVYDF
EEEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCHHHHHHH
VNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGVAKVLDDLKAMDFEFIVCD
HHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEC
SPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGILASKSRRAENGEEPIKEHLLL
CCCCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCEEEEECCCCCCCCCCHHHHHHCEEE
TRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVLRASNQGEPVILDINADAGKAYA
EECCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCHHHHHHCCCCCEEEEEECCCCCCHHH
DTVERLLGEERPFRFIEEEKKGFLKRLFGG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796