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The map label for this gene is mazG

Identifier: 209400744

GI number: 209400744

Start: 3736690

End: 3737481

Strand: Reverse

Name: mazG

Synonym: ECH74115_4041

Alternate gene names: 209400744

Gene position: 3737481-3736690 (Counterclockwise)

Preceding gene: 209400651

Following gene: 209398762

Centisome position: 67.08

GC content: 51.39

Gene sequence:

>792_bases
ATGAATCAAATCGACCGTTTGCTCACTATTATGCAGCGCCTGCGCGACCCGGAAAACGGCTGCCCGTGGGATAAAGAGCA
GACATTTGCCACCATTGCGCCTTACACCCTTGAAGAAACCTACGAAGTGCTGGACGCCATCGCCCGTGAAGATTTTGACG
ATCTGCGCGGTGAACTCGGCGATCTGCTGTTCCAGGTGGTGTTTTACGCCCAAATGGCTCAGGAAGAAGGGCGCTTTGAC
TTTAATGATATTTGCGCTGCGATTAGCGATAAATTAGAGCGTCGCCATCCGCATGTTTTTGCTGACAGTTCTGCCGAAAA
CAGTAGTGAAGTGCTTGCCCGTTGGGAGCAAATCAAAACCGAAGAGCGCGCGCAGAAAGCGCAGCATTCGGCGCTGGACG
ATATTCCTCGTAGTTTACCGGCTTTAATGCGTGCGCAAAAAATCCAGAAACGTTGCGCCAACGTTGGCTTCGATTGGACG
ACGCTTGGTCCGGTAGTCGATAAAGTCTACGAAGAGATCGACGAGGTGATGTACGAAGCGCGGCAGGCTGTTGTCGACCA
GGCTAAACTGGAGGAGGAAATGGGGGACCTGCTGTTTGCCACGGTCAATCTGGCTCGTCATTTAGGGACAAAAGCGGAAA
TCGCATTGCAAAAAGCGAACGAAAAATTCGAGCGTCGTTTTCGCGAAGTGGAGCGTATTGTTGCCGCGCGTGGACTGGAA
ATGACAGGTGTTGACCTCGAAACAATGGAAGAAGTCTGGCAACAGGTAAAACGGCAGGAAATTGATCTCTAA

Upstream 100 bases:

>100_bases
AAAGCCAAAATTAACGTACTGATTGGGTAGTGTTACTAACTCACTTCTATTCTGGTCACAGGTTTCCACCTGACGGACCC
GCAATTTTCAGGACATTCTA

Downstream 100 bases:

>100_bases
GGGAATTACGCGGTCAAGCGCCATTTGTGTCATTTTTTAAATGACAAGCGCTTGATTTGCGTCAAAAACATTTACCCCAA
AGGGGCTATTTTCTCACTCC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFD
FNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLE
MTGVDLETMEEVWQQVKRQEIDL

Sequences:

>Translated_263_residues
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFD
FNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLE
MTGVDLETMEEVWQQVKRQEIDL
>Mature_263_residues
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFD
FNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLE
MTGVDLETMEEVWQQVKRQEIDL

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family

Homologues:

Organism=Escherichia coli, GI1789144, Length=263, Percent_Identity=100, Blast_Score=539, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MAZG_ECO57 (P0AEY4)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98084
- PIR:   B85929
- RefSeq:   NP_289335.1
- RefSeq:   NP_311668.1
- ProteinModelPortal:   P0AEY4
- SMR:   P0AEY4
- MINT:   MINT-1311198
- EnsemblBacteria:   EBESCT00000025295
- EnsemblBacteria:   EBESCT00000058517
- GeneID:   916566
- GeneID:   958247
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4096
- KEGG:   ecs:ECs3641
- GeneTree:   EBGT00050000011615
- HOGENOM:   HBG616899
- OMA:   GFDWPDA
- ProtClustDB:   PRK09562
- BioCyc:   ECOL83334:ECS3641-MONOMER
- InterPro:   IPR004518
- InterPro:   IPR011551
- TIGRFAMs:   TIGR00444

Pfam domain/function: PF03819 MazG

EC number: =3.6.1.8

Molecular weight: Translated: 30413; Mature: 30413

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: BINDING 175-175 BINDING 196-196 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELG
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
DLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHH
EERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWTTLGPVVDKVYEEIDEVMYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTGVDLETMEEVWQQVKRQEIDL
CCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVLDAIAREDFDDLRGELG
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHH
DLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFADSSAENSSEVLARWEQIKT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHH
EERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWTTLGPVVDKVYEEIDEVMYEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQKANEKFERRFREVERIVAARGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MTGVDLETMEEVWQQVKRQEIDL
CCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796