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The map label for this gene is fruB

Identifier: 209400685

GI number: 209400685

Start: 3038656

End: 3039786

Strand: Reverse

Name: fruB

Synonym: ECH74115_3305

Alternate gene names: 209400685

Gene position: 3039786-3038656 (Counterclockwise)

Preceding gene: 209396849

Following gene: 209396003

Centisome position: 54.55

GC content: 55.97

Gene sequence:

>1131_bases
ATGTTCCAGTTATCCGTACAGGACATCCATCCGGGCGAAAAGGCCGGAGACAAAGAAGAGGCGATTCGCCAGGTCGCTGC
GGCGCTGGTGCAGGCCGGTAATGTAGCAGAAGGCTACGTCAATGGCATGCTGGCGCGCGAACAGCAAACCTCAACGTTCC
TCGGCAATGGTATTGCTATTCCACACGGCACTACCGACACCCGCGATCAGGTGCTGAAAACCGGCGTTCAGGTATTTCAG
TTCCCGGAAGGCGTCACCTGGGGTGACGGTCAGGTAGCGTACGTAGCGATCGGTATTGCTGCCAGCTCGGATGAGCATCT
GGGCCTGCTACGCCAGCTGACCCACGTACTGAGCGATGATTCCGTTGCTGAACAACTGAAGTCAGCAACAACAGCAGAAG
AACTTCGCGCATTGCTGATGGGCGAAAAGCAGAGTGAGCAGCTGAAGCTCGACAACGAAATGCTGACGCTGGATATCGTC
GCCAGCGATCTGCTGACTCTTCAGGCGCTGAACGCTGCGCGTCTGAAAGAGGCGGGGGCAGTTGACGCCACTTTCGTCAC
CAAAGCCATCAATGAACAACCGCTGAACCTCGGACAGGGTATCTGGCTGAGCGATAGCGCCGAAGGCAATCTGCGTAGCG
CAATTGCGGTAAGCCGTGCGGCAAATGCTTTTGATGTGGACGGCGAAACGGCAGCCATGCTGGTGAGTGTGGCGATGAAT
GACGATCAGCCCATTGCGGTTCTTAAGCGTCTCGCTGATTTGTTGCTCGACAATAAAGCTGACCGCTTGCTGAAAGCGGA
TGCGGCAACGTTGCTGGCGCTGCTGACCAGCGATGATGCGCCGACCGACGACGTGTTAAGCGCGGAGTTTGTGGTGCGCA
ATGAACACGGCCTGCATGCTCGTCCAGGTACCATGCTGGTCAACACCATTAAACAATTTAACAGTGATATTACCGTGACA
AACCTTGATGGCACCGGCAAACCGGCAAACGGACGTAGTCTGATGAAAGTTGTGGCACTTGGCGTTAAGAAAGGTCATCG
CCTACGCTTTACCGCCCAGGGTGCAGATGCTGAACAGGCGCTGAAAGCAATCGGCGACGCTATCGCTGCTGGTCTTGGGG
AGGGCGCATAA

Upstream 100 bases:

>100_bases
TGAAACGTTTCAGCGCGATCTTGTCTTTAACCCTAAGCCAGGTTGGCGCTTTTTTTCTCATAGAAGCTGAATCGTTTCAA
TTCAGCAAGAGAGGAGAACT

Downstream 100 bases:

>100_bases
TGAGCAGACGTGTTGCTACTATCACCCTTAATCCGGCTTATGACCTTGTTGGTTTCTGCCCGGAAATTGAACGCGGCGAA
GTGAACCTGGTGAAAACCAC

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA

Sequences:

>Translated_376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA
>Mature_376_residues
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQ
FPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVT
NLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain

Homologues:

Organism=Escherichia coli, GI1788494, Length=376, Percent_Identity=100, Blast_Score=750, Evalue=0.0,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=46.2068965517241, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTFAH_ECO57 (P69812)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91011
- RefSeq:   NP_288752.1
- RefSeq:   NP_311088.1
- ProteinModelPortal:   P69812
- EnsemblBacteria:   EBESCT00000023896
- EnsemblBacteria:   EBESCT00000059102
- GeneID:   916765
- GeneID:   958260
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3427
- KEGG:   ecs:ECs3061
- GeneTree:   EBGT00050000009675
- HOGENOM:   HBG511960
- OMA:   AIPHGTL
- ProtClustDB:   PRK11109
- BioCyc:   ECOL83334:ECS3061-MONOMER
- GO:   GO:0005737
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- Gene3D:   G3DSA:3.40.930.10
- Gene3D:   G3DSA:3.30.1340.10
- PRINTS:   PR00107
- TIGRFAMs:   TIGR01003

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2; SSF55594 HPr_protein; SSF55804 PTrfase/Anion_transptr

EC number: 2.7.1.69

Molecular weight: Translated: 39648; Mature: 39648

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS51094 PTS_EIIA_TYPE_2; PS00372 PTS_EIIA_TYPE_2_HIS; PS51350 PTS_HPR_DOM; PS00369 PTS_HPR_HIS; PS00589 PTS_HPR_SER

Important sites: ACT_SITE 62-62 ACT_SITE 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIVASDLLTLQALNAARLKEAGA
HHHHHHHHCCCHHHHHHHHHCCCCCHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
VDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
CHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEECC
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQA
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGDAIAAGLGEGA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGMLAREQQTSTFLGNGIAI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
PHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAASSDEHLGLLRQLTHVLSDD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCC
SVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIVASDLLTLQALNAARLKEAGA
HHHHHHHHCCCHHHHHHHHHCCCCCHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCC
VDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVSRAANAFDVDGETAAMLVSVAMN
CHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEECC
DDQPIAVLKRLADLLLDNKADRLLKADAATLLALLTSDDAPTDDVLSAEFVVRNEHGLHA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCC
RPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSLMKVVALGVKKGHRLRFTAQGADAEQA
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
LKAIGDAIAAGLGEGA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796