| Definition | Escherichia coli O157:H7 str. EC4115, complete genome. |
|---|---|
| Accession | NC_011353 |
| Length | 5,572,075 |
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The map label for this gene is menF [H]
Identifier: 209400654
GI number: 209400654
Start: 3151223
End: 3152518
Strand: Reverse
Name: menF [H]
Synonym: ECH74115_3407
Alternate gene names: 209400654
Gene position: 3152518-3151223 (Counterclockwise)
Preceding gene: 209398447
Following gene: 209399910
Centisome position: 56.58
GC content: 53.63
Gene sequence:
>1296_bases GTGCAATCACTTACTACGGCGCTGGAAAATCTACTGCGCCATTTGTCGCAAGAGATTCCGGCGACACCCGGCATTCGGGT TATCGATATTCCTTTCCCTCTCAAAGACGCTTTTGATGCCTTGAGCTGGCTGGCCAGTCAGCAAACGTACCCGCAATTCT ACTGGCAACAACGTAATGGTGATGAAGAAGCTGCCGTCCTGGGCGCGATTACCCGTTTTACGTCGTTGGACCAGGCACAA CGTTTTCTTCGCCAGCACCCGGAACACGCCGACTTACGCATTTGGGGGCTGAATGCGTTTGACCCGTCGCAGGGCAATTT ACTTTTACCACGCCTGGAATGGCGACGCTGTGGCGGTAAAGCCACGCTGCGGCTGACGCTATTCAGCGAAAGCTCCCTTC AGCACGATGCGATTAAGGCAAGAGAATTTATCGCCACACTGGTGAGTATCAAGCCCTTGCCTGGGTTACATTTAACCACT ACGCGAGAACAACACTGGCCGGACAAAACGGGCTGGACGCAATTAATCGAACTGGCAACGAAAACCATCGCCGAAGGTGA GCTCGACAAAGTGGTGCTTGCTCGGGCAACTGACCTGCATTTCGCAAGTCCGGTCAATGCGGCGGCGATGATGGCTGCCA GTCGTCGACTGAATCTCAATTGCTACCATTTTTACATGGCCTTTGATGGCGAAAATGCTTTTCTTGGCTCTTCACCGGAA CGGTTATGGCGGCGGCGTGACAAAGCGCTGCGTACTGAAGCGCTGGCGGGAACAGTAGCAAATCATCCTGATGATAAGCA GGCGCAGCAGTTAGGAGAGTGGCTGATGGCGGATGATAAAAACCAGCGCGAGAACATGCTGGTGGTGGAAGATATCTGCC AACGATTACAGGCCGATACCCAGACGCTGGATGTTTTACCGCCGCAGGTACTGCGTCTGCGTAAAGTGCAGCATCTTCGC CGCTGTATCTGGACTTCACTCAACAAAGCGGATGATGTGATCTGTTTACATCAGTTGCAGCCAACGGCAGCAGTTGCTGG CTTACCGCGCGATCTGGCGCGACAGTTTATCGCCCATCACGAACCGTTCACCCGAGAATGGTACGCCGGTTCTGCGGGCT ATCTCTCATTACAACAAAGCGAATTCTGCGTTTCCCTGCGCTCAGCAAAAATCAGCGGCAATGTCGTGCGGCTATATGCT GGCGCGGGCATTGTCCGTGGTTCCGACCCCGAGCAAGAGTGGCAGGAAATCGACAACAAAGCGGCAGGGCTGCGTACTTT ATTACAAATGGAATAG
Upstream 100 bases:
>100_bases ACTGTTGCTGGCACGCCGTTAAAACCTCCCTACACCGGGGTACTGCATTTTTTTTGTAGTACCCCGTATAATGTGAGGCT TTTAACAGGGAGAGGTCCGC
Downstream 100 bases:
>100_bases TAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAATATTGATACCGG ACAAACTCATGTCAGTAAGC
Product: isochorismate synthase, menaquinone-specific
Products: NA
Alternate protein names: Isochorismate mutase [H]
Number of amino acids: Translated: 431; Mature: 431
Protein sequence:
>431_residues MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE RLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME
Sequences:
>Translated_431_residues MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE RLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME >Mature_431_residues MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE RLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME
Specific function: Menaquinone biosynthesis. [C]
COG id: COG1169
COG function: function code HQ; Isochorismate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the isochorismate synthase family [H]
Homologues:
Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=98.8399071925754, Blast_Score=879, Evalue=0.0, Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=31.5217391304348, Blast_Score=131, Evalue=9e-32, Organism=Escherichia coli, GI1787518, Length=299, Percent_Identity=27.7591973244147, Blast_Score=71, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6320935, Length=327, Percent_Identity=27.5229357798165, Blast_Score=76, Evalue=1e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005801 - InterPro: IPR015890 - InterPro: IPR004561 [H]
Pfam domain/function: PF00425 Chorismate_bind [H]
EC number: =5.4.4.2 [H]
Molecular weight: Translated: 48769; Mature: 48769
Theoretical pI: Translated: 7.17; Mature: 7.17
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC ATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE HHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH RLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADT HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIAHH HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK CCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCHHHHHHHHHH AAGLRTLLQME HHHHHHHHHCC >Mature Secondary Structure MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC ATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE HHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH RLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADT HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIAHH HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK CCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCHHHHHHHHHH AAGLRTLLQME HHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]