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The map label for this gene is ureF1

Identifier: 209400407

GI number: 209400407

Start: 1337665

End: 1338339

Strand: Direct

Name: ureF1

Synonym: ECH74115_1325

Alternate gene names: 209400407

Gene position: 1337665-1338339 (Clockwise)

Preceding gene: 209399946

Following gene: 209398478

Centisome position: 24.01

GC content: 59.11

Gene sequence:

>675_bases
ATGCCGACTCCGGAAAAACGTCTGCGGCTGATGCAGCTTGCCAGTAACAGCCTCCCTGTTGGAGGCTACAGCTGGTCCCA
GGGACTGGAGTGGGCAGTGGAAGCAGGCTGGGTAGAAGATAGCGCCGCCTTTGAACACTGGCAGCAGTTGCAGATGGAAC
AGAGTTTTTTTGCCGTCGACCTGCCACTGCTTGCCCGGCTATACCGGGCCTGTGAAGCGGGCGATCCTGACAGTGCCGGG
CGCTGGACCGCTTATCTGCTGGCCTGCAGGGAAACCCGTGAGCTACGCGATGAAGAACGTAACCGCGGTGCAGCCTTCAC
CAGACTTCTGGTCGACTGGCAGCCCGACTGCCCGGCCGAATGGCGCAAACTATGCCAGCAAAGCCAGTTAACCGGTATGG
CATGGCTCGGCGTACGCTGGCAAATATCCGTTTCAGACCTGGCCCTGAGCCTTGGCTACAGCTGGATTGAAAGCGCAGTG
ATGGCGGGCGTCAGGCTGGTACCCTACGGCCAGCTGGCTGCCCAGCAGTTAATTATGCGCCTGTGTGCCCGCTATGCGGC
GAACATGGACAGTGCCCTTGCAACCCCGGATCACGCAATTGGTTCAGCAACGCCACTTGCATCTATCGCTTCCGCGCGAC
ATGAAACCCAGTATTCCCGATTATTCCGTTCTTAG

Upstream 100 bases:

>100_bases
GCTGGATGTTGATTTTGCACATCTTCCGTTTGAGCCGGAAGCCGGCGCTTACGCCAGCAAGTCGCACGCCCATAATCACG
ATCAGGAGCACAGCCACTGA

Downstream 100 bases:

>100_bases
GAGAAAATAAAATGAACATAATCAAGCAACCGCTGCGTGTCGGCGTCGGCGGTCCGGTCGGCTCAGGTAAAACCGCGCTT
CTGGAAGCCCTCTGCAAATC

Product: UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAFEHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAG
RWTAYLLACRETRELRDEERNRGAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQYSRLFRS

Sequences:

>Translated_224_residues
MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAFEHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAG
RWTAYLLACRETRELRDEERNRGAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQYSRLFRS
>Mature_223_residues
PTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAFEHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGR
WTAYLLACRETRELRDEERNRGAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAVM
AGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_ECO57 (Q8X9U5)

Other databases:

- EMBL:   AE005174
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85655
- PIR:   F90794
- RefSeq:   NP_286682.1
- RefSeq:   NP_287090.1
- RefSeq:   NP_309353.1
- ProteinModelPortal:   Q8X9U5
- EnsemblBacteria:   EBESCT00000027888
- EnsemblBacteria:   EBESCT00000028960
- EnsemblBacteria:   EBESCT00000060470
- GeneID:   912425
- GeneID:   958588
- GeneID:   959233
- GenomeReviews:   AE005174_GR
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1147
- KEGG:   ece:Z1586
- KEGG:   ecs:ECs1326
- GeneTree:   EBGT00050000012668
- HOGENOM:   HBG709295
- OMA:   GFAWLEG
- ProtClustDB:   CLSK879808
- BioCyc:   ECOL83334:ECS1326-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 25156; Mature: 25025

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAFEHWQQLQMEQSFFAVD
CCCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNRGAAFTRLLVDWQPDCPAE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHH
WRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAVMAGVRLVPYGQLAAQQLIMR
HHHHHHHHHCCCEEEECEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LCARYAANMDSALATPDHAIGSATPLASIASARHETQYSRLFRS
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAFEHWQQLQMEQSFFAVD
CCHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNRGAAFTRLLVDWQPDCPAE
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHH
WRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAVMAGVRLVPYGQLAAQQLIMR
HHHHHHHHHCCCEEEECEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LCARYAANMDSALATPDHAIGSATPLASIASARHETQYSRLFRS
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796